Neuronal Cell Culture: Methods and Protocols by Shohreh AminiNeuronal Cell Culture: Methods and Protocols by Shohreh Amini

Neuronal Cell Culture: Methods and Protocols

EditorShohreh Amini, Martyn K. White

Hardcover | August 24, 2013

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In Neuronal Cell Culture: Methods and Protocols, the latest aspects of the culture of neural cells are explored by experts in the field who also explain the practical and theoretical considerations of the techniques involved.  Starting with a general overview of the neuronal culturing principles that are described, this detailed volume covers cell line models for neural cells, the isolation and propagation of primary cultures, stem cells, transfection and transduction of neural cultures, and other more advanced techniques.  Written for the Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and easy to use, Neuronal Cell Culture: Methods and Protocols will be of interest to scientists at all levels developing cell culture models for neuroscientific studies.
Title:Neuronal Cell Culture: Methods and ProtocolsFormat:HardcoverDimensions:175 pages, 10 × 7.01 × 0 inPublished:August 24, 2013Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1627036393

ISBN - 13:9781627036399

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Table of Contents

1. General Overview of Neuronal Cell Culture            Jennifer Gordon, Shohreh Amini, and Martyn K. White 2. Considerations for the Use of SH-SY5Y Neuroblastoma Cells in Neurobiology            Jane Kovalevich and Dianne Langford 3. Cultured Cell Line Models of Neuronal Differentiation: NT2, PC12            Nune Darbinian 4. Murine Teratocarcinoma-Derived Neuronal Cultures            Prasun K. Datta 5. Isolation and Propagation of Primary Human and Rodent Embryonic Neural Progenitor Cells and Cortical Neurons            Armine Darbinyan, Rafal Kaminski, Martyn K. White, Nune Darbinian, and Kamel Khalili 6. Mouse Enteric Neuronal Cell Culture            Yonggang Zhang and Wenhui Hu 7. Preparation of Neural Stem Cells and Progenitors: Neuronal Production and Grafting Applications            Joseph F. Bonner, Christopher J. Haas, and Itzhak Fischer 8. Derivation of Neuronal Cells from Fetal Normal Human Astrocyte (NHA)            S. Ausim Azizi and Barbara Krynska 9. Slice Culture Modeling of Central Nervous System (CNS) Viral Infection            Kalen R. Dionne and Kenneth L. Tyler 10. Neurospheres and Glial Cell Cultures: Immunocytochemistry for Cell Phenotyping            Amanda Parker Struckhoff and Luis Del Valle 11. Transfection of Neuronal Cultures            Ilker Kudret Sariyer 12. Lentiviral Transduction of Neuronal Cells            Hassen S. Wollebo, Baheru Woldemichaele, and Martyn K. White 13. Compartmentalized Neuronal Cultures            Armine Darbinyan, Paul Pozniak, Nune Darbinian, Martyn K. White, and Kamel Khalili 14. Quantitative Assessment of Neurite Outgrowth Over Growth Promoting or Inhibitory Substrates            George M. Smith, Yingpeng Liu, and Jee W. Hong