Morphology Methods: Cell and Molecular Biology Techniques by Ricardo V. LloydMorphology Methods: Cell and Molecular Biology Techniques by Ricardo V. Lloyd

Morphology Methods: Cell and Molecular Biology Techniques

byRicardo V. Lloyd

Paperback | December 9, 2010

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Ricardo Lloyd, md, phd, provides critical and tutorial surveys of the cutting-edge molecular and cell biologic techniques used today in the analysis of cells and tissues in situ. These molecular-based histologic and morphologic techniques are described in step-by-step detail by hands-on experts, often those who have originated or perfected them. Among the techniques presented are laser capture microdissection, in situ hybridization, FISH, confocal laser scanning microscopy, in situ PCR, and tyramide amplification. Applications include the detection of infectious agents and DNA, the diagnosis of undifferentiated tumors and endocrine lesions, the metaphase analysis of whole blood cultures, and clonality analysis. In its integrated, state-of-the-art approach, Morphology Methods: Cell and Molecular Biology Techniques thoroughly details and applies the rich panoply of significant new cell and molecular biologic technologies to all the major problems confronting morphologic studies and diagnostic pathology today.
Title:Morphology Methods: Cell and Molecular Biology TechniquesFormat:PaperbackDimensions:422 pagesPublished:December 9, 2010Publisher:Springer-Verlag/Sci-Tech/TradeLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617372730

ISBN - 13:9781617372735

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Table of Contents

Introduction to Molecular MethodsRicardo V. LloydLaser Capture Microdissection: Principles and ApplicationsRicardo V. Lloyd (Protocols: H&E Staining, Operating the PIX Cell System; DNA Extraction from Collected Cells; RNA Extraction from Collected Cells)In Situ Hybridization: Detection of DNA and RNALong Jin, Xiang Qian, and Ricardo V. Lloyd (Protocol: In Situ Hybridization)In Situ Detection of Infectious AgentsGary W. Procop and Randall Hayden (Protocols: Detecting Adenovirus, Epstein-Barr Virus)Fluorescent In Situ Hybridization (FISH)Susan Sheldon (Protocol: FISH Materials)Practical Applications of the FISH TechniqueGeorge Kontogeorgos, Nikiforos Kapranos, and Eleni Thodou (Protocols: Metaphase Analysis from Whole Blood Culture; Metaphase Analysis from Solid Tumors; Interphase Analysis on Cell Imprints-Cytology Specimens; Interphase Analysis of Formalin-Fixed Paraffin-Embedded Tissues)Tyramide Amplification Methods for In Situ HybridizationJohn L. Frater and Raymond R. Tubbs (Protocol: TSA, 1995; CARD/TSA with Nanogold; Tyramide-enhanced FISH with Peroxidase-labeled Oligonucleotides)Protocols: Ultrastructural In Situ HybridizationAkira Matsuno, Tadashi Nagashima, R. Yoshiyuki Osamura, and Keiichi WatanabeQuantitation of In Situ Hybridization AnalysisLars-Inge LarssonProtocols: Confocal Laser Scanning MicroscopyAkira Matsuno, Johbu Itoh, Tadashi Nagashima, R. Yoshiyuki Osamura, and Keiichi WatanabeProtocols: Polymerase Chain ReactionYuri E. Nikiforov and Philip N. HowlesProtocols: Detection of Nucleic Acids in Cells and Tissues by In Situ Polymerase Chain ReactionOmar Bagasra, Lisa E. Bobroski, and Muhammad AmjadImmunohistochemistry: Theory and PracticePatrick C. Roche and Eric D. HisAntigen Retrieval Technique for Immunohistochemistry: Principles, Protocols, and Further DevelopmentClive R. Taylor and Shan-Rong Shi (Protocol: Antigen Retrieval (AR) Technique)Tyramide Amplification in ImmunohistochemistryNaoko Sanno, Akira Teramoto, and R. Yoshiyuki Osamura (Protocol: CARD Immunohistochemistry)Application of Immunohistochemistry in the Diagnosis of Lymphoid LesionsPaul J. KurtinApplications of Immunohistochemistry in the Diagnosis of Undifferentiated TumorsMark R. Wick and Lisa ACerilli. Applications of Immunohistochemistry in the Diagnosis of Endocrine LesionsRicardo V. LloydUltrastructural ImmunohistochemistrySergio Vidal, Eva Horvath, and Kalman Kovacs (Protocol: Ultrastructural Immunohistochemistry)Protocols: Clonality Analysis,Aurel Perren and Paul Komminoth

Editorial Reviews

"The book nicely covers the hot topics in this area -- laser capture microdissection, in situ hybridization, FISH, tyramide amplification methods, etc. An attractive feature of this book is that the last 10 chapters (of the 20) contain actual "cookbook" protocols (e.g., PCR, confocal laser scanning microscopy, immunohistochemistry, antigen retrieval, etc.) that can be adapted immediately to any laboratory...This book would be useful for those needing a concise reference for how to perform modern molecular tissue-based technology. " -Doody's Health Science Book Review Journal"This book describes in detail new methods employed in the study of molecular morphology as a basis for the study of human genes and the etiology of diseases. Among the methods are in situ hybridization, in situ detection of infectious agents, the "FISH" technique, PCR, immunocytochemistry, etc. This excellent production has many photos, some in color, and tables and figures. Very useful to those wishing to employ these techniques." - Journal of Pediatric Endocrinology and Metabolism