Phage Display: A Practical Approach by Tim ClacksonPhage Display: A Practical Approach by Tim Clackson

Phage Display: A Practical Approach

EditorTim Clackson, Henry B. Lowman

Paperback | September 23, 2004

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This new book is designed to enable researchers to design and undertake all aspects of a phage display project, from designing an experimental strategy and constructing a library to performing selections and analyzing the results.All of the protocols and chapters are extensivelycross-referenced, allowing readers to move beyond the specific examples provided in order to customize the procedures for their own protein or selection system of interest. Phage Display is an up-to-date, comprehensive and integrated experimental guide to the technique, which is essential readingfor anyone currently using, or wishing to use the technique for basic research and drug discovery.

About The Author

Dr Tim Clackson Vice President, Gene Therapy and Genomics ARIAD Pharmaceuticals, Inc., 26 Landsdowne Street, Cambridge, MA 02139, USA +1 617 494 0400 ext. 258 +1 617 225 2589 clackson@ariad.com Tim Clackson, Ph.D. is Senior Vice President, Science and Technology at ARIAD Pharmaceuticals, Inc., where he has served for eight years ...
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Title:Phage Display: A Practical ApproachFormat:PaperbackDimensions:360 pages, 9.69 × 7.44 × 0.76 inPublished:September 23, 2004Publisher:Oxford University PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:019963873X

ISBN - 13:9780199638734

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Table of Contents

1. Russel, Lowman and Clackson: Introduction to phage biology and phage display2. Sidhu and Weiss: Constructing phage display libraries by oligonucleotide-directed mutagenesis3. Miyazaki and Arnold: In vitro DNA recombination4. Dennis and Lowman: Phage selection strategies for improved affinity and specificity of proteins and peptides5. DeLano and Cunningham: Rapid screening of phage displayed protein binding affinities by phage ELISA6. Cwirla et al: Identification of novel ligands for receptors using recombinant peptide libraries7. Matthews and Ballinger: Substrate phage display8. Tuna et al: Protease-based selection of stably folded proteins and protein domains from phage display libraries9. Isalan and Choo: Phage display of zinc fingers and other nucleic acid-binding motifs10. Hoffman et al: In vivo and ex vivo selections using phage-displayed libraries11. Monaci and Cortese: Screening phage libraries with sera12. Jespers and Fransen: Interaction cloning using cDNA libraries displayed on phage13. Bradbury and Marks: Phage antibody libraries14. Neilson and Marks: Affinity maturation of phage antibodies