Mitosis and Meiosis

Other | December 1, 1998

byWilson, Leslie, Leslie Wilson

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Mitosis and Meiosisdetails the wide variety of methods currently used to study how cells divide as yeast and insect spermatocytes, higher plants, and sea urchin zygotes. With chapters covering micromanipulation of chromosomes and making, expressing, and imaging GFP-fusion proteins, this volume contains state-of-the-art "how to" secrets that allow researchers to obtain novel information on the biology of centrosomes and kinetochores and how these organelles interact to form the spindle.

Chapters Contain Information On:
* How to generate, screen, and study mutants of mitosis in yeast, fungi, and flies
* Techniques to best image fluorescent and nonfluorescent tagged dividing cells
* The use and action of mitoclastic drugs
* How to generate antibodies to mitotic components and inject them into cells
* Methods that can also be used to obtain information on cellular processes in nondividing cells

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From the Publisher

Mitosis and Meiosisdetails the wide variety of methods currently used to study how cells divide as yeast and insect spermatocytes, higher plants, and sea urchin zygotes. With chapters covering micromanipulation of chromosomes and making, expressing, and imaging GFP-fusion proteins, this volume contains state-of-the-art "how to" secrets...

Format:OtherDimensions:489 pages, 1 × 1 × 1 inPublished:December 1, 1998Publisher:Academic PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:0080859593

ISBN - 13:9780080859590

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Table of Contents

M. Moritz and B.M. Alberts, Isolation of Centrosomes fromDrosophilaEmbryos.
M. Bornens and M. Moudjou, Studying the Composition and Function of Centrosomes in Vertebrates.
R.E. Palazzo and J.M. Vogel, Isolation of Centrosomes fromSpisula solidissimaOocytes.
A. Van Hooser and B.R. Brinkley, Methods for thein SituLocalization of Proteins and DNA in the Centromere-Kinetochore Complex.
B.F. McEwen and M. Marko, Three-Dimensional Transmission Electron Micorscopy and Its Application to Mitosis Research.
K.F. Sullivan, Enlightening Mitosis: Construction and Expression of GFP Fusion Proteins.
T. Wittmann and T. Hyman, Recombinant p50/Dynamitin as a Tool to Examine the Role of Dynactin in Intracellular Processes.
F. Severin, K. Kaplan, P. Sorger, and T. Hyman, In VitroAssays for StudyingS. cerevisiaeKinetochore Activity.
C. Waterman-Storer, A. Desai, and E.D. Salmon, Fluorescent Speckle Microscopy of Spindle Microtubule Assembly and Motility in Living Cells.
R. Oldenbourg, Polarized Microscopy of Spindles.
D. Zhang and R.B. Nicklas, Micromanipulation of Chromosomes and Spindles in Insect Spermatocytes.
P. Wadsworth, Microinjection of Mitotic Cells.
R. Kuriyama and K. Ensrud, Obtaining Antibodies to Spindle Components.
L. Wordeman and M. Wagenbach, Using Antisense Technology to Study Mitosis.
M.A. Jordan and L. Wilson, The Use and Action of Drugs in Analyzing Mitosis.
C.L. Rieder and G. Cassels, Correlative Light and Electron Microscopy of Mitotic Cells in Monolayer Cultures.
W.E. Theurkauf and M.M.S. Heck, Identification and Characterization of Mitotic Mutations inDrosophila.
B.R. Oakley, Methods of Isolating and Analyzing Mitotic Mutants inAspergillus nidulans.
K. Bloom, D.L. Beach, P. Maddox, S.L. Shaw, E. Yeh, and E.D. SalmonUsing GFP Fusion Proteins to Quantitate Microtubule and Spindle Dynamics in Budding Yeast.
A. Desai, A. Murray, T. Mitchison, and C.E. Walczak, The Use ofXenopusEgg Extracts to Study Mitotic Spindle Assembly and Functionin Vitro.
J.W. Voss, A.H. Valster, and P.K. Hepler, Methods for Studying Cell Division in Higher Plants.
G. Sluder, F.J. Miller, and E. Hinchcliffe, Using Sea Urchin Gametes for the Study of Mitosis.
Chapter References.
Index.