Mobile Genetic Elements by Wolfgang J. MillerMobile Genetic Elements by Wolfgang J. Miller

Mobile Genetic Elements

EditorWolfgang J. Miller, Pierre Capy

Paperback | November 10, 2010

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Leading experts describe in step-by-step detail their most productive transposon-based methods and strategies for studying genome structure, function, and evolution. These readily reproducible techniques cover a wide range, including mutagenesis, transgenesis, gene silencing, and molecular systematics. Among the highlights are a series of DNA hybridization methods for analyzing the distribution and dynamics of mobile DNA at the hosts' genomic level, techniques for studying LTR retrotransposons in heterologous host systems, and mutagenesis protocols for investigating gene functions in a broad range of organisms. These cutting-edge methods offer investigators powerful genetic tools for dissecting the function of a specific gene, elaborating on the mechanisms leading to genetic change and diversity, and studying the evolutionary impact of mobile DNA on the biology and evolution of organisms.
Title:Mobile Genetic ElementsFormat:PaperbackDimensions:302 pages, 9.02 × 5.98 × 0 inPublished:November 10, 2010Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:161737301X

ISBN - 13:9781617373015

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Table of Contents

Mobile Genetic Elements as Natural Tools for Genome EvolutionWolfgang J. Miller and Pierre CapyDetection of Transposable Elements in Drosophila Salivary Gland Polytene Chromosomes by In Situ HybridizationChristian Biémont, Laurence Monti-Dedieu, and Françoise LemeunierFluorescent In Situ Hybridization With Transposable Element Probes to Mitotic Chromosomal Heterochromatin of DrosophilaPatrizio DimitriSouthern Blot Analysis of Individual Drosophila FliesNikolaj JunakovicComputational Analysis of Transposable Element SequencesI. King Jordan and Nathan J. BowenBiochemical Analysis of Long Terminal Repeat RetrotransposonsAndreas Bachmair, Karin Garber, Shin Takeda, Kazuhiko Sugimoto, Tetsuji Kakutani, and Hirohiko HirochikaTn5 as a Molecular Genetics Tool: In Vitro Transposition and the Coupling of In Vitro Technologies With In Vivo TranspositionWilliam S. Reznikoff, Igor Y. Goryshin, and Jerry J. JendrisakGene Targeting in DrosophilaGregory B. GloorTE-Based Mutagenesis Systems in Plants: A Gene Family ApproachMichiel Vandenbussche and Tom GeratsThe Use of Double-Stranded RNA to Knock Down Specific Gene ActivityMary K. MontgomeryThe Application of LTR Retrotransposons as Molecular Markers in PlantsAlan H. Schulman, Andrew J. Flavell, and T. H. Noel EllisMITE DisplayAlexandra M. Casa, Alexander Nagel, and Susan R. WesslerRetroposon Mapping in Molecular SystematicsNorihiro Okada, Andrew M. Shedlock, and Masato NikaidoTransformation Systems in InsectsDavid A. O´Brochta and Peter W. AtkinsonTransposable Elements for Transgenesis and Insertional Mutagenesis in Vertebrates: A Contemporary Review of Experimental StrategiesZoltán Ivics and Zsuzsanna IzsvákIndex