Pancreatic Cancer: Methods and Protocols by Gloria H SuPancreatic Cancer: Methods and Protocols by Gloria H Su

Pancreatic Cancer: Methods and Protocols

EditorGloria H Su

Hardcover | January 31, 2013

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Pancreatic ductal adenocarcinoma is the fifth leading cause of cancer death in the USA.  Pancreatic cancer develops as a result of the accumulation of genetic and epigenetic alterations in cancer-causing genes such as oncogenes and tumor-suppressor genes.  The second edition of Pancreatic Cancer: Methods and Protocols provides a broad range of protocols for molecular, cellular, pathological, and statistical analyses of sporadic and familial pancreatic cancer.   It covers topics from in-vitro cell cultures to in-vivo mouse models, DNA to protein manipulation, and genetic and epigenetic analyses to treatment development. Written in the highly successful Methods in Molecular BiologyT series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, written by highly renowned investigators with expertise in pancreatic cancer, Pancreatic Cancer: Methods and Protocols, Second Edition is an invaluable source of proven protocols to those who are interested in joining the fight against pancreatic cancer.
Title:Pancreatic Cancer: Methods and ProtocolsFormat:HardcoverDimensions:401 pagesPublished:January 31, 2013Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:162703286X

ISBN - 13:9781627032865

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Table of Contents

1. Identification and Analysis of Precursors to Invasive Pancreatic CancerHanno Matthaei, Marco Dal Molin, Anirban Maitra,  2. Tissue Microarrays: Construction and UseHelen Remotti  3. Immunohistochemistry of Pancreatic NeoplasiaSukhwinder Kaur, Tomohiro Shimizu, Michael J. Baine, Sushil Kumar and Surinder K. Batra 4. A Method for Conducting Highly Sensitive microRNA in situ Hybridization and Immunohistochemical Analysis in Pancreatic Cancer Lorenzo F. Sempere, and Murray Korc 5. Optimal Molecular Profiling of Tissue and Tissue Components: Defining the Best Processing and Microdissection Methods for Biomedical ApplicationsJaime Rodriguez-Canales, Jeffrey C. Hanson, Jason D. Hipp, Ulysses J. Balis,  Michael A. Tangrea, Michael R. Emmert-Buck, and G. Steven Bova  6. Considerations for Sequencing Analyses of Pancreatic Cancer Progression and MetastasisAlvin Makohon-Moore and Christine A. Iacobuzio-Donahue 7. DNA Methylation Analysis in Human CancerEileen O'Sullivan, Michael Goggins 8. Quantitative Real-Time PCR Expression Analysis of Peripheral Blood Mononuclear Cells in Pancreatic Cancer PatientsMichael J. Baine, Kavita Mallya, and Surinder K. Batra 9. Development of a Cytokine-modified Allogeneic Whole Cell Pancreatic Cancer VaccineDan Laheru, Barbara Biedrzycki, and Elizabeth M. Jaffee 10. The Prevention and Genetics of Pancreatic Cancer - A Programmatic ApproachAimee L Lucas, Michelle M Chang, Marla D Lipsyc, and Harold Frucht 11. Development of Orthotopic Pancreatic Tumor Mouse ModelsWanglong Qiu, Gloria H. Su 12. Evaluating Dietary Compounds in Pancreatic Cancer Modeling SystemsEmman Mascariñas, Guido Eibl , Paul J. Grippo 13. Quantification of Murine Pancreatic Tumors by High Resolution UltrasoundStephen A. Sastra and Kenneth P. Olive 14. Microdissection and Culture of Murine Pancreatic Ductal Epithelial CellsYuliya Pylayeva-Gupta, Kyoung Eun Lee and Dafna Bar-Sagi15. Sphere-forming Assays for