Platelets: A Practical Approach

Paperback | October 1, 1982

EditorSteve P. Watson, Kalwant S. Authi

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Platelets are small blood cells that play a vital role in blood clotting. They are essential for wound healing, but their unwanted action can lead to ailments such as stroke and heart attack. In seventeen chapters from leading experts in platelet research this book describes state-of-the-arttechniques for the experienced researcher, both in academia and industry. Methodology is also included that will enable the newcomer to perform routine experiments. Techniques covered range from basic procedures such as platelet preparation to the latest techniques such as monitoring calcium levelsin single cells, protein tyrosine phosphorylation, and PCR.

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Platelets are small blood cells that play a vital role in blood clotting. They are essential for wound healing, but their unwanted action can lead to ailments such as stroke and heart attack. In seventeen chapters from leading experts in platelet research this book describes state-of-the-arttechniques for the experienced researcher, b...

Stephen Watson is at University of Oxford. Kalwant Authi is at Thrombosis Research Institute, London.

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Format:PaperbackDimensions:396 pages, 9.21 × 6.14 × 0.83 inPublished:October 1, 1982Publisher:Oxford University Press

The following ISBNs are associated with this title:

ISBN - 10:0199635374

ISBN - 13:9780199635375

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Table of Contents

PrefaceAcknowledgement1. Platelet preparation and functional responses2. Cellular model systems to study megakaryocyte differentiation3. Techniques in the preparation of semi-permeable platelets and their uses4. Use of fluorescent indicators to measure intracellular Ca2+ and other ions5. Preparation of highly purified human platelet plasma and intracellular membranes using high voltage free flow electrophoresis and methods to study Ca2+ regulation6. The use of flow-cytometry to study platelet activation7. Identification, isolation, and characterization of platelet glycoproteins mediating platelet adhesion8. Monoclonal antibodies to platelet cell surface antigens and their use9. Measurement of protein phosphorylation, kinase activity, and G protein function in intact platelets and membrane preparations10. Tyrosine phosphorylation11. Study of proteins associated with the platelet cytoskeleton12. Methods for measuring agonist-induced phosphlipid metabolism in intact human platelets13. Cyclic nucleotides: measurement and function14. Immunocytochemical and electron microscope studies of platelets15. Clinical aspects of platelet function16. Platelet procoagulent activity and its measurement17. The study of platelet function in other speciesList of suppliersIndex

Editorial Reviews

`recent addition to the well known and much respected Practical Approach Series. ... In common with the other volumes in the series, the book provides detailed and comprehensive laboratory protocols for the study of all aspects of platelet function. ... This is not a book for the merelycurious, however. it is aimed squarely at those with a determined research interest in platelet function or signal transduction. ... The clear and crisp descriptions are sufficient to allow a comparative novice in the field to establish the methods with relative ease. ... Platelets should proveuseful to anyone with an interest in the biochemistry of cellular signal transduction.'British Journal of Biomedical Science 1997