Nucleic Acid Detection: Methods and Protocols by Dmitry M. KolpashchikovNucleic Acid Detection: Methods and Protocols by Dmitry M. Kolpashchikov

Nucleic Acid Detection: Methods and Protocols

EditorDmitry M. Kolpashchikov

Hardcover | September 13, 2013

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In Nucleic Acid Chemistry: Methods and Protocols, expert researches in the field detail techniques and approaches for the detection of DNA and RNA. These techniques include the recovery of trace amounts of DNA for amplification and analysis, new qPCR chemistries, new application of isothermal amplification techniques, assays with visual or electric signals for point-of-care diagnostics, improvement of fluorescent in situ hybridization, and new signal amplification techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Nucleic Acid Chemistry: Methods and Protocols seeks to aid scientists in the further study of detection for DNA and RNA.
Title:Nucleic Acid Detection: Methods and ProtocolsFormat:HardcoverDimensions:330 pagesPublished:September 13, 2013Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1627035346

ISBN - 13:9781627035347

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Table of Contents

Part I: Advances in Sample Preparation1. "Getting Blood from a Stone": Ultra-Sensitive Forensic DNA Profiling of Microscopic Bio-particles Recovered from 'Touch DNA' EvidenceErin K. Hanson and Jack Ballantyne 2. Detecting MicroRNA in Human Cancer Tissues with Fluorescence in situ Hybridization Zonggao Shi, Jeff J. Johnson, and M. Sharon Stack Part II: New Real-Time and Instantaneous Assays3. MN Azyme qPCR: A Superior Tool for Multiplex qPCRElisa Mokay and Alison V. Todd4. A New, Multiplex, Quantitative Real-time Polymerase Chain Reaction System for Nucleic Acid Detection and QuantificationFang Liang, Neetika Arora, Kang Liang Zhang, David Che Cheng Yeh, Richard Lai, Darnley Pearson, Graeme Barnett, David Whiley, Theo Sloots, Simon R. Corrie, and Ross T. Barnard 5. Detection of SNP-Containing Human DNA Sequences Using a Split Sensor with Universal Molecular Beacon ReporterYulia V. Gerasimova, Jack Ballantyne, and Dmitry M. Kolpashchikov 6. SNP Analysis using a Molecular Beacon-based Operating Cooperatively (OC) Sensor Evan M. Cornett and Dmitry M. Kolpashchikov  Part III: Isothermal Amplification Methods for Nucleic Acid Detection7. Detection of rpoB Gene Mutations Using Helicase-Dependent AmplificationWanyuan Ao and Robert Jenison          8.      Rapid Detection of Brucella spp. using Loop-mediated Isothermal Amplification (LAMP)Shouyi Chen, Xunde Li, Juntao Li, and Edward R. Atwill 9.       Loop-mediated Isothermal Amplification Method for a Differential Identification of Human Taenia TapewormsYasuhito Sako, Agathe Nkouawa, Tetsuya Yanagida, and Akira Ito 10.   Detection of Mutation by Allele-specific Loop-mediated Isothermal Amplification (AS-LAMP)Hiroka Aonuma, Athanase Badolo , Kiyoshi Okado, and Hirotaka Kanuka  Part IV: Signal Amplification Approac11.      DNA Detection by Cascade Enzymatic Signal Amplification Bingjie Zou, Yinjiao Ma and Guohua Zhou Part V: Non-Fluorescent Detection Formats12.  Visual DNA Detection and SNP Genotyping using Asymmetric PCR and Split DNA EnzymesJia Ling Neo and Mahesh Uttamchandani  13.      Detection of Single-stranded Nucleic Acids via Colorimetric Means, using G-quadruplex ProbesShizuka Nakayama and Herman O. Sintim 14.       Lateral Flow Biosensors for the Detection of Nucleic AcidLingwen Zeng, Puchang Lie, Zhiyuan Fang, Zhuo Xiao 15.     Electrochemiluminescence Detection of c-Myc mRNA in Breast Cancer Cells on a Wireless Bipolar ElectrodeMei-Sheng Wu, Guang-sheng Qian, Jing-Juan Xu, and Hong-Yuan Chen 16.      High-throughput Label-free Detection of DNA Hybridization and Mismatch Discrimination Using Interferometric Reflectance Imaging SensorSunmin Ahn, David S. Freedman, Xirui Zhang, and M. Selim Ünlü1  Part VI: Advances in Fish17.     Graphene -PAMAM Dendrimer -Gold Nano Particle Composite for Electrochemical DNA Hybridization Detection Kumarasamy Jayakumar ,  Rajendiran Rajesh ,Venkataraman Dharuman, and Rangarajan Venkatesan 18.  Application of PNA Openers for Fluorescence-based Detection of Bacterial DNAIrina Smolina 19.  DNA probes for FISH analysis of C-Negative Regions in Human Chromosomes Evgeniy S. Morozkin, Tatyana V. Karamysheva, Pavel P. Laktionov, Valentin V. Vlassov, and Nikolay B. Rubtsov  Part VII: Detection of microRNA20. Quantitative Analysis of MicroRNA in Blood Serum with Protein-Facilitated Affinity Capillary ElectrophoresisNasrin Khan and Maxim V. Berezovski 21. High-Throughput Functional MicroRNA Profiling using Recombinant AAV-Based MicroRNA Sensor Arrays Wenhong Tian, Xiaoyan Dong, Xiaobing Wu, and Zhijian Wu  22.  The Use of Molecular Beacons to Detect and Quantify MicroRNAMeredith B. Baker, Gang Bao, and Charles D. Searles  Part VIII: RNA Imaging in Live Cells23.         Sequence Specific Imaging of Influenza A mRNA in Living Infected Cells Using Fluorescent FIT-PNASusann Kummer, Andrea Knoll, Andreas Herrmann, and Oliver Seitz 24.      Application of Caged Fluorescent Nucleotides to Live-cell RNA ImagingAkimitsu Okamoto