Molecular Cardiology: Methods and Protocols by Zhongjie SunMolecular Cardiology: Methods and Protocols by Zhongjie Sun

Molecular Cardiology: Methods and Protocols

EditorZhongjie Sun

Paperback | November 10, 2010

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The first major collection of techniques for gene therapy and stem cell therapy for heart diseases. The methods include readily reproducible techniques for genetic dissection, signal transduction, and microarray analysis. The protocols include techniques for gene transfer to heart tissue using viral and nonviral vectors and for producing transgenic models of heart diseases, the novel and powerful tools for the study of heart disease pathogenesis. Many of the protocols employ not only molecular and cell biology techniques, but also in vivo approaches to myocardial repair using different types of stem cells, and include methods for expansion, purification, and genetic modification of stem cells. The techniques are at the center of the most recent advances in the study and treatment of genetic and acquired heart diseases.
Title:Molecular Cardiology: Methods and ProtocolsFormat:PaperbackDimensions:436 pages, 9.02 × 5.98 × 0 inPublished:November 10, 2010Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617375217

ISBN - 13:9781617375217

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Table of Contents

Part I. IntroductionIntroduction to Methods in Molecular CardiologyZhongjie SunPart II. Gene Transfer and Gene Therapy for Cardiovascular DiseaseGene Therapy Approaches to Cardiovascular DiseaseJ. Mark Jones and Walter J. KochGene Therapy for Myocardial Ischemia Using the Hypoxia-Inducible Double Plasmid SystemYi TangCardiac Gene Delivery Using DNA-Adenoviral ConstructsJ. Mark Jones and Walter J. KochManipulation of Neuronal Nitric Oxide Synthase Within the Paraventricular Nucleus Using Adenovirus and Antisense TechnologyYi-Fan Li, Yu Wang, Keith M. Channon, Harold D. Schultz, Irving H. Zucker, and Kaushik P. PatelTAT-Mediated Protein Transduction: Delivering Biologically Active Proteins to the HeartÅsa B. Gustafsson, Roberta A. Gottlieb, and David J. GranvilleExpression of Human eNOS in Cardiac and Endothelial CellsXiuqing Wang, Robert Cade, and Zhongjie SunStrategies of Conditional Gene Expression in Myocardium: An OverviewHeather L. Heine, Hon Sing Leong, Fabio M. V. Rossi, Bruce M. McManus, and Thomas J. PodorGene Silencing Using Adenoviral RNAi Vector in Vascular Smooth Muscule Cells and CardiomyocytesHideko Kasahara and Hiroki AokiPart III. Stem Cell Therapy for Cardiovascular DiseaseCardiac Commitment of Embryonic Stem Cells for Myocardial RepairDana Zeineddine, Evangelia Papadimou, Annabelle Mery, Claudine Ménard, and Michel PucéatAutologous Mesenchymal Stem Cells for Post-Ischemic Myocardial RepairYao Liang TangCellular Therapy With Autologous Skeletal Myoblasts for Ischemic Heart Disease and Heart FailureYao Liang TangProtocols for Myocardial Infarction Repair Using Fetal Cardiac MyocytesSharon Etzion, Radka Holbova, Liron Miller, and Jonathan LeorMethods for Examining Stem Cells in Post-Ischemic and Transplanted HeartsNana Rezai, Hubert Walinski, Alexandra Kerjner, Lubos Bohunek, Fabio M. V. Rossi, Bruce M. McManus, and Thomas J. PodorEndothelial Progenitor Cell Culture and Gene TransferHideki Iwaguro and Takayuki AsaharaPart IV. Gene Analysis in the Injured and Hypertrophied HeartDelineation of Sequences Essential for Specific Promoter Activation During Pressure Overloaded Hypertrophy or Factor-Induced HypertrophyChellam Rajamanickam and Radhakrishnan Jeejabai.Evaluation of the Cardiac Isoform of a2-Macroglobulin as a Factor Inducing Cardiac HypertrophyChellam Rajamanickam and Radhakrishnan JeejabaiDetection of Apoptosis in Cardiovascular DiseasesArwen L. Hunter, Jonathan C. Choy, and David J. GranvilleDetection of Cardiac Signaling in the Injured and Hypertrophied HeartXiaoning Si, Maziar Rahmani, Ji Yuan, and Honglin LuoAffymetrix Oligonucleotide Analysis of Gene Expression in the Injured HeartBobby Yanagawa, Lydia Taylor, Theresa A. Deisher, Raymond Ng, George F. Schreiner, Timothy J. Triche, Decheng Yang, and Bruce M. McManusIdentification and Validation of Loci and Candidate Genes Linked to Cardiac HypertrophyBastien Llamas and Christian F. DeschepperInduction and Analysis of Cardiac Hypertrophy in Transgenic Animal ModelsMarcos E. Barbosa, Natalia Alenina, and Michael BaderMethods for the Detection of Altered b-Adrenergic Receptor Signaling Pathways in Hypertrophied HeartsMatthew J. Wolf, Hideo Tachibana, and Howard A. RockmanPart V. Transgenes in Cardiovascular ResearchTransgenic Mouse Models for Cardiac Dysfunction by a Specific Gene ManipulationGopal J. Babu and Muthu PeriasamyCardiomyocyte Defects in Diabetic Models and Protection With Cardiac-Targeted TransgenesXia Shen, Gang Ye, Naira S. Metreveli, and Paul N. EpsteinCardiac-Specific Overexpression of Angiotensin II Type 1 Receptor in Transgenic RatsSigrid HoffmannIndex