Molecular Pathology Protocols by Anthony A. KilleenMolecular Pathology Protocols by Anthony A. Killeen

Molecular Pathology Protocols

EditorAnthony A. Killeen

Paperback | November 9, 2010

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Anthony Killeen has assembled a collection of readily reproducible molecular pathology techniques that are either frequently performed or recognized for their significant diagnostic utility. Each method is described in step-by-step detail by a leading molecular pathologist or laboratory scientist who has developed it or used it extensively. These clinical laboratory techniques can be used for the diagnosis or monitoring of cancer, hematological malignancies, infectious diseases, and selected genetic disorders. Comprehensive and path-breaking, Molecular Pathology Protocols will enable clinical laboratories to introduce new molecular pathology tests and lay the groundwork for a much-needed standardization in this rapidly developing field.

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Title:Molecular Pathology ProtocolsFormat:PaperbackDimensions:508 pagesPublished:November 9, 2010Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617371319

ISBN - 13:9781617371318

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Table of Contents

DNA Extraction from Paraffin-Embedded TissuesHongxin Fan and Margaret L. GulleyDNA Extraction from Fresh or Frozen TissuesHongxin Fan and Margaret L. GulleyRNA Extraction from Fresh or Frozen TissuesHongxin Fan and Margaret L. GulleySingle-Strand Conformation Polymorphism Analysis of Mutations in Exons 4-8 of the TP53 GeneKirsi H. Vähäkangas, Katariina Castrén, and Judith A. WelshCleavase® Fragment Length Polymorphism Analysis for Genotyping and Mutation DetectionLaura Heisler and Chao-Hung LeeDetection of Telomerase by In Situ Hybridization and by the Polymerase Chain Reaction-Based Telomerase Activity AssayCarmela P. Morales and Shawn E. HoltDetection of Microsatellite InstabilityKarin D. Berg, Constance A. Griffin, and James R. EshlemanPolymerase Chain Reaction Clonality Assays Based on X-Linked GenesLangxing Pan and Huaizheng PengFluorescent In Situ Hybridization: Evaluation for Ploidy and Gene AmplificationSusan SheldonHER-2/neu Oncogene Amplification Determined by Fluorescence In Situ Hybridization, Nested Reverse Transcription-Polymerase Chain Reaction Assay to Detect BCR/ablLinda M. WassermanDetection of t(15;17)(q24;q21), inv(16)/t(16;16)(p13;q22), and t(8;21)(q22;q22) Anomalies in Acute Myeloid LeukemiaDavid S. ViswanathaDetection of t(14;18)(q32;q21)-Associated BCL-2/JH Gene Fusion in Non-Hodgkin's LymphomaDavid S. ViswanathaDetection of Breast Cancer Cells Using Immunomagnetic Beads and Reverse Transcription-Polymerase Chain ReactionScott Luke and Karen L. KaulMolecular Detection of Circulating Prostate Cancer CellsKaren L. KaulMethods to Detect Clonal Gene Rearrangements in Lymphomas and LeukemiasNaheed Mitha and Ronald C. McGlennenMonitoring of Bone Marrow Transplant EngraftmentKristine P. Woronzoff-Dashkoff and Ronald C. McGlennenDirect Molecular Diagnosis of Multiple Endocrine Neoplasia Type 1Elizabeth M. Petty, Michael Glynn, and Allen E. BaleMolecular Detection of Multiple Endocrine Neoplasia Type 2Edward H. Rowsell and Myra J. WickAssay for Detecting the I1307K Susceptibility Allele within the Adenomatous Polyposis Coli GeneStephen B. GruberDetection of Human Papillomaviruses by Polymerase Chain Reaction and In Situ HybridizationElizabeth R. Unger and Suzanne D. VernonMolecular Methods for Detecting Epstein-Barr Virus (Part I): Hybridization to Epstein-Barr Virus-Encoded RNA (EBER) TranscriptsHongxin Fan and Margaret L. GulleyMolecular Methods for Detecting Epstein-Barr Virus (Part II): Structural Analysis of Epstein-Barr Virus DNA as a Marker of ClonalityHongxin Fan and Margaret L. GulleyMolecular Methods for Detecting Epstein-Barr Virus (Part III): EBV Viral Load by Competitive Polymerase Chain ReactionHongxin Fan and Margaret L. GulleyMolecular Detection of Kaposi's Sarcoma-Associated Herpesvirus/Human Herpesvirus-8Ethel CesarmanDiagnostic Applications of Quantitative Polymerase Chain Reaction for Cytomegalovirus, Polymerase Chain Reaction System That Detects Herpes Simplex Virus in Cerebrospinal Fluid and Discriminates Genotypes 1 and 2Yi-Wei TangDetection and Typing of Hepatitis C VirusFrederick S. NolteDetection and Speciation of Mycobacteria in Formalin-Fixed, Paraffin-Embedded Tissue SectionsDiana Mohl and Thomas J. GiordanoUltrasensitive Quantitation of Human Immunodeficiency Virus Type 1 RNA in Plasma by the AMPLICOR and COBAS AMPLICOR HIV-1 MONITORTM TestsSteven Herman, James Novotny, and Maurice RosenstrausMolecular Diagnosis of Hereditary Thrombotic DisordersJames G. DonnellyPrenatal Genotyping of the RhD Locus to Identify Fetuses at Risk for Hemolytic Disease of the NewbornMartin J. Hessner and Daniel B. BellissimoMolecular Diagnosis of Hereditary HemochromatosisSuzanne Huber and Karl V. VoelkerdingGenotyping of Apolipoprotein EI. Cetin Ozturk, Nahida Akel, and Anthony A. KilleenGenotyping for Functionally Important Human CYP2D6*4 (B) Mutation Using TaqMan ProbesMichael M. Shi, Scott P. Myrand, Michael R. Bleavins, and Felix A. de la IglesiaIndex