Assessment of Benign and Malignant Pancreatic Stem CellsJulia Wang, Jennifer M. Bailey, Meritxell Rovira, Steven D. Leach 16. Analysis of Transplanted Bone Marrow Derived Cells in Chronic PancreatitisChristoph B. Westphalen, Frederic Marrache, Timothy C. Wang 17. Analysis of the Potential for Pancreatic Cancer Metastasis In Vitro and In VivoChen Huang and Keping Xie  18. The use of Fluorescent Probes in the Study of Reactive Oxygen Species in Pancreatic Cancer CellsRichard D. Dinnen, Yuehua Mao,  and Robert L. Fine 19. Analysis of Tumor Associated Mucin Glycotopes by Western Transfer MethodsVinayaga S. Gnanapragassam, Maneesh Jain, Satyanaraya Rachagani, Moorthy P. Ponnusamy, and Surinder K Batra 20. Evaluating Tumor AngiogenesisMinji Kim, Jessica Kandel, Jan Kitajewski 21. Pooled Genome-wide RNAi ScreeningsRuth Rodriguez-Barrueco, Netonia Marshall, Jose M. Silva 22. Pooled ShRNA screenings: Computational Analysis Jiyang Yu, Preeti Putcha, Andrea Califano, Jose M. Silva 4. A Method for Conducting Highly Sensitive microRNA in situ Hybridization and Immunohistochemical Analysis in Pancreatic Cancer Lorenzo F. Sempere, and Murray Korc 5. Optimal Molecular Profiling of Tissue and Tissue Components: Defining the Best Processing and Microdissection Methods for Biomedical ApplicationsJaime Rodriguez-Canales, Jeffrey C. Hanson, Jason D. Hipp, Ulysses J. Balis,  Michael A. Tangrea, Michael R. Emmert-Buck, and G. Steven Bova  6. Considerations for Sequencing Analyses of Pancreatic Cancer Progression and MetastasisAlvin Makohon-Moore and Christine A. Iacobuzio-Donahue 7. DNA Methylation Analysis in Human CancerEileen O'Sullivan, Michael Goggins 8. Quantitative Real-Time PCR Expression Analysis of Peripheral Blood Mononuclear Cells in Pancreatic Cancer PatientsMichael J. Baine, Kavita Mallya, and Surinder K. Batra 9. Development of a Cytokine-modified Allogeneic Whole Cell Pancreatic Cancer VaccineDan Laheru, Barbara Biedrzycki, and Elizabeth M. Jaffee 10. The Prevention and Genetics of Pancreatic Cancer - A Programmatic ApproachAimee L Lucas, Michelle M Chang, Marla D Lipsyc, and Harold Frucht 11. Development of Orthotopic Pancreatic Tumor Mouse ModelsWanglong Qiu, Gloria H. Su 12. Evaluating Dietary Compounds in Pancreatic Cancer Modeling SystemsEmman Mascariñas, Guido Eibl , Paul J. Grippo 13. Quantification of Murine Pancreatic Tumors by High Resolution UltrasoundStephen A. Sastra and Kenneth P. Olive 14. Microdissection and Culture of Murine Pancreatic Ductal Epithelial CellsYuliya Pylayeva-Gupta, Kyoung Eun Lee and Dafna Bar-Sagi15. Sphere-forming Assays for Assessment of Benign and Malignant Pancreatic Stem CellsJulia Wang, Jennifer M. Bailey, Meritxell Rovira, Steven D. Leach 16. Analysis of Transplanted Bone Marrow Derived Cells in Chronic PancreatitisChristoph B. Westphalen, Frederic Marrache, Timothy C. Wang 17. Analysis of the Potential for Pancreatic Cancer Metastasis In Vitro and In VivoChen Huang and Keping Xie  18. The use of Fluorescent Probes in the Study of Reactive Oxygen Species in Pancreatic Cancer CellsRichard D. Dinnen, Yuehua Mao,  and Robert L. Fine 19. Analysis of Tumor Associated Mucin Glycotopes by Western Transfer MethodsVinayaga S. Gnanapragassam, Maneesh Jain, Satyanaraya Rachagani, Moorthy P. Ponnusamy, and Surinder K Batra 20. Evaluating Tumor AngiogenesisMinji Kim, Jessica Kandel, Jan Kitajewski 21. Pooled Genome-wide RNAi ScreeningsRuth Rodriguez-Barrueco, Netonia Marshall, Jose M. Silva 