Plant Tissue Culture: Techniques and Experiments

Other | October 22, 2012

byRoberta H. Smith, Roberta H. Smith

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Plant Tissue Culture,Third Edition builds on the classroom tested, audience proven manual that has guided users through successful plant culturing A.tumefaciens mediated transformation, infusion technology, the latest information on media components and preparation, and regeneration and morphogenesis along with new exercises and diagrams provide current information and examples.

The included experiments demonstrate major concepts and can be conducted with a variety of plant material that are readily available throughout the year. This book provides a diverse learning experience and is appropriate for both university students and plant scientists.



  • Provides new exercises demonstrating tobacco leaf infiltration to observe transient expression of proteins and subcellular location of the protein, and information on development of a customized protocol for protoplast isolation for other experimental systems
  • Includes detailed drawings that complement both introductions and experiments
  • Guides reader from lab setup to supplies, stock solution and media preparation, explant selection and disinfestations, and experimental observations and measurement
  • Provides the latest techniques and media information, including A. tumefaciens mediated transformation and infusion technology
  • Fully updated literature

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From the Publisher

Plant Tissue Culture,Third Edition builds on the classroom tested, audience proven manual that has guided users through successful plant culturing A.tumefaciens mediated transformation, infusion technology, the latest information on media components and preparation, and regeneration and morphogenesis along with new exercises and diagra...

Format:OtherDimensions:208 pages, 1 × 1 × 1 inPublished:October 22, 2012Publisher:Academic PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:0124159850

ISBN - 13:9780124159853

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Extra Content

Table of Contents

1. History of Plant Cell Culture

Trevor A. Thorpe

2. Setup of a Tissue Culture Laboratory

3. Media Components and Preparation

4. Explant Preparation

5. Contamination

6. Callus Induction

7. Regeneration and Morphogenesis

8. Woody Shrubs and Trees

Brent H. McCown

9. Haploid Plants from Anther Culture

10. Embryo Rescue

11. Meristem Culture for Virus-Free Plants

12.In VitroPropagation for Commercial Production of Ornamentals

13. Protoplast Isolation and Fusion

Jungeun Kim Park, Sunghun Park, & James E. Craven

14. Agrobacterium-Mediated Transformation of Plants

Jungeun Kim Park, Sunghun Park, Qingyu Wu, & Stuart Sprague

Appendix I: Useful Measurements

Appendix II: Review of Solution Preparation

Appendix III: List of Suppliers

Appendix IV: Common Plant Tissue Culture Terms