Phage Display: A Practical Approach

Paperback | September 23, 2004

EditorTim Clackson, Henry B. Lowman

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This new book is designed to enable researchers to design and undertake all aspects of a phage display project, from designing an experimental strategy and constructing a library to performing selections and analyzing the results.All of the protocols and chapters are extensivelycross-referenced, allowing readers to move beyond the specific examples provided in order to customize the procedures for their own protein or selection system of interest. Phage Display is an up-to-date, comprehensive and integrated experimental guide to the technique, which is essential readingfor anyone currently using, or wishing to use the technique for basic research and drug discovery.

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This new book is designed to enable researchers to design and undertake all aspects of a phage display project, from designing an experimental strategy and constructing a library to performing selections and analyzing the results.All of the protocols and chapters are extensivelycross-referenced, allowing readers to move beyond the spec...

Dr Tim Clackson Vice President, Gene Therapy and Genomics ARIAD Pharmaceuticals, Inc., 26 Landsdowne Street, Cambridge, MA 02139, USA +1 617 494 0400 ext. 258 +1 617 225 2589 clackson@ariad.com Tim Clackson, Ph.D. is Senior Vice President, Science and Technology at ARIAD Pharmaceuticals, Inc., where he has served for eight years ...

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Format:PaperbackDimensions:360 pages, 9.69 × 7.44 × 0.76 inPublished:September 23, 2004Publisher:Oxford University PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:019963873X

ISBN - 13:9780199638734

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Table of Contents

1. Russel, Lowman and Clackson: Introduction to phage biology and phage display2. Sidhu and Weiss: Constructing phage display libraries by oligonucleotide-directed mutagenesis3. Miyazaki and Arnold: In vitro DNA recombination4. Dennis and Lowman: Phage selection strategies for improved affinity and specificity of proteins and peptides5. DeLano and Cunningham: Rapid screening of phage displayed protein binding affinities by phage ELISA6. Cwirla et al: Identification of novel ligands for receptors using recombinant peptide libraries7. Matthews and Ballinger: Substrate phage display8. Tuna et al: Protease-based selection of stably folded proteins and protein domains from phage display libraries9. Isalan and Choo: Phage display of zinc fingers and other nucleic acid-binding motifs10. Hoffman et al: In vivo and ex vivo selections using phage-displayed libraries11. Monaci and Cortese: Screening phage libraries with sera12. Jespers and Fransen: Interaction cloning using cDNA libraries displayed on phage13. Bradbury and Marks: Phage antibody libraries14. Neilson and Marks: Affinity maturation of phage antibodies