Peptide Antigens: A Practical Approach: A Practical Approach

Paperback | October 1, 1994

EditorG. Brian Wisdom

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Peptide antigens and their antibodies have been widely exploited for the measurement, location, and purification of oligopeptides such as peptide hormones. Recently, the use of peptides to mimic substructures of proteins has led to major expansion in the application of anti-peptide antibodies. These have been especially important in identifying the characterizing proteins which are only known by their primary structure derived from a DNA sequence. Another area of recent expansion is the use of defined peptided antigens to identify and map antibodies and T-cell receptors. This book provides information and detailed protocols for the main techniques for exploitation of peptide antigens and anti-peptide antibodies, and will interest research workers in many fields.

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Peptide antigens and their antibodies have been widely exploited for the measurement, location, and purification of oligopeptides such as peptide hormones. Recently, the use of peptides to mimic substructures of proteins has led to major expansion in the application of anti-peptide antibodies. These have been especially important in id...

G. Brian Wisdom, Division of Biochemistry, Medical Biology Centre, The Queen's University, Belfast.

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Format:PaperbackDimensions:272 pages, 9.21 × 6.14 × 0.01 inPublished:October 1, 1994Publisher:Oxford University PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:0199634513

ISBN - 13:9780199634514

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Table of Contents

1. Peptide antigens and anit-peptide antibodies2. Epitope predictions from the primary structure of proteins3. Solid-phase peptide synthesis4. Immunization with peptide carrier complexes: traditional and multiple-antigen peptide systems5. Preparative immunoaffinity techniques6. Immunoassays7. Epitope mapping using synthetic peptides8. Epitope mapping using libraries of random peptides displayed on phageAppendix 2: The DNA-encoded amino acids

Editorial Reviews

`This volume is another fine addition to The Practical Approach Series ... the book lives up to the consistently high quality of this series ... useful small volume which deserves to become a good companion to the scientist at the bench.'Hans Rudolf Bosshard, FEBS Letters 369 (1995)