Osteoporosis: Methods and Protocols by Jennifer WestendorfOsteoporosis: Methods and Protocols by Jennifer Westendorf

Osteoporosis: Methods and Protocols

EditorJennifer Westendorf

Paperback | November 3, 2014

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In recent years, the study of bone cells and tissues at the cellular and molecular levels in a variety of models has revolutionized the field. In Osteoporosis: Methods and Protocols, leading scientists from around the world share their step-by-step laboratory protocols for studying bone biology. The topics covered in this volume include in vitro models, in vivo models utilized for drug testing, tissue engineering and osteoporosis studies in either gender, state-of-the-art molecular techniques to assess single genes or for global genomic analysis, powerful imaging techniques, and many more. As a volume in the highly successful Methods in Molecular BiologyT series, each chapter provides a brief introduction, a list of necessary materials, and a Notes section detailing tips on troubleshooting and avoiding known pitfalls.

Comprehensive and cutting-edge, Osteoporosis: Methods and Protocols is the perfect guide for both new and experienced experimentalists attempting to study the devastating silent disease known as osteoporosis.

Title:Osteoporosis: Methods and ProtocolsFormat:PaperbackPublished:November 3, 2014Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1627038477

ISBN - 13:9781627038478

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Table of Contents

Table of Contents(* indicates corresponding author) In vitro models1. Isolation and Culture of Rodent Osteoprogenitor CellsThomas A. Owen* and Lydia C. Pan2. Osteoclast Culture and Resorption AssaysElizabeth W. Bradley and Merry Jo Oursler*3. Assessing New Bone Formation in Neonatal Calvarial Organ CulturesKhalid S. Mohammad, John M. Chirgwin and Theresa Guise*4. Detection of Apoptosis of Bone Cells In VitroTeresita Bellido*and Lilian I. Plotkin In vivo rodent models5. In Vivo Parathyroid Hormone Treatments and mRNA Isolation and AnalysisXin Li, Ling Qin and Nicola C. Partridge*6. Assessment of Bone Formation Capacity Using In Vivo Transplantation Assays: Procedure and Tissue AnalysisBadem M. Abdalleh, Nicholas Ditzel and Moustapha Kassem*7. Ossicle and Vossicle Implant Model SystemsGlenda J. Pettway and Laurie K. McCauley*8. Utility of the Ovariectomized (OVX) Rat as a Model for Human Osteoporosis In Drug DiscoveryYogendra P. Kharode*, Michael C. Sharp and Peter V. N. Bodine 9. Orchidectomy (ORX) Models of OsteoporosisStéphane Blouin, Hélène Libouban, Marie Françoise Moreau and Daniel Chappard* Methods of gene delivery10. Gene Delivery by AdenovirusesRenny Franceschi* and Chunxi Ge11. Lentiviral Delivery of shRNA Constructs into Osteoblasts Marc N. Wein, Dallas C. Jones and Laurie H. Glimcher*12. Gene Delivery by RetrovirusesValerie Deregowski and Ernesto Canalis*Molecular and GeneticTechniques13. Chromatin Immunoprecipitation (ChIP) Assays: Application of ChIP-on-chip for Defining Dynamic Transcriptional Mechanisms in Bone CellsMargaret van der Deen, Mohammad Q. Hassan, Jitesh Pratap, Nadiya M. Teplyuk, Daniel W. Young, Amjad Javed, Sayyed K. Zaidi, Jane B. Lian, Martin Montecino, Janet L. Stein, Gary S. Stein and Andre J. van Wijnen*14. Identification of Transcription Factor Target Genes by ChIP Display (CD)Artem Barski, Steve Pregizer and Baruch Frenkel*15. Application of Laser Capture Microdissection (LCM) Technique for Molecular Definition of Skeletal Cell Differentiation In VivoDafna Benayahu*, Rina Socher, and Irena Shur16. Quantitative Trait Loci (QTL) MappingDong-Hai Xiong, Jian-Feng Liu, Yan-Fang Guo, Yan Guo, Tie-Lin Yang, Shu-Gui Chen, Hui Jiang, Yuan Chen, Fang Yang, Robert R. Recker and Hong-Wen Deng*Imaging Techniques17. In Situ Nuclear Organization of Regulator MachineryShirwin M. Pockwinse, Sayyed K. Zaidi, Ricardo F. Medina, Rachit Bakshi, Krishna P. Kota, Syed A. Ali, Daniel W. Young, Jeffery A. Nickerson, Amjad Javed, Martin Montecino, Andre J. van Wijnen, Jane B. Lian, Janet L. Stein and Gary S. Stein*18. Bioluminescent Imaging in BoneYoram Zilberman, Yossi Gafni, Gadi Pelled, Zulma Gazit, and Dan Gazit*19. Micro Computed Tomography - A Method for the Nondestructive Evaluation of the Three-Dimensional Structure of Biological SpecimensMartin Stauber and Ralph Müller*20. Fourier Transform Infrared (FT-IR) Microspectroscopy and Microscopic ImagingSamuel Gourion-Arsiquaud, Paul A. West and Adele L. Boskey*Mechanical