Nanoimaging: Methods and Protocols by Alioscka A. SousaNanoimaging: Methods and Protocols by Alioscka A. Sousa

Nanoimaging: Methods and Protocols

EditorAlioscka A. Sousa, Michael J. Kruhlak

Hardcover | October 21, 2012

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For more than a century, microscopy has been a centerpiece of extraordinary discoveries in biology.  Along the way, remarkable imaging tools have been developed allowing scientists to dissect the complexity of cellular processes at the nano length molecular scales.  Nanoimaging: Methods and Protocols presents a diverse collection of microscopy techniques and methodologies that provides guidance to successfully image cellular molecular complexes at nanometer spatial resolution.  The book's four parts cover: (1) light microscopy techniques with a special emphasis on methods that go beyond the classic diffraction-limited imaging; (2) electron microscopy techniques for high-resolution imaging of molecules, cells and tissues, in both two and three dimensions; (3) scanning probe microscopy techniques for imaging and probing macromolecular complexes and membrane surface topography;  and (4) complementary techniques on correlative microscopy, soft x-ray tomography and secondary ion mass spectrometry imaging.  Written in the successful format of the Methods in Molecular BiologyT series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Nanoimaging: Methods and Protocols highlights many of the most exciting possibilities in microscopy for the investigation of biological structures at the nano length molecular scales.
Title:Nanoimaging: Methods and ProtocolsFormat:HardcoverDimensions:524 pagesPublished:October 21, 2012Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1627031367

ISBN - 13:9781627031363

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Table of Contents

1. Introduction: Nanoimaging Techniques in BiologyAlioscka A. Sousa and Michael J. Kruhlak  Part I. Light Microscopy  2. Live-Cell Imaging of Vesicle Trafficking and Divalent Metal Ions by Total Internal Reflection Fluorescence (TIRF) Microscopy                Merewyn K. Loder, Takashi Tsuboi, and Guy A. Rutter 3. 4Pi MicroscopyRoman Schmidt, Johann Engelhardt, and Marion Lang 4. Fluorescence in situ Hybridization Applications for Super-Resolution 3D Structured Illumination MicroscopyYolanda Markaki, Daniel Smeets, Marion Cremer, and Lothar Schermelleh 5. Two-Color STED Imaging of Synapses in Living Brain SlicesJan Tønnesen, and U. Valentin Nägerl 6. Super-Resolution Imaging by Localization MicroscopyDylan M. Owen, Astrid Magenau, David Williamson, and Katharina Gaus 7. High-Content Super-Resolution Imaging of Live Cell by uPAINTGrégory Giannone, Eric Hosy, Jean-Baptiste Sibarita, Daniel Choquet, and Laurent Cognet  8. Super-Resolution Fluorescence Imaging with Blink MicroscopyChristian Steinhauer, Michelle S. Itano, and Philip Tinnefeld 9. Photoswitchable Fluorophores for Single-Molecule Localization MicroscopyKieran Finan, Benjamin Flottmann, and Mike Heilemann 10. Single-Molecule Tracking of mRNA in Living CellsMai Yamagishi, Yoshitaka Shirasaki, and Takashi Funatsu  Part II. Electron Microscopy 11. Semiautomatic, High-Throughput, High-Resolution Protocol for Three-Dimensional Reconstruction of Single Particles in Electron MicroscopyCarlos Oscar Sorzano, J. M. de la Rosa Trevín, J. Otón, J. J. Vega, J. Cuenca, A. Zaldívar-Peraza, J. Gómez-Blanco, J. Vargas, A. Quintana, R. Marabini, and J. M. Carazo 12. Mass Mapping of Amyloid Fibrils in the Electron Microscope using STEM ImagingAlioscka A. Sousa and Richard D. Leapman 13. Elemental Mapping by Electron Energy Loss Spectroscopy in BiologyMaria A. Aronova and Richard D. Leapman 14. Cellular Nanoimaging by Cryo Electron Tomography Roman I. Koning and Abraham J. Koster 15. Large-Volume Reconstruction of Brain Tissue from High-Resolution Serial Section Images Acquired by SEM-Based Scanning Transmission Electron MicroscopyMasaaki Kuwajima, John M. Mendenhall, and Kristen M. Harris 16. 3D Imaging of Cells and Tissues by Focused Ion Beam/Scanning Electron Microscopy (FIB/SEM)Damjana Drobne 17. Preparation of Gold Nanocluster Bioconjugates for Electron MicroscopyChristine L. Heinecke and Christopher J. Ackerson  Part III. Scanning Probe Microscopy 18. Atomic Force Microscopy Imaging of Macromolecular ComplexesS. Santos, D.J. Billingsley, and N.H. Thomson 19. Imaging of Transmembrane Proteins Directly Incorporated Within Supported Lipid Bilayers Using Atomic Force MicroscopyDaniel Levy and Pierre-Emmanuel Milhiet 20. Functional AFM Imaging of Cellular Membranes Using Functionalized TipsLilia A. Chtcheglova and Peter Hinterdorfer 21. Near-Field Scanning Optical Microscopy for High Resolution Membrane Studies          Heath A. Huckabay, Kevin P. Armendariz, William H. Newhart, Sarah M. Wildgen, and Robert C. Dunn  Part IV. Complementary Techniques 22. Correlative Fluorescence and EFTEM Imaging of the Organized Components of the Mammalian NucleusMichael J. Kruhlak 23. High Data Output Method for 3-D Correlative Light-Electron Microscopy Using Ultrathin Cryosections Katia Cortese, Giuseppe Vicidomini, Maria C. Gagliani, Patrizia Boccacci, Alberto Diaspro, and Carlo Tacchetti 24. Correlative Optical and Scanning Probe Microscopies for Mapping Interactions at MembranesChristopher M. Yip 25. Nanoimaging Cells Using Soft X-Ray TomographyDilworth Y. Parkinson, Lindsay R. Epperly, Gerry McDermott, Mark A. Le Gros, Rosanne M. Boudreau, and Carolyn A. Larabell 26. Secondary Ion Mass Spectrometry Imaging of Biological Membranes at High Spatial ResolutionHaley A. Klitzing, Peter K. Weber, and Mary L. Kraft