PCR 1: A Practical Approach

Paperback | July 1, 1992

EditorM. J. McPherson, P. Quirke, G. R. Taylor

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The Polymerase Chain Reaction (PCR) has become one of the most widely used techniques in biomedical research, enabling the fast, inexpensive production of large quantities of DNA from minute amounts of starting material. Although the basic procedure is very simple, many variations of it havebeen developed for different applications, for use in such diverse areas as forensic and diagnostic medicine, genetic engineering, and the food industry.PCR: A Practical Approach provides a general introduction to PCR for researchers new to the area, followed by chapters detailing specific applications of the technique. It covers gene analysis at the level of restriction enzyme polymorphism, point mutation and sequence analysis. Methods for usingdifferent source materials - cloned DNA, genomic DNA, RNA, nucleic acid from archive material and PCR products themselves - are given.The emphasis throughout is on the practical applications of the technique and detailed experimental protocols are included at all stages, backed up by advice on avoiding potential pitfalls. This volume provides a starting point for those new to PCR and will also serve as a useful reference for moreexperienced researchers in all areas of biological science.

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The Polymerase Chain Reaction (PCR) has become one of the most widely used techniques in biomedical research, enabling the fast, inexpensive production of large quantities of DNA from minute amounts of starting material. Although the basic procedure is very simple, many variations of it havebeen developed for different applications, fo...

M.J. McPherson and P. Quirke are both at the University of Leeds. G.R. Taylor is with the Yorkshire Regional DNA Laboratory.

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Format:PaperbackDimensions:274 pages, 9.25 × 6.3 × 0.59 inPublished:July 1, 1992Publisher:Oxford University Press

The following ISBNs are associated with this title:

ISBN - 10:0199631964

ISBN - 13:9780199631964

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'Concise, well written and interesting, I would thoroughly recommend this text as a useful addition to the PCR library.'Soo Pickering, University of Cambridge, Trends in Biochemical Sciences, Volume 17, No. 5, May 1992