Neuroproteomics by Ka Wan LiNeuroproteomics by Ka Wan Li


byKa Wan Li

Hardcover | May 25, 2011

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In the past few years, the application of proteomics to examine the molecular mechanisms underlying (mal-)functioning of the nervous system and brain disorders has risen steeply, which in many cases has yielded novel insights. As neuroproteomics is a new research field involving the use of a number of high-end analytical instruments and technologies, both promises and pitfalls may not be well appreciated by the researchers, and the design of a proper proteomics experiment may not be obvious.  Neuroproteomics brings together a collection of detailed protocols written by experienced professionals in the field in order to cover aspects of various dimensions of this new technology, together with some established methods that have supporting roles in the workflow of neuroproteomics. Written for the popular Neuromethods series, the chapters in this book contain step-by-step, easy to follow instruction along with practical tips and key implementation advice essential for successful implementation of the techniques. Authoritative and cutting-edge, Neuroproteomics will be of great assistance to all researchers seeking to design and execute their own proteomics experiments in an optimal way and continue moving forward this exciting area of study.
Title:NeuroproteomicsFormat:HardcoverDimensions:317 pagesPublished:May 25, 2011Publisher:Springer-Verlag/Sci-Tech/TradeLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617791105

ISBN - 13:9781617791109

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Table of Contents

Part I: Introduction 1. Neuroproteomics: Deciphering Brain Function and Disorders            Ka Wan Li Part II: Fractionation of Brain Regions, Organelles, and Protein Complexes 2. Dissection of Rodent Brain Regions            Sabine Spijker 3. Subcellular Fractionation of Brain Tissue Using Free Flow Electrophoresis            Markus Islinger, Joachim Kirsch, Sabine Angermüller, Ramona Rotaru, Afsaneh Abdolzade-Bavil, and Gerhard Weber 4. Isolation of Synapse Sub-Domains by Subcellular Fractionation Using Sucrose Density Gradient Centrifugation            Tatsuo Suzuki 5. Enrichment of Plasma Membranes from Small Brain Samples by Aqueous Polymer Two-Phase Systems            Jens Schindler 6. Identification and Characterization of Protein Complexes from Total Brain and Synaptoneurosomes: Heterogeneity of Molecular Complexes in Distinct Subcellular Domains            Silvia De Rubeis and Claudia Bagni 7. Analysis of Protein Complexes by 2D Blue Native/SDS-PAGE and Antibody-Shift Assay            Dong Yang, Xinyu Deng, Ying Jiang, and Fuchu He Part III: Analytical Tools for Neuroproteomics 8. 2-Dimensional Gel Electrophoresis-Based Proteomic Analysis of Brain Synapses            Karl-Heinz Smalla and Ursula Wyneken 9. 2-Dimensional BAC-SDS Polyacrylamide Gel Electrophoresis for the Fractionation and Identification of Synaptic Vesicle Proteins and the Presynaptic Active Zone            Joern Barth and Walter Volknandt 10. iTRAQ-Based LC-LC MALDI TOF/TOF MS Quantitative Analysis of Membrane Proteins from Human Glioma            Uros Rajcevic 11. OFFGEL-Isoelectric Focusing Fractionation for the Analysis of Complex Proteome            Emilie Ernoult and Catherine Guette 12. A 1D-PAGE/LC-ESI Linear Ion Trap Orbitrap MS Approach for the Analysis of Synapse Proteomes and Synaptic Protein Complexes            Ning Chen, Roel C. vd Schors and August B. Smit 13. SDS-PAGE Immuno-Blot Analysis            Patricia Klemmer 14. Phosphoproteomics by Highly Selective IMAC Protocol            Chia-Feng Tsai, Yi-Ting Wang, Pei-Yi Lin, and Yu-Ju Chen 15. Global Analysis of Ubiquitination            David Meierhofer and Peter Kaiser 16. High-Throughput High-Content Functional Image Analysis of Neuronal Proteins Implicated in Parkinson's Disease            Eva Blaas and Ronald E. van Kesteren Part IV: Analysis of Specific Brain Tissues and Fluid 17. Neuropeptidomics of the Mammalian Brain            Fang Xie, Elena V. Romanova, and Jonathan V. Sweedler 18. In-Depth Analysis of the Cerebrospinal Fluid Proteome and Biomarker Discovery: Abundant Protein Depletion Sample Pretreatment Method            Silvina A. Fratantoni and Connie R. Jimenez 19. Lipidomics of the Nervous System: Phospholipidomics by Liquid Chromatography Coupled to Mass Spectrometry or Tandem Mass Spectrometry            Su Chen Part V: Bioinformatics 20. Bioinformatics Procedures for Analysis of Quantitative Proteomics Experiments Using iTRAQ            Pim van Nierop and Maarten Loos 21. Statistical Analysis of Spectral Count Data Generated by Label-Free Tandem Mass Spectrometry-Based Proteomics            Thang V. Pham and Connie R. Jimenez