Neurotrophic Factors: Methods and Protocols by Stephen D SkaperNeurotrophic Factors: Methods and Protocols by Stephen D Skaper

Neurotrophic Factors: Methods and Protocols

EditorStephen D Skaper

Hardcover | February 25, 2012

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Nervous system development evolves from the well-orchestrated processes of neural induction, cell proliferation, differentiation, cell migration, survival, and synapse formation. Among these environmental cues, neurotrophic factors are secreted proteins that promote neurite outgrowth, neuronal cell differentiation and survival both in vivo and in vitro. Nerve growth factor (NGF) is the founding and best characterized member of the neurotrophin family of neurotrophic polypeptides. Neurotrophic Factors: Methods and Protocols presents a selection of protocols and procedures which make use of cellular, tissue, and whole animal models which can be applied to the investigation of neurotrophic factors and other agents impacting on these systems. Chapters cover a wide-range of topics such as dealing with the culture of neurons and glia from the central and peripheral nervous systems, neuron-glia co-culture models, and cell-based assays for the evaluation of neuroprotective molecules, as well as assays which can be applied to the study of agents with neuroregenerative potential. Protocols describing viral- and nanoparticle-based delivery methods to neural cells are also presented, following by chapters dealing with organotypic slice culture protocols. Lastly, several chapters are dedicated to in vivo lesion models of relevance to nervous system pathology, which can be applied to the investigation of neurotrophic factors and peptides. Written in the successful Methods in Molecular BiologyT series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Neurotrophic Factors: Methods and Protocols seeks to serve both professionals and novices alike with its well-honed methodologies in an effort to further our knowledge of what has been described as the last frontier of science.
Title:Neurotrophic Factors: Methods and ProtocolsFormat:HardcoverDimensions:435 pagesPublished:February 25, 2012Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617795356

ISBN - 13:9781617795350

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Table of Contents

1. The Neurotrophin Family of Neurotrophic Factors - An Overview            Stephen D. Skaper 2. Neuronal Growth-Promoting and Inhibiting Cues in Neuroprotection and Neurodegeneration            Stephen D. Skaper 3. Culture of Rat Cerebellar Granule Neurons and Application to Identify Neuroprotective Agents            Laura Facci and Stephen D. Skaper 4. Isolation and Culture of Neural Progenitor Cells from Rat Postnatal Cerebellum            Morena Zusso and Patrizia Debetto 5. Culture of Rodent Cortical and Hippocampal Neurons            Laura Facci and Stephen D. Skaper 6. Amyloid ?-Peptide Neurotoxicity Assay Using Cultured Rat Cortical Neurons            Laura Facci and Stephen D. Skaper 7. Culture of Neonatal Rodent Microglia, Astrocytes, and Oligodendrocytes from Cortex and Spinal Cord            Stephen D. Skaper, Carla Argentini, and Massimo Barbierato 8. Central Nervous System Neuron-Glia Co-Culture Models            Stephen D. Skaper and Laura Facci 9. Culture and Characterization of Rat Mesencephalic Dopaminergic Neurons            Stephen D. Skaper, Giulia Mercanti, and Laura Facci 10. Preparation of Adult Spinal Cord Motor Neuron Cultures Under Serum-Free Conditions            Jose V. Montoya-Gacharna, Jhon Jairo Sutachan, Wai Si Chan, Alexandra Sideris, Thomas J.J. Blanck, and Esperanza Recio-Pinto 11. Rodent Retinal Ganglion Cell Cultures            Stephen D. Skaper 12. Culture of Purified Glial Cell Populations from Optic Nerve            Stephen D. Skaper 13. Isolation and Culture of Rat Cone Photoreceptor Cells            Stephen D. Skaper 14. Rat Retina Pigmented Epithelial Cells            Stephen D. Skaper 15. Mammalian Growth Cone Turning Assays Identify Distinct Cell Signalling Mechanisms that Underlie Axon Growth, Guidance and Regeneration            Andrew J. Murray, Andrew G. Peace, Steven J. Tucker  and Derryck A. Shewan 16. Culture of Dissociated Sensory Neurons from Dorsal Root Ganglia of Postnatal and Adult Rats            Davina E. Owen and Julie Egerton 17. Culture and Proliferation of Highly Purified Adult Schwann Cells from Rat, Dog, and Man            Kirsten Haastert-Talini 18. Use of PC12 Cells and Rat Superior Cervical Ganglion Sympathetic Neurons as Models for Neuroprotective Assays Relevant to Parkinson's Disease            Cristina Malagelada Grau and Lloyd A. Greene 19. Compartmented Chambers for Studying Neurotrophic Factor Action            Stephen D. Skaper 20. Preparation and Culture of Adrenal Chromaffin Cells            Natalia Domínguez, Miriam Rodríguez, J. David Machado, and Ricardo Borges 21. Indirect Immunofluorescence Staining of Cultured Neural Cells            Massimo Barbierato, Carla Argentini, and Stephen D. Skaper 22. Neurite Outgrowth Assessment Using High Content Analysis Methodology            Nicholas M. Radio 23. Dissociated Cell Culture for Testing Effects of Carbon Nanotubes on Neuronal Growth            William Lee and Vladimir Parpura 24. High-Resolution Imaging and Evaluation of Spines in Organotypic Hippocampal Slice Cultures            Frederik Sündermann, Nataliya Golovyashkina, Roland Brandt, Lidia Bakota, and Christian Tackenberg 25. Imaging Amyloid Precursor Protein In Vivo - An Axonal Transport Assay            Tomás L. Falzone and Gorazd B. Stokin 26. The Use of Specific AAV Serotypes to Stably Transduce  Primary CNS Neuron Cultures            Saafan Z. Malik, Margie Maronski, Marc A. Dichter and Deborah J. Watson 27. Preparation and Characterization of Biocompatible Chitosan Nanoparticles for Targeted Brain Delivery of Peptides            Secil Caban, Yilmaz Capan, Patrick Couvreur, and Turgay Dalkara 28.  [3H]Serotonin Release Assay Using Antigen-Stimulated Rat Peritoneal Mast Cells            Stephen D. Skaper and Laura Facci 29. Rat Hippocampal Slice Culture Models for the Evaluation of Neuroprotective Agents            Elisabetta Gerace, Elisa Landucci, Tania Scartabelli, Flavio Moroni, and Domenico E. Pellegrini-Giampietro 30. A 6-Hydroxydopamine In Vivo Model of Parkinson's Disease            Giulia Mercanti, Gianfranco Bazzu, and Pietro Giusti 31. Brain Microdialysis in Freely Moving Animals            Gianfranco Bazzu, Alice Biosa, Donatella Farina, Ylenia Spissu, Giammario Calia, Sonia Dedola, Gaia Rocchitta, Rossana Migheli, Pier Andrea Serra, and Maria Speranza Desole 32. Evaluating Motor Neuron Death in Neonatal Rats Subjected to Sciatic Nerve Lesion            Andre Schwambach Vieira, Alexandre Cesar Santos de Rezende, and Fabio Rogerio 33. Rodent Spinal Cord Injury Model and Application of Neurotrophic Factors for Neuroprotection            Hari Shanker Sharma and Aruna Sharma