Monoclonal Antibodies: Principles and Practice

Hardcover | March 5, 1996

byJames W. GodingEditorJames W. Goding

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Monoclonal Antibodies now have applications in virtually all areas of biology and medicine, and much of the world's biotechnology industry has its foundations in the exploitation of this technology.The Third Edition of this well established book meets the needs of both newcomers to the area and experienced researchers, by providing an integrated treatment of both the production and application of monoclonal antibodies. As in previous editions, detailed and critical accounts of the theory, production, purification, fragmentation, storage and radiolabelling of monoclonal antibodies are given, along with descriptions of their use in antigen characterization, affinity chromatography and immunofluorescence. The present volume has been comprehensively updated to cover recent rapid advances, particularly with respect to the applications of molecular biology, the use of antibodies in closing and heterologous expression of genes, immunohistology and phage display libraries. Since the previous edition, there has been a growing trend towards the replacement of procedures using radioactive isotopes, and the current edition incorporates these newer technologies.The text is oriented towards problems solving, and makes it easy to adapt each procedure to individual needs. Extensive cross-referencing, a glossary and a comprehensive index make this book an essential reference.This book will be vital both for laboratories already producing or using monoclonal antibodies, and for workers in many disciplines who are contemplating their use. * * Provides an integrated treatment of both the production and application of monoclonals in cell biology, biochemistry, and immunology.* Gives detailed and critical accounts of the theory, production, purification, storage, and relabelling of monoclonals, and their use in antigen characterization, affinity chromorography, and immunofluroscence.* Comprehensively updated to cover the rapid advances that have occurred since the publication of the Second Edition.

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From Our Editors

Monoclonal antibodies now have applications in virtually all areas of biology and medicine, and much of the world's biotechnology industry has its foundations in the exploitation of this technology.

From the Publisher

Monoclonal Antibodies now have applications in virtually all areas of biology and medicine, and much of the world's biotechnology industry has its foundations in the exploitation of this technology.The Third Edition of this well established book meets the needs of both newcomers to the area and experienced researchers, by providing an ...

From the Jacket

Monoclonal Antibodies now have applications in virtually all areas of biology and medicine, and much of the world's biotechnology industry has its foundations in the exploitation of this technology.The third edition of this well established book meets the needs of both newcomers to the area and experienced researchers, by providing an ...

Format:HardcoverDimensions:492 pages, 9 × 6 × 0.98 inPublished:March 5, 1996Publisher:Academic PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:0122870239

ISBN - 13:9780122870231

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Table of Contents

Introduction. The Antibody Response. Cellular Basis of the Immune System. Nature of Antigens. Antibody Structure and Function. Genetics of Antibodies. Introduction to Monoclonal Antibodies. Production of Monoclonal Antibodies. Purification. Fragmentation and Isotopic Labeling of Monoclonal Antibodies. Analysis of Antigens Recognized By Monoclonal Antibodies. Affinity Chromatography Immunofluorescence. Immunohistology. Construction, Screening and Expression of Recombinant Antibodies. Generation of Conventional Antibodies.

From Our Editors

Monoclonal antibodies now have applications in virtually all areas of biology and medicine, and much of the world's biotechnology industry has its foundations in the exploitation of this technology.

Editorial Reviews

Reviews of Previous Editions:"An excellent laboratory manual, invaluable to the newcomer to the field."--NATURE"Lucid, concise yet comprehensive... the author has managed to execute his task with some brilliancy; it is a pleasure to read or consult the book."--FOLIA BIOLOGICA"...for those who are involved in the more general production and use of monoclonal antibodies this is an excellent, comprehensive text."--BR F BIOMED SCI