Olfactory Receptors: Methods and Protocols by Chiquito J. CrastoOlfactory Receptors: Methods and Protocols by Chiquito J. Crasto

Olfactory Receptors: Methods and Protocols

EditorChiquito J. Crasto

Hardcover | April 14, 2013

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Despite the best efforts of many and despite landmark discoveries and experimental ingenuity, challenges in the pursuit of research related to olfactory receptors (ORs) continue to exist.  In Olfactory Receptors: Methods and Protocols, experts in the field contribute chapters that serve to address these challenges.  The volume consists of several sections: knowledge dissemination of ORs, theoretical assessments of OR structure and function, as well as development and use of expression systems and experimental functional analysis. Written in the highly successful Methods in Molecular BiologyT series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Concise and practical,  Olfactory Receptors: Methods and Protocols seeks to aid researchers in furthering the knowledge of olfaction and moving us ever closer to the thrilling discoveries that will follow.
Title:Olfactory Receptors: Methods and ProtocolsFormat:HardcoverDimensions:274 pagesPublished:April 14, 2013Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1627033769

ISBN - 13:9781627033763

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Table of Contents

Part I: Knowledge Dissemination of Olfactory Receptors 1. Databases in SenseLab for the Genomics, Proteomics, and Function of Olfactory Receptors            Luis N. Marenco, Gautam Bahl, Lorra Hyland, Jing Shi, Rixin Wang, Peter C. Lai, Perry L. Miller, Gordon M. Shepherd, and Chiquito J. Crasto 2. HORDE: Comprehensive Resource for Olfactory Receptor Genomics            Tsviya Olender, Noam Nativ, and Doron Lancet 3. Identification of Olfactory Receptor Genes from Mammalian Genome Sequences            Yoshihito Niimura Part II: Theoretical Assessments of Olfactory Receptor Structure and Function 4. Molecular Modelling of Odorant / Olfactory Receptor Complexes            Landry Charlier, Jérémie Topin, Peter C. Lai, Chiquito J. Crasto, and Jerome Golebiowski 5. The Electrical Properties of Olfactory Receptors in the Development of Biological Smell Sensors            Eleanora Alfinito,  Jeremy Pousset, and Lino Reggiani 6. A Hit Map-Based Statistical Method to Predict Best Ligands for Orphan Olfactory Receptors: Natural Key Odorants Versus 'Lock Picks'            Dietmar Krautwurst and Matthias Kotthoff Part III: Expression Systems for Olfactory Receptors 7. A Robust, Rapid, and Simple Method of Producing Olfactory Receptors Using Commercial E. coli Cell-Free Systems            Karolina Corin, Brian Cook, and Shuguang Zhang 8. Human Olfactory Receptors: Recombinant Expression in the Baculovirus / Sf9 Insect Cell System, Functional Characterization, and Odorant Identification            Valéry Matarazzo and Catherine Ronin 9. Single Cell RT-PCR Identification of Odorant Receptors Expressed by Olfactory Neurons            Bettina Malnic 10. Next-Generation Sequencing of the Human Olfactory Receptors            Joel D. Mainland, Jason R. Willer, Hiroaki Matsunami, and Nicholas Katsanis 11. Deciphering Activation of Olfactory Receptors Using Heterologous Expression in Saccharomyces cerevisiae and Bioluminescence Resonance Energy Transfer (BRET)            Guenhaël Sanz and Edith Pajot-Augy 12. Olfactory Receptors in Human Airway Epithelia            Xiaoling Gu and Yehuda Ben-Shahar Part IV: Functional Analysis of Olfactory Receptors 13. Functional Characterization of Insect Olfactory Receptor Neurons Through In Vivo Approaches            Michel Renou and Philippe Lucas 14. Functional Assay of Mammalian and Insect Olfactory Receptors Using Xenopus Oocytes            Charles W. Luetje, Andrew S. Nichols, Ana Castro, and Benjamin L. Sherman 15. Pharmacology of Mammalian Olfactory Receptors            Richard S. Smith, Zita Peterlin, and Ricardo C. Araneda 16. Visualizing Olfactory Receptor Expression and Localization in Drosophila            Michael Saina and Richard Benton 17. Deorphanization of Human Olfactory Receptors by Luciferase and Ca-Imaging Methods            Kaveh A. Baghaei Part V: Olfactory Receptor Evolution 18. Evolution of Olfactory Receptors            Kara C. Hoover