Neurodegeneration: Methods and Protocols by Giovanni ManfrediNeurodegeneration: Methods and Protocols by Giovanni Manfredi

Neurodegeneration: Methods and Protocols

EditorGiovanni Manfredi, Hibiki Kawamata

Hardcover | September 15, 2011

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Neurodegenerative disorders, such as Alzheimer's and Parkinson's disease are a growing burden on our society. Understanding and treating these common diseases is one of the major current challenges for the biomedical field. In Neurodegeneration: Methods and Protocols, experts in the field tackle this challenge and present cutting-edge analytical and technological approaches to study the mechanisms underlying neurodegenerative processes. Organizes into five sections, chapters detail protocols for isolating and culturing cells from the nervous system, production and use of animal models, characterization of cell death, analytical tools to study disease mechanisms, and therapeutic approaches for neurodegeneration. Written in the successful Methods in Molecular BiologyT  series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.Authoritative and easily accessible, Neurodegeneration: Methods and Protocols serves as an ideal guide to both novice and professional researchers in the field of neurodegeneration.
Title:Neurodegeneration: Methods and ProtocolsFormat:HardcoverDimensions:527 pagesPublished:September 15, 2011Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617793272

ISBN - 13:9781617793271

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Table of Contents

Part I. Neurodegeneration 1. Introduction to Neurodegenerative Diseases and Related Techniques             Hibiki Kawamata and Giovanni Manfredi  2. In vivo and In vitro Determination of Cell Death Markers in Neurons            Nicole Naniche, Daniela Sua, and Piera Pasinelli 3. Degenerating Processes Identified by Electron Microscopic Immunocytochemical Methods            Teresa A. Milner, Elizabeth M. Waters, Danielle C. Robinson, and Joseph P. Pierce   Part II. Neural Cell Culture Techniques 4. Isolation and Culture of Neurons and Astrocytes from the Mouse Brain Cortex            Hyun-Jeong Kim and Jordi Magrané 5. Isolation and Culture of Postnatal Spinal Motoneurons            Carol Milligan and David Gifondorwa 6. Converting Human Pluripotent Stem Cells to Neural Tissue and Neurons to Model Neurodegeneration            Stuart M. Chambers, Yvonne Mica, Lorenz Studer, and Mark J. Tomishima 7. Neural Differentiation of Induced Pluripotent Stem Cells            Mark Denham and Mirella Dottori  Part III. Models of Neurodegeneration 8. Developing Yeast Models of Human Neurodegenerative Disorders            Alejandro Ocampo and Antoni Barrientos 9. Modeling Dopamine Neuron Degeneration in C. elegans            Michelle L. Tucci, Adam J. Harrington, Guy A. Caldwell, and Kim A. Caldwell 10. Role of the Proteasome in Fly Models of Neurodegeneration                 Chun-Hung Yeh, Marlon Jansen, and Thomas Schmidt-Glenewinkel 11. Modeling Neurodegenerative Diseases in Zebrafish Embryos            Angela Laird and Wim Robberecht 12. Practical Considerations of Genetic Rodent Models for Neurodegenerative Diseases             Kindiya Geghman and Chenjian Li 13. Modeling Focal Cerebral Ischemia In vivo            Katherine Jackman, Alexander Kunz, and Costantino Iadecola 14. Pharmacological Models of Parkison's Disease in Rodents            Peter Klivenyi and Laszlo Vecsei 15. Behavioral Phenotyping of Mouse Models of Neurodegeneration            Magali Dumont  Part IV. Neurodegenerative Disease Mechanisms - Proteinopathies 16. Characterization of Amyloid Deposits in Neurodegenerative Diseases            Ruben Vidal and Bernardino Ghetti 17. Detection of Protein Aggregates in Neurodegenerative Diseases            Han-xiang Deng, Eileen H. Bigio, and Teepu Siddique  18. Assessment of Proteasome Impairment and Accumulation/Aggregation of Ubiquitinated Proteins in Neuronal Cultures            Natura Myeku, Maria Jose Metcalfe, Qian Huang, and Maria Figueiredo-Pereira   Part V. Neurodegenerative Disease Mechanisms - Mitochondria 19. Measurements of Threshold of Mitochondrial Transition Pore Opening in Intact and Permeabilized Cells by Flash Photolysis of Caged Calcium            Andrey Y. Abramov and Michael R. Duchen 20. Isolation and Functional Assessment of Mitochondria from Small Amounts of Mouse Brain Tissue            Christos Chinopoulos, Steven F. Zhang, Bobby Thomas, Vadim Ten, and Anatoly A. Starkov  21. Monitoring Mitophagy in Neuronal Cell Cultures               Jianhui Zhu, Ruben K. Dagda, and Charleen T. Chu  Part VI. Neurodegenerative Disease Mechanisms - Biological functions 22. Analysis of Vesicular Trafficking in Primary Neurons by Live Imaging            Davide Tampellini and Gunnar K. Gouras  23. Surface Trafficking of Sodium Channels in Cells and in Hippocampal Slices            Doo Yeon Kim and Dora M. Kovacs 24. Imaging Presynaptic Exocytosis in Corticostriatal Slices            Minerva Yue, David Sulzer, and Nigel Bamford 25. Microscopic Imaging of Intracellular Calcium in Live Cells using Lifetime based Ratiometric Measurements of Oregon Green BAPTA-1            Carli Lattarulo, Diana Thyssen, Kishore V. Kuchibholta, Bradley T. Hyman, and Brian J. Bacskai 26. Electrophysiological Characterization of Neuromuscular Synaptic Dysfunction in Mice                   Yoshie Sugiura and Weichun Lin 27. Determination of Neurotransmitter Levels in Models of Parkinson's Disease by HPLC-ECD            Lichuan Yang and Flint M. Beal 28. Designing, Performing, and Interpreting a Microarray-based Gene Expression Study             Giovanni Coppola   Part VII. Therapeutic Approaches in Neurodegeneration 29. Adeno-associated Viral Gene Transfer for Neurodegenerative Diseases            Peter F. Morgenstern, Roberta Marongiu, Sergei A. Musatov, and Michael G. Kaplitt 30. Specific Gene Silencing using RNAi in Cell Culture                     Chunxing Yang, Linghua Qiu, and Zuoshang Xu 31. Stem Cell Transplantation for Spinal Cord Neurodegeneration            Angelo C. Lepore and Nicholas J. Maragakis 32. Evaluation of Histone Deacetylase Inhibitors as Therapeutics for Neurodegenerative Diseases            Elisabetta Soragni, Chunping Xu, Andrew Cooper, Heather L. Plasterer, James R. Rusche, and Joel M. Gottesfeld