Molecular Genetics of Yeast: A Practical Approach

Paperback | June 1, 1994

EditorJohn R. Johnston

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This book describes and discusses experimental procedures used in a wide range of specialized topics within the genetics and molecular biology of the yeast Saccharomyces cerevisiae. Contributions are by internationally-recognized experts in particular areas and represent up-to-date methodscurrently in use. Topics covered include DNA isolation, cloning, and expression vectors, cosmid cloning, construction and use of cDNA libraries, plasmid shuffling and mutant isolation, Ty insertional mutagenesis, high efficiency transformation, cell-free translation of mRNAs, Ty virus-likeparticles, and applications to industrial strains of yeast. Detailed protocols are clearly presented, with many additional practical tips, to assist both first-time and more experienced experimentalists.

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From the Publisher

This book describes and discusses experimental procedures used in a wide range of specialized topics within the genetics and molecular biology of the yeast Saccharomyces cerevisiae. Contributions are by internationally-recognized experts in particular areas and represent up-to-date methodscurrently in use. Topics covered include DNA is...

John R. Johnston is at University of Strathclyde.

other books by John R. Johnston

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Format:PaperbackDimensions:300 pages, 9.21 × 6.14 × 0.67 inPublished:June 1, 1994Publisher:Oxford University Press

The following ISBNs are associated with this title:

ISBN - 10:0199634297

ISBN - 13:9780199634293

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Table of Contents

1. Procedures for isolating yeast DNA for different purposes2. Construction of cloning and expression vectors3. Cosmid cloning of yeast DNA4. The construction and use of cDNA libraries for genetic selections5. Pulsed field gel electrophoresis6. Plasmid shuffling and mutant isolation7. Ty insertional mutagenesis8. High efficiency transformation with lithium acetate9. Measurement of transcription10. Measurement of mRNA stability11. Production of foreign proteins at high level12. Cell-free translation of natural and synthetic mRNAs13. Virus-like particles: Ty retrotransposons14. The K1 killer toxin: molecular and genetic applications to secretion and cell surface assembly15. Immuno-electron microscopy16. Industrial Saccharomyces yeasts

Editorial Reviews

`The distinguished collection of authors are often pioneers in their subject area and so the guidance is both authoritative and helpful. Overall this is a useful addition to the series, with ready access to standard lab protocols.'Alan Wheals, Society for General Microbiology, November 1996