Mouse Models of Allergic Disease: Methods and Protocols by Irving C. AllenMouse Models of Allergic Disease: Methods and Protocols by Irving C. Allen

Mouse Models of Allergic Disease: Methods and Protocols

EditorIrving C. Allen

Hardcover | August 14, 2013

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Over the last half century, a dramatic increase in allergic diseases has been observed throughout industrialized nations, which has resulted in significant worldwide socio-economic challenges. In Mouse Models of Allergic Disease: Methods and Protocols, a wide range of expert contributors provide detailed protocols for the design and execution of experiments to thoroughly analyze critical elements associated with a diverse range of allergic diseases, all through the lens of mouse models that accurately recapitulate clinically relevant aspects of the respective human disease.  The volume opens with a section featuring techniques essential for effective ex vivo cell isolation and evaluation of specific cell types relevant to a diverse range of allergic diseases, and the book then moves on to cover in vivo protocols to evaluate prevalent mouse models of human allergic diseases, including mouse models of systemic anaphylaxis, contact hypersensitivity, allergic rhinitis, and asthma, as well as a collection of chapters on in vivo and ex vivo protocols used to assess indirect mediators of allergic diseases, such as the nervous system, non-hematopoietic cells, and the composition of the gut microbiome.  Written in the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Timely and authoritative, Mouse Models of Allergic Disease: Methods and Protocols serves as an essential collection of protocols that allow both novice and expert researchers the ability to accurately develop, evaluate, and characterize the mechanisms associated with these disorders.
Title:Mouse Models of Allergic Disease: Methods and ProtocolsFormat:HardcoverDimensions:315 pages, 10 × 7.01 × 0.01 inPublished:August 14, 2013Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1627034951

ISBN - 13:9781627034951

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Table of Contents

1. Transgenic Mouse Models            Jennifer Skelton, Linda H. Gower, Anne McGough, and Patricia A. Labosky 2. Pulmonary Antigen Presenting Cells: Isolation, Purification, and Culture            Hideki Nakano and Donald N. Cook 3. Evaluation of T Cell Function in Allergic Disease            Brianne R. Barker 4. Evaluating B-Cells: From Bone Marrow Precursors to Antibody Producing Cells            M. Manuela Rosado, Marco Scarsella, Simona Cascioli, Ezio Giorda, and Rita Carsetti 5. Protocols for Identifying, Enumerating and Assessing Mouse Eosinophils            Kimberly D. Dyer, Katia E. Garcia-Crespo, Caroline M. Percopo, Eva M. Sturm, and Helene F. Rosenberg 6. Evaluation of Classical, Alternative, and Regulatory Functions of Bone Marrow-Derived Macrophages            Beckley K. Davis 7. Applications of Mouse Airway Epithelial Cell Culture for Asthma Research            Amjad Horani, John D. Dickinson, and Steven L. Brody 8. Isolation and Characterization of Mast Cells in Mouse Models of Allergic Diseases            Martina Kovarova 9. Purifying and Measuring Immunoglobulin E (IgE) and Anti-IgE            Jamie L. Sturgill and Daniel H. Conrad 10. Protocols for the Induction and Evaluation of Systemic Anaphylaxis in Mice            Elizabeth Doyle, Julia Trosien, and Martin Metz 11. Contact Hypersensitivity Models in Mice            Irving C. Allen 12. Induction of Allergic Rhinitis in Mice            Virginia McMillan Carr and Alan M. Robinson 13. Induction of Allergic Airway Disease Using House Dust Mite Allergen            Irving C. Allen 14. An Inhalation Model of Allergic Fungal Asthma: Aspergillus fumigatus-Induced Inflammation and Remodeling in Allergic Airways Disease            Jane M. Schuh and Scott A. Hoselton 15. PAMPs and DAMPs in Allergy Exacerbation Models            Monique A.M. Willart, Philippe Poulliot, Bart N. Lambrecht, and Mirjam Kool 16. Assessment of Airway Hyperresponsiveness in Mouse Models of Allergic Lung Disease Using Detailed Measurements of Respiratory Mechanics            John M. Hartneyand Annette Robichaud 17. Bilateral Vagotomy as a Tool for Determining Autonomic Involvement in Airway Responses in Mouse Models of Asthma            Jaime M. Cyphert 18. Clara Epithelial Cell Depletion in the Lung            Sanchaita S. Sonar and Jan C. Dudda 19. A Mouse Model for Evaluating the Contribution of Fibrocytes and Myofibroblasts to Airway Remodeling in Allergic Asthma            Matthias Schmidt and Sabrina Mattoli 20. Assessment of Airway Hyperresponsiveness in Murine Tracheal Rings            Jeremiah T. Herlihy, Iurii Semenov, and Robert Brenner 21. Use of the Cockroach Antigen Model of Acute Asthma to Determine the Immunomodulatory Role of Early Exposure to Gastrointestinal Infection            Carolyn G. Durham, Lisa M. Schwiebert, and Robin G. Lorenz 22. Expression Profiling to Identify Candidate Genes Associated with Allergic Phenotypes            Willie June Brickey 23. Flow Cytometric Methods for the Assessment of Allergic Disease            Adeeb H. Rahman 24. Generation of Bone Marrow and Fetal Liver Chimeric Mice            Eda K. Holl