Molecular Toxicology Protocols by Phouthone KeohavongMolecular Toxicology Protocols by Phouthone Keohavong

Molecular Toxicology Protocols

EditorPhouthone Keohavong

Paperback | September 21, 2011

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A collection of cutting-edge techniques for analyzing genotoxic exposure and detecting the resulting biological effects-including endogenous metabolites-up to and including the development of cancer. The authors emphasize analytical methods that can be specifically applied to human populations and patients. Among the applications detailed are the analysis of interactions between such cellular macromolecules as DNA and proteins and chemical and physical agents, the assessment of medically relevant toxicity, and the characterization of genetic alterations induced in transgenic animals by in vivo systems. There are also methods for the analysis of genotoxic exposure during gene expression, of cytotoxicity caused by the induction of apoptosis, of genetic alterations in reporter genes and oncogenes, early (premalignant) detection of altered oncogenes, and of individual variation in biotransformation and DNA repair capacity.
Title:Molecular Toxicology ProtocolsFormat:PaperbackDimensions:504 pagesPublished:September 21, 2011Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617373478

ISBN - 13:9781617373473

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Table of Contents

Part I. Analysis of DNA Adducts32P-Postlabeling Analysis of DNA AdductsDavid H. Phillips, Alan Hewer, and Volker M. ArltModification of the 32P-Postlabeling Method to Detect a Single Adduct Species as a Single SpotMasako Ochiai, Takashi Sugimura, and Minako NagaoDNA Isolation and Sample Preparation for Quantification of Adduct Levels by Accelerator Mass SpectrometryKaren H. Dingley, Esther A. Ubick, John S. Vogel, and Kurt W. HaackFluoroimaging-Based Immunoassay of DNA Photoproducts in Ultraviolet-B-Irradiated TadpolesIovanna Pandelova, Stephen R. Hewitt, and John B. HaysAnalysis of DNA Strand Cleavage at Abasic SitesWalter A. Deutsch and Vijay HegdePart II. Detection of Chromosomal and Genome-Wide DamagePremature Chromosome Condensation in Human Resting Peripheral Blood Lymphocytes for Chromosome Aberration Analysis Using Specific Whole-Chromosome DNA Hybridization ProbesPataje G. S. Prasanna and William F. BlakelyMutagen-Induced Chromatid Breakage as a Marker of Cancer RiskXifeng Wu, Yun-Ling Zheng, and T. C. HsuFlow Cytometric Analysis of Micronuclei in Erythrocytes, Jan GrawéThe Comet Assay: A Sensitive Genotoxicity Test for the Detection of DNA DamageGünter Speit and Andreas HartmannComputerized Image Analysis Software for the Comet AssayR. C. ChaubeyThe Comet-FISH Technique: A Tool for Detection of Specific DNA Damage and RepairAlexander Rapp, Michael Hausmann, and Karl Otto GreulichDNA Double-Strand Break Damage and Repair Assessed by Pulsed-Field Gel ElectrophoresisNina Joshi and Stephen G. GrantPart III. Detection and Characterization of Surrogate Gene MutationAnalysis of In Vivo Mutation in the Hprt and Tk Genes of Mouse LymphocytesVasily N. Dobrovolsky, Joseph G. Shaddock, and Robert H. HeflichQuantifying In Vivo Somatic Mutations Using Transgenic Mouse Model SystemsRoy R. SwigerMethods for Detecting Somatic Mutations In Vitro: The Human T-Cell Cloning Assay Selecting for HPRT MutantsSai-Mei HouMolecular Analysis of Mutations in the Human HPRT GenePhouthone