Monoclonal Antibodies: Methods and Protocols by Maher AlbitarMonoclonal Antibodies: Methods and Protocols by Maher Albitar

Monoclonal Antibodies: Methods and Protocols

EditorMaher Albitar

Paperback | November 19, 2010

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This book examines a collection of state-of-the-art methods that employ monoclonal antibodies in a clinical setting. The chapters offer in-depth description for generating mouse and recombinant humanized antibodies, and a comprehensive review of how antibodies are being used in bead-based methods for measuring proteins. This field will continue to expand and provide new and innovative techniques in the laboratory and as a basis that complements targeted therapy.
Title:Monoclonal Antibodies: Methods and ProtocolsFormat:PaperbackDimensions:240 pages, 9.02 × 5.98 × 0 inPublished:November 19, 2010Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617376469

ISBN - 13:9781617376467

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Table of Contents

Table of Contents1. Development and Characterization of Mouse Hybridomas.2. Recombinant Monoclonal Antibodies.3. Soluble Fc Fusion Proteins for Biomedical Research.4. The use of the Antibodies in the Diagnosis of Leukemia and Lymphoma by Flow Cytometry.5. Quantification of Surface Antigens and Quantitative Flow Cytometry.6. CD13 and CD10 expression of granulocytes as markers for the functioning of the immune system. Quantification of the expression of membrane molecules using 1:1 labeled monoclonal antibodies and flow cytometry.7. Monitoring Cell Signaling pathways by Quantitative Flow Cytometry.8. Antibodies and Immunohistochemical Evaluation for the Diagnosis of Hematologic Malignancies: An Overview 9. Bead Based Multi-Analyte Flow Immunoassays: The Cytometric Bead Array (CBA) System.10. Cell-Free bead-based Detection of Total and Phosphorylated Proteins in Plasma and Cell Lysate.11. Measuring Humanized Antibodies in Plasma of Patients Treated with Antibody-based Therapy using Bead-based Flow Cytometry.12. Detection of Chromosome Translocations by Bead-based Flow Cytometry.13. Detection of the MDR1 P-Glycoprotein expression and function.14. Detection of human antibodies generated against therapeutic antibodies used in tumor therapy.15. Measuring Allergen-Specific IgE: Where have we been and where are we going?

Editorial Reviews

"This is the book for you if you are interested in implementing on eor more of these assays in your laboratory." -Doody's Book Review, Weighted Numerical Score:80 - 3 Stars