Neutrophil Methods and Protocols by Mark T. QuinnNeutrophil Methods and Protocols by Mark T. Quinn

Neutrophil Methods and Protocols

EditorMark T. Quinn

Paperback | December 10, 2010

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This book provides a concise set of protocols for assessing basic neutrophil functions, investigating specialized areas in neutrophil research, and completing step-by-step diagnostic assays of common neutrophil disorders. Each of the protocols is written by leading researchers in the field and includes hints for success, as well as guidance for troubleshooting. Scientists and clinicians will find this collection an invaluable aid.
Title:Neutrophil Methods and ProtocolsFormat:PaperbackDimensions:557 pagesPublished:December 10, 2010Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617377791

ISBN - 13:9781617377792

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Table of Contents

NeutrophilsMethods and ProtocolsEdited byMark T. QuinnFrank R. DeLeoGary M. Bokoch ContentsDedication iPreface iiContributors vPART I: NEUTROPHILS: AN OVERVIEW1. The Role of Neutrophils in the Immune System: An OverviewHarry L. MalechPART II: NEUTROPHIL ISOLATION AND SUBCELLULAR FRACTIONATION2. Isolation of Human Neutrophils From Venous BloodWilliam M. Nauseef 3. Neutrophil Isolation From Non-Human SpeciesDaniel W. Siemsen, Igor A. Schepetkin, Lilia N. Kirpotina, Benfang Lei, and Mark T. Quinn4. Subcellular Fractionation of Human Neutrophils and Analysis of Subcellular MarkersLene Udby and Niels Borregaard PART III. BIOCHEMISTRY, BIOLOGY AND SIGNAL TRANSDUCTION OF NEUTROPHILS5. Rac and Rap GTPase Activation AssaysUlla G. Knaus, Alison Bamberg, and Gary M. Bokoch6. Measurement of Phospholipid Metabolism in Intact NeutrophilsSusan Sergeant and Linda C. McPhail7. Analysis of Protein Phosphorylation in Human NeutrophilsJamel El-Benna and Pham My-Chan Dang8. Mitogen-Activated Protein Kinase AssaysMartine Torres9. Transduction of Proteins into Intact NeutrophilsTieming Zhao and Gary M. Bokoch10. Optical Methods for the Measurement and Manipulation of Cytosolic Free Calcium in NeutrophilsEsther J. Hillson, Sharon Dewitt, and Maurice B. Hallett11. Analysis of Electrophysiological Properties and Responses of NeutrophilsDeri Morgan and Thomas E. DeCoursey12. Analysis of Neutrophil ApoptosisEmma L. Taylor, Adriano G. Rossi, Ian Dransfield, and Simon P. HartPART IV. NEUTROPHIL ADHESION AND CHEMOTAXIS13. Optical and Fluorescence Detection of Neutrophil Integrin ActivationUlrich Y. Schaff, Melissa R. Sarantos, Harold Ting, and Scott I. Simon14. Analysis of Neutrophil Polarization and ChemotaxisMary A. Lokuta, Paul A. Nuzzi, and Anna Huttenlocher15. Quantifying and Localizing Actin Free Barbed Ends in NeutrophilsMichael Glogauer16. Measurement of Neutrophil Adhesion Under Conditions Mimicking Blood FlowMark A. Jutila, Bruce Walcheck, Robert Bargatze, and Aiyappa Palecanda17. Model Systems to Investigate Neutrophil Adhesion and ChemotaxisNancy A. Louis, Eric Campbell, and Sean P. Colgan PART V. NEUTROPHIL PHAGOCYTOSIS AND BACTERICIDAL ACTIVITY18. Immunofluorescence and Confocal Microscopy of NeutrophilsLee-Ann H. Allen19. Assessment of Phagosome Formation and Maturation by Fluorescence MicroscopyBenjamin E. Steinberg and Sergio Grinstein20. Analysis of Neutrophil Membrane Traffic During PhagocytosisPer Lönnbro, Pontus Nordenfelt, and Hans Tapper21. Analysis of Neutrophil Bactericidal ActivityJessie N. Green, Christine C. Winterbourn, and Mark B. Hampton22. A Skin Chamber Technique as a Human Model for Studies of Aseptic Inflammatory ReactionsPer Follin and Claes DahlgrenPART VI. NADPH OXIDASE AND PRODUCTION OF REACTIVE OXYGEN SPECIES23. Measurement of Respiratory Burst Products Generated by Professional PhagocytesClaes Dahlgren, Anna Karlsson, and Johan Bylund24. The K-562 Cell Model for Analysis of Neutrophil NADPH Oxidase FunctionThomas L. Leto, Mark C. Lavigne, Neda Homoyounpour, Kristen Lekstrom, Gilda Linton, Harry L. Malech, and Isabelle de Mendez25. Cell-free Assays: The Reductionist Approach to the Study of NADPH Oxidase Assembly Shahar Molshanski-Mor, Ariel Mizrahi, Yelena Ugolev, Iris Dahan, Yevgeny Berdichevsky, and Edgar Pick26. Immunoaffinity Purification of Human Phagocyte Flavocytochrome b and Analysis of Conformational DynamicsRoss M. Taylor and Algirdas J. JesaitisPART VII. ANALYSIS OF NEUTROPHIL GENE EXPRESSION AND TRANSCRIPTION FACTORS27. Genome-scale Transcript Analyses with Human NeutrophilsScott D. Kobayashi, Dan E. Sturdevant, and Frank R. DeLeo28. Fast and Accurate Quantitative Analysis of Cytokine Gene Expression in Human Neutrophils by Reverse Transcription Real-Time PCRNicola Tamassia, Marco A. Cassatella, and Flavia Bazzoni 29. Detection of Intact Transcription Factors in Human NeutrophilsPatrick P. McDonald and Richard D. Ye PART VIII. NEUTROPHIL DEFECTS AND DIAGNOSIS30. Disorders of Neutrophil Function: An OverviewMary C. Dinauer 31. Diagnostic Assays for Chronic Granulomatous Disease and Other Neutrophil DisordersHouda Zghal Elloumi and Steven M. Holland32. Diagnostic Assays for Myeloperoxidase DeficiencyWilliam M. Nauseef

Editorial Reviews

From the reviews:"...a carefully compiled collection of protocols that allow for study of many aspects of the neutrophil." -Doody's Book Review, Weighted Numerical Score:85-3 Stars"This excellent book is one of the latest in the 'Methods in Molecular Biology' series . . it comes beautifully presented and bound in a hardback cover and would grace any bookshelf. I would urge all self-respecting laboratories working with neutrophils - whether in research or diagnostics - to purchase one immediately. . I was impressed by this book's comprehensive provision of protocols, addressing all aspects of neutrophil function and physiology, and including an excellent section on diagnosis of both inherited and non-inherited neutrophil disorders." (Lynn Morgan, Immunology News, May, 2008)