Molecular Analysis of Cancer by Jacqueline BoultwoodMolecular Analysis of Cancer by Jacqueline Boultwood

Molecular Analysis of Cancer

EditorJacqueline Boultwood, Carrie Fidler

Paperback | December 12, 2011

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Leading researchers describe in step-by-step detail their best state-of-the art molecular techniques for elucidating the biochemical processes associated with human cancer. Among the techniques presented for identifying chromosomal rearrangements are fluorescent in situ hybridization (FISH), spectral karyotyping (SKY), comparative genomic hybridization (CGH), microsatellite analysis, PCR and RT-PCR, and DNA fingerprinting. Additional methods include SSCP, DGGE, the nonisotopic RNase cleavage assay, and the protein truncation assay and DNA sequencing for identifying mutations.
Title:Molecular Analysis of CancerFormat:PaperbackDimensions:328 pages, 9.02 × 5.98 × 0 inPublished:December 12, 2011Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617371025

ISBN - 13:9781617371028

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Table of Contents

Molecular Analysis of Cancer: An OverviewKen MillsDetection of Chromosome Abnormalities in Leukemia Using Fluorescence In Situ HybridizationLyndal Kearney, Sabrina Tosi, and Rina J. JajuSpectral Karyotyping in Cancer CytogeneticsEva Hilgenfeld, Cristina Montagna, Hesed Padilla-Nash, Linda Stapleton, Kerstin Heselmeyer-Haddad, and Thomas RiedComparative Genomic Hybridization AnalysisBinaifer R. Balsara, Jianming Pei, and Joseph R. TestaDetection of Chromosomal Deletions by Microsatellite AnalysisRachel E. Ibbotson and Martin M. CorcoranDetection and Quantification of Leukemia-Specific RearrangementsAndreas HochhausDetection of t(2;5)(p23;q35) Translocation by Long-Range PCR of Genomic DNAYunfang Jiang, L. Jeffrey Medeiros, and Andreas H. SarrisUse of DNA Fingerprinting to Detect Genetic Rearrangements in Human CancerVorapan Sirivatanauksorn, Yongyut Sirivatanauksorn, Arthur B. McKie, and Nicholas R. LemoineMutation Analysis of Large Genomic Regions in Tumor DNA Using Single-Strand Conformation Polymorphism: Lessons from the ATM GeneIgor VorechovskyMutational Analysis of Oncogenes and Tumor Suppressor Genes in Human Cancer Using Denaturing Gradient Gel ElectrophoresisPer Guldberg, Kirsten Grønbæk, Jesper Worm, Per thor Straten, and Jesper ZeuthenDetection of Mutations in Human Cancer Using Nonisotopic RNase Cleavage AssayMarianna Goldrick and James PrescottMutational Analysis of the Neurofibromatosis Type 1 Gene in Childhood Myelodysplastic Syndromes Using a Protein Truncation AssayLucy SideMutation Analysis of Cancer Using Automated SequencingAmanda Strickson and Carrie FidlerDetection of Differentially Expressed Genes in Cancer Using Differential DisplayYineng FuGenomewide Gene Expression Analysis Using cDNA MicroarraysChuang Fong Kong and David BowtellGene Expression Profiling in Cancer Using cDNA MicroarraysJaved Khan, Lao H. Saal, Michael L. Bittner, Yuan Jiang, Gerald C. Gooden, Arthur A. Glatfelter, and Paul S. MeltzerWilms Tumor Gene WT1 as a Tumor Marker for Leukemic Blast Cells and Its Role in LeukemogenesisHaruo SugiyamaDetection of Aberrant Methylation of the p15INK4B Gene PromoterToshiki UchidaClonality Studies in Cancer Based on X Chromosome Inactivation PhenomenonJohn T. Phelan II and Josef T. PrchalTelomere Length Changes in Human CancerDominique Broccoli and Andrew K. GodwinMeasurement of Telomerase Activity in Human Hematopoietic Cells and Neoplastic DisordersKazuma Ohyashiki and Junko H. Ohyashiki

Editorial Reviews

"The book covers a broad range of topics and provides many useful suggestions as well as practical guidance for the lab researcher." -Doody's Health Sciences Book Review Journal"This book gives a good overview of currently available methods for investigating molecular changes in cancers,...it illustrates how you, the cancer researcher, can apply the methods to your oncology problem...The most enjoyable chapters also illustrate the methods with real results, so that you can see what to expect, e.g. chapter 3 on spectral karyotyping...a useful addition in any oncology research laboratory, because it presents this large variety of methods in a compact format, so that someone waiting to try a new approach could easily dip into the book to see what is available." - Molecular Biotechnology"Comprehensive and state-of-the-art, Molecular Analysis of Cancer not only offers today's researcher a deeper understanding of cancer's molecular pathogenesis, but also provides a detailed examination of each of the major methodologies needed to detect and analyze its associated genetic abnormalities." - Clinical Laboratory"...good integration between preliminary explanations of techniques and practical instructions with easily available tables or figures." - Proteomics