Mouse Models of Innate Immunity: Methods and Protocols by Irving C. AllenMouse Models of Innate Immunity: Methods and Protocols by Irving C. Allen

Mouse Models of Innate Immunity: Methods and Protocols

EditorIrving C. Allen

Hardcover | July 4, 2013

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The innate immune system represents a critical arm of the immune response by providing immediate and robust host defense; however, human studies of its function are often limited by ethical, logistical, and technical obstacles. In Mouse Models of Innate Immunity: Methods and Protocols, experts in the field explore the design and execution of experiments used to thoroughly evaluate critical elements associated with the host innate immune response. The volume opens with methods that are essential for collecting and assessing various primary cells that are highly relevant to innate immunity, and it continues with in vivo protocols commonly used to evaluate the innate immune response in the mouse, including mouse models of respiratory infection, gastrointestinal inflammation, fungal and parasitic diseases, sepsis, and HIV-1 infection.  Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and easy to use, Mouse Models of Innate Immunity: Methods and Protocols will serve the research community by providing expert advice and protocols that allow both experienced and novice investigators to successfully plan, implement, and assess disease processes associated with the innate immune response.

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Title:Mouse Models of Innate Immunity: Methods and ProtocolsFormat:HardcoverDimensions:270 pages, 10 × 7.01 × 0 inPublished:July 4, 2013Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1627034803

ISBN - 13:9781627034807

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Table of Contents

1. Conventional Murine Gene Targeting            Albert G. Zimmermann and Yue Sun 2. Production and Characterization of Humanized Rag2-/-?c-/- Mice            Freddy M. Sanchez, German I. Cuadra, Stanton J. Nielsen, Anne Tanner, and Bradford K. Berges 3. Isolation, Culture, and Functional Evaluation of Bone Marrow-Derived Macrophages            Beckley K. Davis 4. Collecting Resident or Thioglycollate Elicited Peritoneal Macrophages            Monika Schneider 5. Quantification and Visualization of Neutrophil Extracellular Traps (NETs) from Murine Bone Marrow-Derived Neutrophils            Linda Vong, Philip M Sherman, and Michael Glogauer 6. Assessment of Oxidative Metabolism            Emilie Imbeault and Denis Gris 7. Generation and Culture of Mouse Embryonic Fibroblasts            Yu Lei 8. Primary Ear Fibroblast Derivation from Mice            Chris B. Moore and Irving C. Allen 9. Bone Marrow Derived Dendritic Cells            Kelly Roney 10. Measuring T Cell Function in Innate Immune Models            Brianne R. Barker 11. Bioassay for the Measurement of Type-I Interferon Activity            Douglas G. Widman 12. Safe and Effective Mouse Foot-Pad Inoculation            Kristin M. Long and Mark Heise 13. Delayed Type Hypersensitivity Models in Mice            Irving C. Allen 14. Mouse Model of Staphylococcus aureus Skin Infection            Natalia Malachowa, Scott D. Kobayashi, Kevin R. Braughton, and Frank R. DeLeo 15. Sepsis Induced by Cecal Ligation and Puncture            Haitao Wen 16. Systemic Infection of Mice with Listeria monocytogenes to Characterize Host Immune Responses            Nancy Wang, Richard A. Strugnell, Odilia L. Wijburg, and Thomas C. Brodnicki 17. Mouse Model of Invasive Fungal Infection            Donna M. MacCallum 18. Endotoxin-Induced Uveitis in Rodents            Umesh C.S. Yadav and Kota V. Ramana 19. Bacteria-Mediated Acute Lung Inflammation            Irving C. Allen 20. Intranasal Influenza Infection of Mice and Methods to Evaluate Progression and Outcome            Catherine J. Sanders, Brian Johnson, Charles W. Frevert, and Paul G. Thomas 21. Dextran Sodium Sulfate-Induced Murine Inflammatory Colitis Model            Monika Schneider 22. Bacterial-Mediated Gastrointestinal Inflammation            Joshua Uronis and Xiaolun Sun 23. Plasmodium berghei ANKA (PbA) Infection of C57BL/6J Mice: A Model of Severe Malaria            Marcela Montes de Oca and Ashraful Haque 24. Characterization of HIV-1 Infection in the Humanized Rag2-/-?c-/- Mouse Model            Freddy M. Sanchez and Bradford K. Berges