Mycobacterium Tuberculosis Protocols by Tanya ParishMycobacterium Tuberculosis Protocols by Tanya Parish

Mycobacterium Tuberculosis Protocols

EditorTanya Parish, Neil G. Stoker

Paperback | November 9, 2010

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Leading investigators with extensive practical knowledge and experience describe their best methods for studying the tuberculosis pathogen. Packed with step-by-step instructions to ensure successful results, these methods range from basic handling techniques to the application of functional genomics. Highlights include methods for the basic safety and culture of M. tuberculosis, fractionation of the bacterium (nucleic acids, lipids, culture filtrate, and capsule), the analysis of gene expression (start site mapping, real-time PCR microarrays, and proteomics), the growth of the bacterium in macrophages and low oxygen, cytological analysis of the bacteria, and diagnostics. Highly practical and accessible, Mycobacterium tuberculosis Protocols utilizes advanced functional genomics and mutagenesis methodologies to provide both experimental and clinical investigators all the powerful techniques needed to illuminate the molecular biology of tuberculosis and its interactions with host cells, and so drive work on the wide variety of emerging therapeutic opportunities.
Title:Mycobacterium Tuberculosis ProtocolsFormat:PaperbackDimensions:418 pagesPublished:November 9, 2010Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:161737184X

ISBN - 13:9781617371844

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Table of Contents

Plasmid VectorsNicola Casali and Sabine EhrtIsolation of DNA from Mycobacterium tuberculosisPaul D. van Helden, Thomas C. Victor, Robin M. Warren, and Eileen G. van HeldenExtraction of RNA from Intracellular Mycobacterium tuberculosis: Methods, Considerations, and ApplicationsIrene M. Monahan, Joseph A. Mangan, and Philip D. ButcherTransposon Mutagenesis in Mycobacteria Using Conditionally Replicating MycobacteriophagesStoyan S. Bardarov, Svetoslav S. Bardarov, Jr., and William R. Jacobs, JrGene Replacement and Transposon Delivery Using the Negative Selection Marker sacBMary Jackson, Luis Reinaldo Camacho, Brigitte Gicquel, and Christophe GuilhotGene Replacement Using Pretreated DNABhavna G. Gordhan and Tanya ParishGene Replacement in Mycobacterium tuberculosis and Mycobacterium bovis BCG Using rpsL+ as a Dominant Negative Selectable MarkerPeter Sander, Burkhard Springer, and Erik C. BöttgerTranscription Start-Site MappingFarahnaz Movahedzadeh, Jorge A. Gonzalez-Y-Merchand, and Robert A. CoxFluorescence and Brightfield Cytology of Live M. tuberculosis CellsRory P. Cooney, Natalie J. Garton, and Michael R. BarerPhage Replication Technology for Diagnosis and Drug Susceptibility TestingRuth McNerneyDetection of Mutations in Mycobacterium tuberculosis by a Dot Blot Hybridization StrategyThomas C. Victor and Paul D. van HeldenRestriction Fragment Length Polymorphism Typing of MycobacteriaDick van Soolingen, Petra E. W. de Haas, and Kristin KremerPreparation of Culture Filtrate Proteins from Mycobacterium tuberculosisIda Rosenkrands and Peter AndersenAnalysis of the Capsule of Mycobacterium tuberculosisMamadou Daffé and Marie-Antoinette LanéelleAnalysis of the Lipids of Mycobacterium tuberculosisRichard A. Slayden and Clifton E. Barry, 3rdIn Vitro Model of Hypoxically Induced Nonreplicating Persistence of Mycobacterium tuberculosisLawrence G. WayneMacrophage Virulence AssaysPauline T. Lukey and Elizabeth U. HookerAnalysis of Mycobacterium-Infected Macrophages by Immunoelectron Microscopy and Cell FractionationWandy Beatty and David G. RussellReal-Time PCR Using Molecular Beacons: A New Tool to Identify Point Mutations and to Analyze Gene Expression in Mycobacterium tuberculosisRiccardo Manganelli, Sanjay Tyagi, and Issar SmithElectronic Access to Mycobacterium tuberculosis Sequence DataJulian ParkhillProteomicsJoanna C. Betts and Marjorie A. SmithFunctional Genomics of Mycobacterium tuberculosis Using DNA MicroarraysMichael Wilson, Martin Voskuil, Dirk Schnappinger, and Gary K. SchoolnikStorage of Mycobacterial StrainsKristin Kremer, Tridia van der Laan, and Dick van SoolingenSafety in the LaboratoryHeidi Alderton and Debbie Smith

Editorial Reviews

...a very useful book for the mycobacteria research community. It is a specialist's cookbook, perfect if you want to establish a new method that is outside your scope of expertise. Individual chapters are clearly divided into introduction, materials, methods and an extensive list of notes. -- International Journal of Medical Microbiology"For those engaged in mycobacterial research (and possibly other unrelated genera) which involves genetics, biochemistry and immunology, this textbook is essential and, moreover, I would recommend it as reading material for students undertaking molecular biology experimentation." -Today's Life Science