22. Pooled ShRNA screenings: Computational Analysis Jiyang Yu, Preeti Putcha, Andrea Califano, Jose M. Silva 9. Development of a Cytokine-modified Allogeneic Whole Cell Pancreatic Cancer VaccineDan Laheru, Barbara Biedrzycki, and Elizabeth M. Jaffee 10. The Prevention and Genetics of Pancreatic Cancer - A Programmatic ApproachAimee L Lucas, Michelle M Chang, Marla D Lipsyc, and Harold Frucht 11. Development of Orthotopic Pancreatic Tumor Mouse ModelsWanglong Qiu, Gloria H. Su 12. Evaluating Dietary Compounds in Pancreatic Cancer Modeling SystemsEmman Mascariñas, Guido Eibl , Paul J. Grippo 13. Quantification of Murine Pancreatic Tumors by High Resolution UltrasoundStephen A. Sastra and Kenneth P. Olive 14. Microdissection and Culture of Murine Pancreatic Ductal Epithelial CellsYuliya Pylayeva-Gupta, Kyoung Eun Lee and Dafna Bar-Sagi15. Sphere-forming Assays for Assessment of Benign and Malignant Pancreatic Stem CellsJulia Wang, Jennifer M. Bailey, Meritxell Rovira, Steven D. Leach 16. Analysis of Transplanted Bone Marrow Derived Cells in Chronic PancreatitisChristoph B. Westphalen, Frederic Marrache, Timothy C. Wang 17. Analysis of the Potential for Pancreatic Cancer Metastasis In Vitro and In VivoChen Huang and Keping Xie  18. The use of Fluorescent Probes in the Study of Reactive Oxygen Species in Pancreatic Cancer CellsRichard D. Dinnen, Yuehua Mao,  and Robert L. Fine 19. Analysis of Tumor Associated Mucin Glycotopes by Western Transfer MethodsVinayaga S. Gnanapragassam, Maneesh Jain, Satyanaraya Rachagani, Moorthy P. Ponnusamy, and Surinder K Batra 20. Evaluating Tumor AngiogenesisMinji Kim, Jessica Kandel, Jan Kitajewski 21. Pooled Genome-wide RNAi ScreeningsRuth Rodriguez-Barrueco, Netonia Marshall, Jose M. Silva 22. Pooled ShRNA screenings: Computational Analysis Jiyang Yu, Preeti Putcha, Andrea Califano, Jose M. Silva15. Sphere-forming Assays for Assessment of Benign and Malignant Pancreatic Stem CellsJulia Wang, Jennifer M. Bailey, Meritxell Rovira, Steven D. Leach 16. Analysis of Transplanted Bone Marrow Derived Cells in Chronic PancreatitisChristoph B. Westphalen, Frederic Marrache, Timothy C. Wang 17. Analysis of the Potential for Pancreatic Cancer Metastasis In Vitro and In VivoChen Huang and Keping Xie  18. The use of Fluorescent Probes in the Study of Reactive Oxygen Species in Pancreatic Cancer CellsRichard D. Dinnen, Yuehua Mao,  and Robert L. Fine 19. Analysis of Tumor Associated Mucin Glycotopes by Western Transfer MethodsVinayaga S. Gnanapragassam, Maneesh Jain, Satyanaraya Rachagani, Moorthy P. Ponnusamy, and Surinder K Batra 20. Evaluating Tumor AngiogenesisMinji Kim, Jessica Kandel, Jan Kitajewski 21. Pooled Genome-wide RNAi ScreeningsRuth Rodriguez-Barrueco, Netonia Marshall, Jose M. Silva 22. Pooled ShRNA screenings: Computational Analysis Jiyang Yu, Preeti Putcha, Andrea Califano, Jose M. Silva 20. Evaluating Tumor AngiogenesisMinji Kim, Jessica Kandel, Jan Kitajewski 21. Pooled Genome-wide RNAi ScreeningsRuth Rodriguez-Barrueco, Netonia Marshall, Jose M. Silva 22. Pooled ShRNA screenings: Computational Analysis Jiyang Yu, Preeti Putcha, Andrea Califano, Jose M. Silva 21. Pooled Genome-wide RNAi ScreeningsRuth Rodriguez-Barrueco, Netonia Marshall, Jose M. Silva 22. Pooled ShRNA screenings: Computational Analysis Jiyang Yu, Preeti Putcha, Andrea Califano, Jose M. Silva 22. Pooled ShRNA screenings: Computational Analysis Jiyang Yu, Preeti Putcha, Andrea Califano, Jose M. Silva