Oligonucleotide Synthesis: Methods and Applications by Piet HerdewijnOligonucleotide Synthesis: Methods and Applications by Piet Herdewijn

Oligonucleotide Synthesis: Methods and Applications

EditorPiet Herdewijn

Paperback | November 10, 2010

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A collection of powerful new techniques for oligonucleotide synthesis and for the use of modified oligonucleotides in biotechnology. Among the protocol highlights are a novel two-step process that yields a high purity, less costly, DNA, the synthesis of phosphorothioates using new sulfur transfer agents, the synthesis of LNA, peptide conjugation methods to improve cellular delivery and cell-specific targeting, and triple helix formation. The applications include using molecular beacons to monitor the PCR amplification process, nuclease footprinting to study the sequence-selective binding of small molecules of DNA, nucleic acid libraries, and the use of small interference RNA (siRNA) as an inhibitor of gene expression.
Title:Oligonucleotide Synthesis: Methods and ApplicationsFormat:PaperbackDimensions:452 pages, 9.02 × 5.98 × 0 inPublished:November 10, 2010Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617374415

ISBN - 13:9781617374418

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Table of Contents

Synthesis of DNA Using a New Two-Step CycleDouglas J. Dellinger, Jason R. Betley, Tadeusz K. Wyrzykiewicz, and Marvin H. CaruthersRNA Synthesis Using 2'-O-(Tert-Butyldimethylsilyl) ProtectionBrian S. SproatRNA Oligonucleotide Synthesis Via 5'-Silyl-2'-Orthoester ChemistryStephanie A. Hartsel, David E. Kitchen, Stephen A. Scaringe, and William S. MarshallDimethylthiarum Disulfide: New Sulfur Transfer Reagent in Phosphorothioates Oligonucleotide SynthesisZhiwei Wang, Quanlai Song, and Yogesh S. SanghviAssay for Evaluating Ribonuclease H-Mediated Degradation of RNA-Antisense Oligonucleotide DuplexesAnnie Galarneau, Kyung-Lyum Min, Maria M. Mangos, and Masad J. DamhaDi- and Oligonucleotide Synthesis Using H-Phosphonate ChemistryJacek Stawinski and Roger StrömbergSolid-Phase Synthesis of Circular OligonucleotidesEnrique Pedroso, Nuria Escaja, Miriam Frieden, and Anna GrandasLocked Nucleic Acid SynthesisHenrik M. Pfundheller, Anders M. Sørensen, Christian Lomholt, Anders M. Johansen, Troels Koch, and Jesper WengelSynthesis of DNA Mimics Representing HypNA-pPNA Hetero-oligomersVladimir A. Efimov and Oksana G. ChakhmakhchevaBase-Modified Oligonucleotides With Increased Duplex Stability: Pyrazolo[3,4-d]pyrimidines Replacing PurinesFrank Seela, Yang He, Junlin He, Georg Becher, Rita Kröschel, Matthias Zulauf, and Peter LeonardIntroduction of Hypermodified Nucleotides in RNADarrell R. Davis and Ashok C. BajjiChemical Methods for Peptide-Oligonucleotide Conjugate SynthesisDmitry A. Stetsenko and Michael J. GaitBiotin-Labeled Oligonucleotides With Extraordinarily Long Tethering ArmsA. Michael Morocho, Valeri Karamyshev, Olga Shcherbinina, and Nikolai PolushinUniversal Labeling Chemistry for Nucleic Acid Detection on DNA ChipsAli Laayoun, Eloy Bernal-Méndez, Isabelle Sothier, Mitsuharu Kotera, and Alain TroeschPostsynthetic Functionalization of Triple Helix-Forming OligonucleotidesAlexandre S. Boutorine, and Jian-Sheng SunFluorescence-Based On-Line Detection as an Analytical Tool in RNA ElectrophoresisUte Scheffer and Michael GöbelDesign and Optimization of Molecular Beacon Real-Time Polymerase Chain Reaction AssaysJacqueline A. M. Vet and Salvatore A. E. MarrasHigh-Throughput Production of Optimized Primers (Fimers) for Whole-Genome Direct SequencingNikolai Polushin, Andrei Malykh, A. Michael Morocho, Alexei Slesarev, and Sergei KozyavkinNonenzymatic Template-Directed RNA SynthesisMarcus Hey and Michael GöbelDNAse I Footprinting of Small Molecule Binding Sites on DNAChristian Bailly, Jérôme Kluza, Christopher Martin, Thomas Ellis, and Michael J. WaringGene Targeting Using Peptide Nucleic AcidPeter E. NielsenNucleic Acid Library Construction Using Synthetic DNA ConstructsHani S. Zaher and Peter J. UnrauIn Vitro Selection From Combinatorial Nucleic Acid LibrariesAndres JäschkeIn Vitro Selection Procedures for Identifying DNA and RNA Aptamers Targeted to Nucleic Acids and ProteinsEric Dausse, Christian Cazenave, Bernard Rayner, and Jean-Jacques ToulméShort Double-Stranded Ribonucleic Acid as Inhibitor of Gene Expression by the Interference MechanismJean-Rémi Bertrand, Andrei Maksimenko, and Claude MalvyIndex