Keohavong, Liqiang Xi, and Stephen G. GrantSimultaneous Quantification of t(14;18) and HPRT Exon 2/3 Deletions in Human LymphocytesJames C. FuscoeThe GPA In Vivo Somatic Mutation AssayStephen G. GrantFlow Cytometric Measurement of Mutant T Cells With Altered Expression of TCR: Detecting Somatic Mutations in Humans and MiceSeishi Kyoizumi, Yoichiro Kusunoki, and Tomonori HayashiPart IV. Detection and Characterization of Cancer Gene MutationMutation Screening of the TP53 Gene by Temporal Temperature Gradient Gel ElectrophoresisTherese Sørlie, Hilde Johnsen, Phuong Vu, Guro Elisabeth Lind, Ragnhild Lothe, and Anne-Lise Børresen-DaleAnalysis of K-RAS and P53 Mutations in Sputum SamplesWeimin Gao and Phouthone KeohavongAllele-Specific Competitive Blocker-PCR Detection of Rare Base SubstitutionBarbara L. Parsons, Page B. McKinzie, and Robert H. HeflichGel-Based Nonradioactive Single-Strand Conformational Polymorphism and Mutation Detection: Limitations and SolutionsVibhuti Gupta, Reetakshi Arora, Anand Ranjan, Narendra K. Bairwa, Dheeraj K. Malhotra, P. T. Udhayasuriyan, Anjana Saha, and Ramesh BamezaiDetection and Characterization of Oncogene Mutations in Preneoplastic and Early Neoplastic LesionsToshinari MinamotoDetection of DNA Double-Strand Breaks and Chromosome Translocations Using Ligation-Mediated PCR and Inverse PCRMichael J. Villalobos, Christopher J. Betti, and Andrew T. M. VaughanPart V. Analysis of DNA Repair MechanismsMicrosatellite Instability: An Indirect Assay to Detect Defects in the Cellular Mismatch Repair MachineryAnjana Saha, Narendra K. Bairwa, and Ramesh BamezaiUnscheduled DNA Synthesis: A Functional Assay for Global Genomic Nucleotide Excision RepairCrystal M. Kelly and Jean J. LatimerAnalysis of DNA Repair Using Transfection-Based Host Cell ReactivationJennifer M. Johnson and Jean J. LatimerAn Immunoassay for Measuring Repair of Ultraviolet PhotoproductsShirley McCreadyAnalysis of DNA Double-Strand Break Repair by Nonhomologous End Joining in Cell-Free Extracts From Mammalian CellsPetra Pfeiffer, Elke Feldmann, Andrea Odersky, Steffi Kuhfittig-Kulle, and Wolfgang GoedeckeMeasuring Recombination Proficiency in Mouse Embryonic Stem CellsAndrew J. Pierce and Maria JasinPart VI. Array TechnologiesStrategies for Measurement of Biotransformation Enzyme Gene ExpressionMarjorie Romkes and Shama C. BuchGenotyping Technologies: Application to Biotransformation Enzyme Genetic Polymorphism ScreeningMarjorie Romkes and Shama C. BuchTaqMantm Fluorogenic Detection System to Analyze Gene Transcription in Autopsy MaterialKaori Shintani-Ishida, Bao-Li Zhu, and Hitoshi MaedaDevelopment of Quantitative Reverse Transcriptase PCR Assays for Measuring Gene ExpressionTony E. Godfrey and Lori A. KellyPart VII. ApoptosisQuantification of Selective Phosphatidylserine Oxidation During ApoptosisJames P. Fabisiak, Yulia Y. Tyurina, Vladimir A. Tyurin, and Valerian E. KaganQuantitative Method of Measuring Phosphatidylserine Externalization During Apoptosis Using Electron Paramagnetic Resonance Spectroscopy and Annexin-Conjugated IronJames P. Fabisiak, Grigory G. Borisenko, and Valerian E. KaganDetection of Programmed Cell Death in Cells Exposed to Genotoxic Agents Using a Caspase Activation AssayMichael E. Gehring and Patrick P. KotyIndex

Editorial Reviews

"This book should be of interest to both specialist researchers as well as members of the wider scientific community..." - British Journal of Anesthesiology