Monoclonal Antibodies: A Practical Approach by Phil S. Shepherd

Monoclonal Antibodies: A Practical Approach

EditorPhil S. Shepherd, Christopher Dean

Paperback | April 15, 2000

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Monoclonal Antibodies: A Practical Approach covers the preparation, testing, derivation, and applications of monoclonal antibodies. New immunological techniques incorporating tried and tested methodologies are described, making the book of interest to established and inexperiencedimmunologists. Both the standard somatic hybridization technique and recombinant techniques, including the use of phage libraries, for the preparation of rodent and human monoclonal antibodies are described. Protocols for both the small and large scale production are detailed, as well aspurification and labelling (with both radioisotopes and non-radioisotopes) methods. The applications of monoclonal antibodies in immunoblotting, enzyme linked immunoassays, immunofluorescence, and FACS analysis are all covered in detail. Finally protocols are given for the use of monoclonalantibodies in rheumatoid arthritis, tissue typing, detecting DNA modified during chemotherapy, and in the clinical analysis of transplantation samples for malignancy. This book will therefore be an invaluable laboratory companion to anyone using monoclonal antibodies in their research.

About The Author

Phil S. Shepherd is at The Guy's, King's College, and St Thomas's Hospitals, London.

Details & Specs

Title:Monoclonal Antibodies: A Practical ApproachFormat:PaperbackPublished:April 15, 2000Publisher:Oxford University PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:0199637229

ISBN - 13:9780199637225

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Table of Contents

Preparation of Rodent Monoclonal Antibodies by in vitro Somatic HybridizationPreparation of Recombinant Antibodies From Immune Rodent Spleens and the Design of Their Humanisation by CDR GraftingSelection of Antibodies From Phage Libraries of Immunoglobulin GenesArm Complexes for in vitro Display and Evolution of Antibody Combining SitesHuman Monoclonal Antibodies to Blood Group AntigensLaboratory Based Methods for Small-Scale Productions of Monoclonal AntibodiesIsolation and Purification of Antibodies From Tissue Culture SupernatantAntibody Production in PlantsRadiolabelling of Monoclonal AntibodiesNon-Radioactive Antibody ProbesImmunogold Probes for Light and Electron MicroscopyCharacterization of Cellular Antigens Using Monoclonal AntibodiesImmunoassaysImmunoaffinity Chromatography of MacromoleculesImmunochemical Detection of BrdUrd-Labelled Nuclei for Monitoring Cell KineticsImmunofluorescence MicroscopyAnalysis of Clinical Haematological Samples in Transplantation for CancerImmunocytochemical Staining of Cells and Tissues for Diagnostic ApplicationsDetection of Chemically Modified DNA in Lymphocytes of Patients Undergoing ChemotheraphyMonoclonal Antibody Therapy in Organ TransplantationMonoclonal Antibody Therapy in Rheumatoid Arthritis