Mobile Genetic Elements by Wolfgang J. MillerMobile Genetic Elements by Wolfgang J. Miller

Mobile Genetic Elements

byWolfgang J. MillerEditorPierre Capy

Paperback | November 10, 2010

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Mobile Genetic Elements: Protocols and Genomic Applications brings together a wide array of transposon-based protocols and stategies for studying genome structure, function, and evolution into a highly practical, single-source volume. Such transposable element (TE)-derived techniques have been applied succe- fully for a variety of purposes ranging from mutagenesis, gene silencing, transgenesis, and their use as polymorphic marker systems. To our knowledge no such synthesis has been presented before. Chapters 2-4 provide a series of DNA hybridization techniques for anal- ing the distribution and dynamics of mobile DNAs at the hosts' genomic level. With the current revolution in genomics and the availability of complete genome sequences, computational analyses provide an extremely powerful tool for i- lating and investigating TEs at the in silico level (Chapter 5). For the analyses of transpositional mechanisms at the biochemical level Chapter 6 provides a detailed protocol for LTR retrotransposons in heterologous host systems. Ch- ters 7-10 are focused on TE-based mutagenesis protocols for studying gene functions in a broad range of organisms. Based on their ubiquitous nature and their activity in creating genomic diversity by integrating novel DNA segments into genomes, TEs provide highly informative sets of polymorphic markers (Chapters 11-13). Finally, the last two chapters are dedicated to their technical applications during transgenesis in arthropods and vertebrates.
Title:Mobile Genetic ElementsFormat:PaperbackDimensions:292 pagesPublished:November 10, 2010Publisher:Springer-Verlag/Sci-Tech/TradeLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:161737301X

ISBN - 13:9781617373015

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Table of Contents

Mobile Genetic Elements as Natural Tools for Genome Evolution Wolfgang J. Miller and Pierre Capy Detection of Transposable Elements in Drosophila Salivary Gland Polytene Chromosomes by In Situ Hybridization Christian Biémont, Laurence Monti-Dedieu, and Françoise Lemeunier Fluorescent In Situ Hybridization With Transposable Element Probes to Mitotic Chromosomal Heterochromatin of Drosophila Patrizio Dimitri Southern Blot Analysis of Individual Drosophila Flies Nikolaj Junakovic Computational Analysis of Transposable Element Sequences I. King Jordan and Nathan J. Bowen Biochemical Analysis of Long Terminal Repeat Retrotransposons Andreas Bachmair, Karin Garber, Shin Takeda, Kazuhiko Sugimoto, Tetsuji Kakutani, and Hirohiko Hirochika Tn5 as a Molecular Genetics Tool: In Vitro Transposition and the Coupling of In Vitro Technologies With In Vivo Transposition William S. Reznikoff, Igor Y. Goryshin, and Jerry J. Jendrisak Gene Targeting in Drosophila Gregory B. Gloor TE-Based Mutagenesis Systems in Plants: A Gene Family Approach Michiel Vandenbussche and Tom Gerats The Use of Double-Stranded RNA to Knock Down Specific Gene Activity Mary K. Montgomery The Application of LTR Retrotransposons as Molecular Markers in Plants Alan H. Schulman, Andrew J. Flavell, and T. H. Noel Ellis MITE Display Alexandra M. Casa, Alexander Nagel, and Susan R. Wessler Retroposon Mapping in Molecular Systematics Norihiro Okada, Andrew M. Shedlock, and Masato Nikaido Transformation Systems in Insects David A. O´Brochta and Peter W. Atkinson Transposable Elements for Transgenesis and Insertional Mutagenesis in Vertebrates: A Contemporary Review of Experimental Strategies Zoltán Ivics and Zsuzsanna Izsvák Index