Molecular Diagnosis of Infectious Diseases by Jochen DeckerMolecular Diagnosis of Infectious Diseases by Jochen Decker

Molecular Diagnosis of Infectious Diseases

EditorJochen Decker

Paperback | November 10, 2010

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This second edition of a classic laboratory manual describes cutting-edge methods for the protein-based diagnosis of infectious diseases. Explaining the latest developments in genomics, proteomics, bioinformatics, biosensors, high-throughput devices, and recombinant technology, the authors apply these new methodologies successfully to the identification and characterization of valuable diagnostic markers, immunomodulatory components, epitope mapping, the production and purification of recombinant antigens, as well as to diagnostic reagents in immunological assays.
Title:Molecular Diagnosis of Infectious DiseasesFormat:PaperbackDimensions:480 pagesPublished:November 10, 2010Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:161737430X

ISBN - 13:9781617374302

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Table of Contents

Part I. Protein-Based Identification of AntigensProteomic Approaches to Antigen DiscoveryKaren M. Dobos, John S. Spencer, Ian M. Orme, and John T. BelisleImmunoproteomicsAlexander Krah and Peter R. JungblutImmunoprecipitation and Blotting: The Visualization of Small Amounts of Antigens Using Antibodies and LectinsStephen ThompsonPart II. DNA-Based Identification of AntigensRepresentational Difference Analysis of cDNALucas D. BowlerMicroarray Data Analysis and MiningSilvia Saviozzi, Giovanni Iazzetti, Enrico Caserta, Alessandro Guffanti, and Raffaele A. CalogeroExpression CloningMichael J. Lodes, Davin C. Dillon, Raymond L. Houghton, and Yasir A. W. SkeikyPart III. Identification of Epitopes and Immunomodulatory ComponentsDetermination of Epitopes by Mass SpectrometryChristine Hager-Braun and Kenneth B. TomerIdentification of T Cell Epitopes Using ELISpot and Peptide Pool ArraysTimothy W. Tobery and Michael J. CaulfieldVirus-like Particles: A Novel Tool for the Induction and Monitoring of Both T-Helper and Cytotoxic T-Lymphocyte ActivityLudwig Deml, Jens Wild, and Ralf WagnerApplication of Single-Cell Cultures of Mouse Splenocytes as an Assay System to Analyze the Immunomodulatory Properties of Bacterial ComponentsLudwig Deml, Michael Aigner, Alexander Eckhardt, Jochen Decker, Norbert Lehn, and Wulf Schneider-BrachertPart IV. Expression of Recombinant ProteinsHigh-Throughput Expression and Purification of 6xHis-Tagged Proteins in a 96-Well FormatJutta Drees, Jason Smith, Frank Schäfer, and Kerstin SteinertProduction of Antigens in Chlamydomonas reinhardtii: Green Microalgae as a Novel Source of Recombinant ProteinsMarkus FuhrmannCodon-Optimized Genes that Enable Increased Heterologous Expression in Mammalian Cells and Elicit Efficient Immune Responses in Mice after Vaccination of Naked DNAMarcus Graf, Ludwig Deml, and Ralf WagnerPart V. Purification, Modification, and Renaturation of Recombinant ProteinsPurification and Immunological Characterization of Recombinant Antigens Expressed in the Form of Insoluble Aggregates (Inclusion Bodies)Udo ReischlPurification of Recombinant Proteins with High Isoelectric PointsRaffaele A. Calogero and Anna AulicinoRefolding of Inclusion Body ProteinsMarcus Mayer and Johannes BuchnerSmall-Molecule-Protein Conjugation ProceduresStephen ThompsonPart VI. Characterization of Recombinant ProteinsStructural Characterization of Proteins and PeptidesRainer DeutzmannDetermination of Kinetic Data Using Surface Plasmon Resonance BiosensorsClaudia Hahnefeld, Stephan Drewianka, and Friedrich W. HerbergAffinity Measurements of Biological Molecules by a Quartz Crystal Microbalance (QCM) BiosensorUwe Schaible, Michael Liss, Elke Prohaska, Jochen Decker, Karin Stadtherr, and Hans WolfPart VII. Evaluation of Recombinant Proteins in Immunological Test SystemsSolid Supports in Enzyme-Linked Immunosorbent Assay and Other Solid-Phase ImmunoassaysJohn E. ButlerDesign and Preparation of Recombinant Antigens as Diagnostic Reagents in Solid-Phase Immunosorbent AssaysAlan Warnes, Anthony R. Fooks, and John R. StephensonBasic Problems of Serological Laboratory DiagnosisWalter FierzMolecular Diagnostics Resources on the InternetLarry WingerPart VIII. Recombinant Receptor MoleculesCloning Single-Chain Antibody Fragments (scFv) from Hybridoma CellsLars Toleikis, Olaf Broders, and Stefan DübelIndex

Editorial Reviews

"The comprehensive nature of the book makes it an invaluable tool for industry and academia for managing the large amount of data generated in bioinformatics. Each chapter is written in the usual scientific paper format of an abstract, introduction, materials, methods, notes or conclusions and references. The index is well done and makes the book particularly helpful as a reference manual. "-Doody's Health Sciences Book Review Journal"...Anyone who has the interest and resources to develop novel assays not only for infectious diseases but also for other diseases will benefit tremendously from this book." -Archives of Pathology and Laboratory Medicine "...a valuable methodological book for molecular biologists in a hospital laboratory or in academia." -Trends in Parasitology"...a powerful tool for developing better experiments in the diagnostics of infectious diseases." -International Microbiology"...a very good compilation of techniques that can be used for numerous applications." -Analytical Biochemistry"...a useful reference source for anyone who wants to manipulate microbial protein for diagnostic purposes." -International Journal of Medical Microbiology"...this book continues the well-established format of this series from Humana Press in providing clearly described laboratory protocols with useful background discussion...recommended as a useful laboratory reference book..." - Proteomics"The comprehensive nature of the book makes it an invaluable tool for industry and academia for managing the large amount of data generated in bioinformatics." -Doody's Health Sciences and Book Review Journal"...well-organized...provides detailed methods and practical tips...the book can be recommended for students, graduate students, and postdoctoral, fellows in the field of proteomics..." - Clinical Infectious Diseases"...brings together the key elements of oral medicine and nutrition in an accessible form for the dental excellent reference text...wholeheartedly recommend(ed)." - American Society for Clinical NutritionReview of the first edition:"This work represents one of the most up-to-date molecular biology protocols books to date. . .Anyone who has the interest and resources to develop novel assays not only for infectious diseases but also for other diseases will benefit tremendously from this book. . a valuable purchase to keep as a quick protocol source." -Archives of Pathology and Laboratory Medicine