Molecular Methods for Virus Detection

Other | February 8, 1995

byDanny L. Wiedbrauk, Danny L. Wiedbrauk, Daniel H. Farkas

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Molecular diagnostic procedures have been described in a number of recent books and articles. However, these publications have not focused on virus detection, nor have they provided practical protocols for the newer molecular methods.
Written by the inventors or principal developers of these technologies,Molecular Methods for Virus Detectionprovides both reviews of individual methods and instructions for detecting virus nucleic acid sequences in clinical specimens. Each procedure includes quality assurance protocols that are often ignored by other methodology books.Molecular Methods for Virus Detectionprovides clinically relevant procedures for many of the newer diagnostic methodologies.

Key Features
* Provides state-of-the-art PCR methods for amplification, quantitation,in situhybridization, and multiplex reactions
* Goes beyond PCR with protocols for 3SR, NASBA, LCR, SDA, and LAT
* Covers important virus detection methods such asin situhybridization; Southern, dot, and slot blots; branched chain signal amplification; and chemiluminescence
* Includes quality control information crucial in research and clinical laboratories
* Most chapters are written by the inventors and principal developers of the methodologies
* Includes color plates, 77 figures, and 18 tables

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From the Publisher

Molecular diagnostic procedures have been described in a number of recent books and articles. However, these publications have not focused on virus detection, nor have they provided practical protocols for the newer molecular methods.Written by the inventors or principal developers of these technologies,Molecular Methods for Virus Dete...

Format:OtherDimensions:386 pages, 1 × 1 × 1 inPublished:February 8, 1995Publisher:Elsevier ScienceLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:0080536891

ISBN - 13:9780080536897

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Extra Content

Table of Contents

D.L. Wiedbrauk and A.M. Drevon, Nucleic Acid Detection Methods.
D.L. Wiedbrauk and J. Stoerker, Quality Assurance in the Molecular Biology Laboratory.
D.L. Stoler and N.L. Michael, Nucleic Acid Blotting Techniques for Virus Detection.
J.Carr, In SituHybridization.
R.L. Hodinka, Antiviral Susceptibility Testing Using DNA-DNA Hybridization.
J.C. Wilbur and M.S. Urdea, Quantitation of Viral Nucleic Acids Using Branched DNA (bDNA) Signal Amplification.
I. Bronstein and C.E.M. Olesen, Chemiluminescence Methods.
B.J. McCreedy, Polymerase Chain Reaction.
F. Ferre, P. Pezzoli, E. Buxton, C. Duffy, A. Marchese, and A. Diagle, Quantitation of RNA Targets Using the Polymerase Chain Reaction.
J.B. Mahony and M.A. Chernesky, Multiplex Polymerase Chain Reaction (M-PCR).
G.J. Nuovo, PCRin SituHybridization.
R. Sooknanan, B. van Gemen, and L.T. Malek, Nucleic Acid Sequence Based Amplification (NASBA(tm)).
S.S.Ghosh, E. Fahy, and T.R. Gingeras, The Self-Sustained Sequence Replication (3SR) Reaction and its Application in Clinical Diagnostics and Molecular Biology.
J.D. Burczak, S. Ching, H.-Y. Hu, and H.H. Lee, Ligase Chain Reaction (LCR) for the Detection of Infectious Agents.
G. T. Walker, et al., A Chemiluminescent DNA Probe Test Based upon Strand Displacement Amplification (SDA).
D.M. Schuster, M.S. Berninger, and A. Rashtchian, Ligation Activated Transcription Amplification: Amplification and Detection of Human Papillomaviruses.
Subject Index.