Molecular Plant Biology Volume 2: A Practical Approach

Paperback | August 7, 2003

EditorPhilip M Gilmartin, Chris Bowler

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Volume Two focuses on experimental approaches for studies on gene expression, gene product analysis, with the final section devoted to emerging technologies. Topics covered include a range of techniques for transcript analysis, including In situ Hybridization and DNA microarrays. DNA-proteininteraction methods are also covered in detail. Inducible gene expression in plants as well as expression and analysis of recombinant proteins, and analysis of protein import into chloroplasts are covered as well as techniques for fractionation of plant tissue for biochemical analyses and the studyof protein-protein interactions with the yeast two-hybrid system. A range of approaches for using antibodies as tools are also described including the use of antibody phage display libraries. The final section on emerging technologies describes methodologies for calcium imaging and for the spatialand temporal analysis of reporter genes such as luciferase and green fluorescent protein. The final area covers a range of experimental procedures for moss, which is emerging as a new model organism.

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Volume Two focuses on experimental approaches for studies on gene expression, gene product analysis, with the final section devoted to emerging technologies. Topics covered include a range of techniques for transcript analysis, including In situ Hybridization and DNA microarrays. DNA-proteininteraction methods are also covered in detai...

Philip M Gilmartin is Director, Centre for Plant Sciences, University of Leeds. Chris Bowler is Head, Laboratory of Molecular Plant Biology, Stazione Zoologica, Napoli, Italy.

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Format:PaperbackDimensions:9.69 × 7.44 × 0.78 inPublished:August 7, 2003Publisher:Oxford University PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:0199638187

ISBN - 13:9780199638185

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Table of Contents

Part 1: Gene Expression1. Transcript analysis2. In situ hybridization3. DNA microarrays for gene expression analysis4. DNA-protein interactions5. Inducible gene expression in plantsPart 2: Gene Product Analysis6. Expression of recombinant proteins7. Import of proteins into isolated chloroplasts and thylakoid membranes8. Fractionation of plant tissue for biochemical analysis9. Studying protein-protein interactions with the yeast two-hybrid system10. Antibody techniques11. Antibody phage display librariesPart 3: Emerging Technologies12. Spatial and temporal measurements of calcium ions in living plant cells13. Reporter genes for plant cells14. Moss gene technology