Peptide Research Protocols: Endothelin by Janet J. MaguirePeptide Research Protocols: Endothelin by Janet J. Maguire

Peptide Research Protocols: Endothelin

EditorJanet J. Maguire, Anthony P. Davenport

Paperback | November 10, 2010

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A panel of multidisciplinary experts describes in detail readily reproducible methods to investigate all aspects of the endothelin system from its synthesis and metabolism, to its function in health and disease. Theses methods use state-of-the-art molecular techniques to quantify the expression of mRNA for both endothelin receptors and the endothelin converting enzymes. They show how peptides, precursors, receptors, and synthetic enzymes can be localized and quantified in plasma, culture supernatants, tissue homogenate, and tissue sections using antibodies. Several in vivo protocols illustrate the role of the endothelin peptides in healthy human individuals and describe animal models that can be used to predict the therapeutic potential of cardiovascular drugs that manipulate endothelin synthesis or function.

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Title:Peptide Research Protocols: EndothelinFormat:PaperbackDimensions:252 pagesPublished:November 10, 2010Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617372919

ISBN - 13:9781617372919

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Table of Contents

Part I. Endothelin Peptide ProtocolsImmunocytochemical Localization of Endothelin Peptides, Precursors, and Endothelin-Converting EnzymesRhoda E. KucAnalysis of Endothelins by Enzyme-Linked Immunosorbent Assay and RadioimmunoassayAnthony P. Davenport and Rhoda E. KucSeparation of Endothelin Isoforms by High Performance Liquid Chromatography and Detection by RadioimmunoassayMichael J. AshbyPart II. Endothelin Receptor ProtocolsRadioligand Binding Assays and Quantitative Autoradiography of Endothelin ReceptorsAnthony P. Davenport and Rhoda E. KucUsing Receptor Antagonists in Binding Studies to Characterize a Mammalian Endothelin ReceptorJ. Ruth Wu-WongQuantification of Endothelin Receptor mRNA by Competitive RT-PCRPaula J. W. Smith and Juan Carlos MongePart III. Endothelin-Converting Enzyme ProtocolsDetection and Assessment of Endothelin-Converting Enzyme ActivityCarolyn D. Jackson and Anthony J. TurnerQuantitative Measurement of mRNA Levels by RT-PCR: Studies of ECE-1 IsoformsDelphine M. Lees, Noorafza Q. Khan, Stewart Barker, and Roger CorderEvaluation of Endothelin-Converting Enzyme Inhibitors Using Cultured Cells, Roger CorderEndothelin-Converting Enzyme Activity in Vascular Smooth Muscle Preparations In VitroJanet J. MaguirePart IV. In Vitro Functional AssaysCharacterization of a New Endothelin Receptor Ligand by In Vitro AssaysJ. Ruth Wu-WongUse of In Vitro Organ Cultures of Human Saphenous Vein as a Model for Intimal ProliferationKaren E. PorterPart V. In Vivo Functional AssaysInvestigation of the Endothelin System in Experimental Heart FailureGillian A. Gray and Lorcan SherryLocal Forearm Vasoconstriction to Endothelin-1 Measured by Venous Occlusion Plethysmography In VivoFiona E. Strachan and David J. WebbIndex

Editorial Reviews

"This book is excellent for what it intends to be: to provide a coherent series of protocols on a focused topic. It efficiently integrates biochemical, pharmacological, physiological and molecular biological methods..." -Acta Cardiologica