Multiple Myeloma: Methods and Protocols by Ross D. BrownMultiple Myeloma: Methods and Protocols by Ross D. Brown

Multiple Myeloma: Methods and Protocols

EditorRoss D. Brown, P. Joy Ho

Paperback | November 10, 2010

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A comprehensive collection of classic and innovative methodologies used in many laboratories for the investigation of multiple myeloma. These readily reproducible techniques range from the standard Plasma Cell Labeling Index methodology to a final chapter on making sense of microarrays, and include the full spectrum of cytogenetic and molecular diagnostic methods. The protocols follow the successful Methods in Molecular MedicineT series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. These proven techniques are ideal for studying the pathogenesis of multiple myeloma and identifying new therapeutic targets.
Title:Multiple Myeloma: Methods and ProtocolsFormat:PaperbackDimensions:324 pagesPublished:November 10, 2010Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617375373

ISBN - 13:9781617375378

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Table of Contents

Multiple Myeloma: Challenges and OpportunitiesDouglas Joshua, Ross D. Brown, and P. Joy HoImmunophenotyping of Plasma Cells in Multiple MyelomaGema Mateo Manzanera, Jesús F. San Miguel Izquierdo, and Alberto Orfao de MatosPlasma Cell Labeling IndexPhilip R. Greipp and Shaji KumarConventional Cytogenetics in MyelomaLynda J. CampbellMulticolor Spectral Karyotyping in Multiple MyelomaJeffrey R. SawyerDetection of Chromosome 13 Deletions by Fluorescent In Situ HybridizationScott VanWier and Rafael FonsecaComparative Genomic Hybridization for Analysis of Changes in DNA Copy Number in Multiple MyelomaPulivarthi H. RaoSouthern Blotting of IgH Rearrangements in B-Cell DisordersEdna Yuen and Ross D. BrownIdentification and Assembly of V Genes As Idiotype-Specific DNA Fusion Vaccines in Multiple MyelomaSurinder S. Sahota, Mark Townsend, and Freda K. StevensonIdentification of Clonotypic IgH VDJ Sequences in Multiple MyelomaBrian J. Taylor, Jitra Kriangkum, Erin R. Strachan, Juanita Wizniak, and Linda M. PilarskiReal-Time Polymerase Chain Reaction of Immunoglobulin Rearrangements for Quantitative Evaluation of Minimal Residual Disease in MyelomaMara Compagno, Barbara Mantoan, Monica Astolfi, Mario Boccadoro, and Marco LadettoIncomplete DJH RearrangementsDavid González and Ramón Garcia-SanzIdentification of Malignant Plasma Cells by mRNA In Situ HybridizationRoss D. Brown and P. Joy HoThe SCID-hu Myeloma ModelJoshua Epstein and Shmuel YaccobyThe 5T2MM Murine Model of Multiple Myeloma: Maintenance and AnalysisKarin Vanderkerken, Kewal Asosingh, Angelo Willems, Hendrik De Raeve, Pedro Couck, Frans Gorus, Peter Croucher, and Ben Van CampDetermination of Telomerase Activity and Telomere LengthKai-da Wu and Malcolm A. S. MooreDendritic Cell/Myeloma Hybrid VaccineDajing Xia, Tim Chan, and Jim XiangGenetically Engineered Myeloma Cell VaccineSiguo Hao, Tim Chan, and Jim XiangAn In Vitro Osteoclast-Forming Assay to Measure Myeloma Cell-Derived Osteoclast-Activating FactorsAndrew C. W. Zannettino, Amanda N. Farrugia, L. Bik To, and Gerald J. AtkinsClonality Detection of the Expanded T-Cell Populations in Patients With Multiple MyelomaDaniel M-Y. SzeDetecting Mismatch Repair Defects in MyelomaMark Velangi, Elizabeth Matheson, Andrew Hall, and Julie IrvingMethylation-Specific Polymerase Chain ReactionOliver Galm and James G. HermanMaking Sense of DNA Microarray DataWei-min Liu, Sunhee K. Ro, and Walter H. KochIndex

Editorial Reviews

From the reviews:"...extremely informative...a great reference for researchers or students with an interest in the etiology and treatment of Multiple Myeloma." - The Journal of the Association of Genetic Technologists"Audience: Hematologists, oncologists, and other clinicians . as well as biomedical researchers in the field of hematology and immunology. . The book is divided into 23 chapters, including schematic diagrams, figures, color plates, and tables, as well as an index of important terms at the end. . covers clinical as well as experimental aspects of multiple myeloma and provides an important set of information for clinicians and researchers, in different fields of biomedicine, including hematology, oncology, molecular diagnostic, clinical immunology, osteoimmunology, molecular-biology, genetics, and bioinformatics." (Danka Grcevic, Croatian Medical Journal, Vol. 48, October, 2007)