Editorial Reviews

"It contains cutting-edge molecular diagnostic techniques with detailed step-by-step instructions and tips to obtain successful results....the purpose here is to offer readily reproducible molecular pathology techniques that are either frequently performed or recognized for their significant diagnostic utility. It is a worthy objective and the editor meets it very well....Certain methodological details are described in the notes, which provide much helpful information in methodology and tips on how to avoid pitfalls. This form of presentation is attractive both to newcomers to the field and to those with some experience in diagnostic molecular pathology....Cutting-edge methods include telomerase, microsatellite instability, chromosomal translocations, and fluorescent in situ hybridization assays....The significant diagnostic utility of molecular protocols in cancer, hermatological malignancies, and virus related diseases is covered well....It is a good protocol resource book in molecular pathology."-Doody's Health Sciences Book Review Journal"Molecular Pathology Protocols fills a unique niche for general molecular pathology laboratories that may be called upon to perform a wide variety of tests for infectious and noninfectious diseases. It has 35 chapters that provide extensive detail on laboratory protocols for a variety of important conditions, from techniques for preparation of nucleic acid templates and mutation detection for genetic and neoplastic evaluations, to hematological applications, to a limited number of detailed protocols for infectious disease diagnosis and monitoring...The major strength of the protocols discussed in this book lies in their enormous detail and the practical remarks of the authors, which will assist in establishing the assays in a new laboratory. Another great value in some chapters is the emphasis on the assessment of quality control that will meet or exceed all the requirements of agencies that license and regulate clinical laboratories." - Clinical Infectious Diseases"A must for genetic laboratories and all those using the new molecular biological methods." - Journal of Endocrine Genetics"Each protocol in this book includes sections of introduction, materials, methods, and notes. Certain methodological details are described in the notes, which provide much helpful information in methodology and tips on how to avoid pitfalls. This form of presentation is attractive both to newcomers to the field and to those with some experience in diagnostic molecular pathology. Most protocols use polymerase chain reaction (PCR) based techniques. Cutting-edge methods include telomerase, microsatellite instability, chromosomal translocations, and fluorescent in sity hybridization assays. This book has good references in both background of diseases and techniques. Most charts and labels have high quality;...The significant diagnostic utility of molecular protocols in cancer, hematological malignancies, and virus related diseases is covered well....This book is well written and the molecular protocols introduced can be easily followed practically." - E-Streams