Opioid Research: Methods and Protocols by Zhizhong Z. PanOpioid Research: Methods and Protocols by Zhizhong Z. Pan

Opioid Research: Methods and Protocols

EditorZhizhong Z. Pan

Paperback | November 9, 2010

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This comprehensive collection of the major cross-disciplinary methods and protocols used in current opioid research covers topics ranging from molecular and genetic techniques, to behavioral analyses of animal models and to clinical practices. The well-practiced authors describe their best molecular techniques for the cloning and expression of opioid receptors, and for the quantitative characterization of their signaling pathways, as well as for mapping the distribution and detecting the expression levels of opioid receptors, opioid peptides, and their messages in both brain tissues and individual cells. Also included are methods for the creation of in vitro and in vivo animal models to study opioid functions, as well as the clinical applications in the treatment of pain and opioid addiction.
Title:Opioid Research: Methods and ProtocolsFormat:PaperbackDimensions:308 pages, 9.02 × 5.98 × 0 inPublished:November 9, 2010Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:161737329X

ISBN - 13:9781617373299

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Table of Contents

Part I. Molecular Characterization of Opioid Receptors and Signaling PathwaysMolecular Cloning of Opioid Receptors by cDNA Library ScreeningYing-Xian PanExpression of Opioid Receptors in Mammalian Cell LinesYing-Xian PanAssessing Opioid Regulation of Adenylyl Cyclase Activity in Intact CellsDeepak R. Thakker, Hatice Z. Ozsoy, and Kelly M. StandiferAnalysis of Opioid-Induced Kinase Activation, Lan MaStudy of Opioid Receptor Phosphorylation Using Cell-Labeling Method with 32P-OrthrophosphateJia Bei WangOpioid Receptor Coupling to GIRK Channels: In Vitro Studies Using a Xenopus Oocyte Expression System and In Vivo Studies on Weaver Mutant MiceKazutaka Ikeda, Mitsunobu Yoshii, Ichiro Sora, and Toru KobayashiIdentification of Alternatively Spliced Variants from Opioid Receptor GenesYing-Xian PanPart II. Mapping and Detection of Endogenous OpioidsImmunohistochemical Localization of m-, d- and k-Opioid Receptors Within the Antinociceptive Brainstem CircuitsAlexander E. KalyuzhnyIn Situ Hybridization in Neural TissuesHoward B. GutsteinQuantitative Single-Cell RT-PCR for Opioid Receptors and Housekeeping GenesSeth C. SilbertGene Arrays and Proteomics: A PrimerLionel Moulédous and Howard B. GutsteinPart III. Model Systems for Studies on Opioid FunctionsOpioid Receptor Oligomerization: Detection and Functional Characterization of Interacting ReceptorsIvone Gomes, Julija Filipovska, and Lakshmi A. DeviRecombinant Opioid Receptors: Structure-Function RelationshipJulija Filipovska, Ivone Gomes, Wei Xu, Chongguang Chen, Lee-Yuan Liu-Chen, and Lakshmi A. DeviReceptor Knock-Out and Gene Targeting: Generation of Knock-Out MiceIchiro Sora, Kazutaka Ikeda, and Yuji MishinaPharmacological Interventions at the Spinal Cord: Intrathecal InjectionsDaphné A. Robinson and Min ZhuoOpioid Tolerance in Adult and Neonatal RatsZhizhong Z. PanAnimal Models of Neuropathic PainWilliam J. Martin, Laike St. A. Stewart, and Jason W. TarpleyPlace Conditioning to Study Drug Reward and AversionWilliam A. Carlezon, Jr.Opiate Self-AdministrationZheng-Xiong Xi and Elliot A. SteinPart IV. Clinical Applications of OpioidsAcute, Chronic, and Cancer Pain: Clinical ManagementAllen W. BurtonClinical Treatment of Opioid Addiction and DependenceWalter Ling, Richard A. Rawson and Margaret ComptonIndex