Neurodegeneration: Methods and Protocols by Giovanni ManfrediNeurodegeneration: Methods and Protocols by Giovanni Manfredi

Neurodegeneration: Methods and Protocols

byGiovanni ManfrediEditorHibiki Kawamata

Hardcover | September 15, 2011

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Neurodegenerative disorders, such as Alzheimer's and Parkinson's disease are a growing burden on our society. Understanding and treating these common diseases is one of the major current challenges for the biomedical field. In Neurodegeneration: Methods and Protocols, experts in the field tackle this challenge and present cutting-edge analytical and technological approaches to study the mechanisms underlying neurodegenerative processes. Organizes into five sections, chapters detail protocols for isolating and culturing cells from the nervous system, production and use of animal models, characterization of cell death, analytical tools to study disease mechanisms, and therapeutic approaches for neurodegeneration. Written in the successful Methods in Molecular BiologyT  series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls.

Authoritative and easily accessible, Neurodegeneration: Methods and Protocols serves as an ideal guide to both novice and professional researchers in the field of neurodegeneration.

Title:Neurodegeneration: Methods and ProtocolsFormat:HardcoverDimensions:512 pagesPublished:September 15, 2011Publisher:Springer-Verlag/Sci-Tech/TradeLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617793272

ISBN - 13:9781617793271

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Table of Contents

Part I. Neurodegeneration


1. Introduction to Neurodegenerative Diseases and Related Techniques

            Hibiki Kawamata and Giovanni Manfredi


2. In vivo and In vitro Determination of Cell Death Markers in Neurons

            Nicole Naniche, Daniela Sua, and Piera Pasinelli


3. Degenerating Processes Identified by Electron Microscopic Immunocytochemical Methods

            Teresa A. Milner, Elizabeth M. Waters, Danielle C. Robinson, and Joseph P. Pierce



Part II. Neural Cell Culture Techniques


4. Isolation and Culture of Neurons and Astrocytes from the Mouse Brain Cortex

            Hyun-Jeong Kim and Jordi Magrané


5. Isolation and Culture of Postnatal Spinal Motoneurons

            Carol Milligan and David Gifondorwa


6. Converting Human Pluripotent Stem Cells to Neural Tissue and Neurons to Model Neurodegeneration

            Stuart M. Chambers, Yvonne Mica, Lorenz Studer, and Mark J. Tomishima


7. Neural Differentiation of Induced Pluripotent Stem Cells

            Mark Denham and Mirella Dottori



Part III. Models of Neurodegeneration


8. Developing Yeast Models of Human Neurodegenerative Disorders

            Alejandro Ocampo and Antoni Barrientos


9. Modeling Dopamine Neuron Degeneration in C. elegans

            Michelle L. Tucci, Adam J. Harrington, Guy A. Caldwell, and Kim A. Caldwell


10. Role of the Proteasome in Fly Models of Neurodegeneration    

            Chun-Hung Yeh, Marlon Jansen, and Thomas Schmidt-Glenewinkel


11. Modeling Neurodegenerative Diseases in Zebrafish Embryos

            Angela Laird and Wim Robberecht


12. Practical Considerations of Genetic Rodent Models for Neurodegenerative Diseases

            Kindiya Geghman and Chenjian Li


13. Modeling Focal Cerebral Ischemia In vivo

            Katherine Jackman, Alexander Kunz, and Costantino Iadecola


14. Pharmacological Models of Parkison's Disease in Rodents

            Peter Klivenyi and Laszlo Vecsei


15. Behavioral Phenotyping of Mouse Models of Neurodegeneration

            Magali Dumont



Part IV. Neurodegenerative Disease Mechanisms - Proteinopathies


16. Characterization of Amyloid Deposits in Neurodegenerative Diseases

            Ruben Vidal and Bernardino Ghetti


17. Detection of Protein Aggregates in Neurodegenerative Diseases

            Han-xiang Deng, Eileen H. Bigio, and Teepu Siddique


18. Assessment of Proteasome Impairment and Accumulation/Aggregation of Ubiquitinated Proteins in Neuronal Cultures

            Natura Myeku, Maria Jose Metcalfe, Qian Huang, and Maria Figueiredo-Pereira



Part V. Neurodegenerative Disease Mechanisms - Mitochondria


19. Measurements of Threshold of Mitochondrial Transition Pore Opening in Intact and Permeabilized Cells by Flash Photolysis of Caged Calcium

            Andrey Y. Abramov and Michael R. Duchen


20. Isolation and Functional Assessment of Mitochondria from Small Amounts of Mouse Brain Tissue

            Christos Chinopoulos, Steven F. Zhang, Bobby Thomas, Vadim Ten, and Anatoly A. Starkov


21. Monitoring Mitophagy in Neuronal Cell Cultures  

            Jianhui Zhu, Ruben K. Dagda, and Charleen T. Chu



Part VI. Neurodegenerative Disease Mechanisms - Biological functions


22. Analysis of Vesicular Trafficking in Primary Neurons by Live Imaging

            Davide Tampellini and Gunnar K. Gouras


23. Surface Trafficking of Sodium Channels in Cells and in Hippocampal Slices

            Doo Yeon Kim and Dora M. Kovacs


24. Imaging Presynaptic Exocytosis in Corticostriatal Slices

            Minerva Yue, David Sulzer, and Nigel Bamford


25. Microscopic Imaging of Intracellular Calcium in Live Cells using Lifetime based Ratiometric Measurements of Oregon Green BAPTA-1

            Carli Lattarulo, Diana Thyssen, Kishore V. Kuchibholta, Bradley T. Hyman, and Brian J. Bacskai


26. Electrophysiological Characterization of Neuromuscular Synaptic Dysfunction in Mice      

            Yoshie Sugiura and Weichun Lin


27. Determination of Neurotransmitter Levels in Models of Parkinson's Disease by HPLC-ECD

            Lichuan Yang and Flint M. Beal


28. Designing, Performing, and Interpreting a Microarray-based Gene Expression Study

            Giovanni Coppola



Part VII. Therapeutic Approaches in Neurodegeneration


29. Adeno-associated Viral Gene Transfer for Neurodegenerative Diseases

            Peter F. Morgenstern, Roberta Marongiu, Sergei A. Musatov, and Michael G. Kaplitt


30. Specific Gene Silencing using RNAi in Cell Culture        

            Chunxing Yang, Linghua Qiu, and Zuoshang Xu


31. Stem Cell Transplantation for Spinal Cord Neurodegeneration

            Angelo C. Lepore and Nicholas J. Maragakis


32. Evaluation of Histone Deacetylase Inhibitors as Therapeutics for Neurodegenerative Diseases

            Elisabetta Soragni, Chunping Xu, Andrew Cooper, Heather L. Plasterer, James R. Rusche, and Joel M. Gottesfeld

Editorial Reviews

From the reviews:"The authors describe various cell and molecular systems related to neurodegeneration research. . there are a lot of protocols and methods described by the book that can be used by medical students, graduate students, junior, and senior investigators. I heartily recommend this book to university and research institute communities working on neurodegeneration and regeneration." (Joseph J. Grenier,, September, 2013)"This is an authoritative collection of manuscripts tailored to bench scientists involved in neurodegenerative research. . It is intended for cell biologists, molecular biologists, biochemists, pathologists, and researchers in the field of neurodegeneration. . This book is most appropriate for the audience of basic scientists interested in the mechanics of experimental neurodegenerative work. For this audience, the book will find a suitable space on their lab benches." (Joseph I. Sirven, Doody's Book Reviews, April, 2012)