Plant Cell Biology: A Practical Approach

Paperback | May 15, 2001

EditorChris Hawes, Beatrice Satiat-Jeunemaitre

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With the 'post genomics' era comes an increasing demand for the techniques of cell biology, critical to interpreting the function and location of the cell's myriad proteins and macromolecules. In response, this second edition of Plant Cell Biology balances established techniques, includingclassical histochemistry and electron microscopy, with new developments in the field. The book covers a substantial range of methods for working on living cells, including the application of fluorescent probes, cytometry, expression systems, the use of green fluorescent protein, micromanipulationand electrophysiological techniques. Also featured are chapters on macromolecular location procedures involving immunocytochemistry and in situ hybridisation, and the book concludes with a range of biochemical techniques for the isolation of cytoplasmic organelles. The book provides advancedstudents, postgraduates and researchers in the plant sciences with an invaluably comprehensive guide to the ever-growing field of plant cell biology.

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From the Publisher

With the 'post genomics' era comes an increasing demand for the techniques of cell biology, critical to interpreting the function and location of the cell's myriad proteins and macromolecules. In response, this second edition of Plant Cell Biology balances established techniques, includingclassical histochemistry and electron microsco...

Chris Hawes is at Oxford Brookes University. Beatrice Satiat-Jeunemaitre is at Institut des Sciences Vegetales, Gif-sur-Yvette.

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Format:PaperbackPublished:May 15, 2001Publisher:Oxford University PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:0199638659

ISBN - 13:9780199638659

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Table of Contents

Introduction to optical microscopy for plant cell biologyFluorescent probes for living plant cellsFlow cytometryTransient expression, a tool to address questions in plant cell biologyThe green fluorescent protein (GFP) as reporter in plant cellsMicroinjectionMicromanipulation by laser microbeam and optical tweezersElectrophysiological methods: monitoring exo- and endocytosis in real timePlant histologyImmunocytochemistry for light microscopyElectron microscopyIn Situ hybridizationOrganelle isolation