Platelets and Megakaryocytes: Volume 1: Functional Assays by Jonathan M. GibbinsPlatelets and Megakaryocytes: Volume 1: Functional Assays by Jonathan M. Gibbins

Platelets and Megakaryocytes: Volume 1: Functional Assays

EditorJonathan M. Gibbins, Martyn P. Mahaut-Smith

Paperback | November 10, 2010

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For both volumes:A comprehensive collection of cutting-edge methods for the molecular and cellular analysis of platelets and megakaryocytes. Volume 1 details basic and advanced techniques for analyzing platelet and megakaryocyte function. The approaches presented for platelet analysis include aggregometry, secretion, arachidonic acid metabolism, procoagulent response, platelet adhesion under static and flow conditions, flow cytometry, and production of microparticles. The techniques for study of megakaryocyte function allow the measurement of specialized structures for platelet production, the appearance of platelet-specific surface receptors, and the increase in ploidy. Volume 2 offers techniques for studying many aspects of signaling in platelets and megakaryocytes, as well as state-of-the-art commentaries on our understanding of these cells. The methods include ligand-binding assays, the study of protein and lipid kinases and phosphatases, the analysis of lipid rafts, and the measurement of intracellular calcium levels. There are also techniques for study of electrophysiological events, nitric oxide signaling, venom proteins, and for the internalization of proteins into platelets through permeabilization. This volume also describes the application of molecular and post-genomic methods for the study of platelets and megakaryocytes. All protocols follow the successful Methods in Molecular BiologyT series format, each one offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of equipment and reagents, and tips on troubleshooting and avoiding known pitfalls. The two volumes offer novice and experienced cell biologists, hematologists, and clinicians not only a benchmark survey of the field, but also a comprehensive library of proven techniques essential for productive research on platelets and megakaryocytes today.
Title:Platelets and Megakaryocytes: Volume 1: Functional AssaysFormat:PaperbackDimensions:406 pagesPublished:November 10, 2010Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617373567

ISBN - 13:9781617373565

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Table of Contents

Part I. Platelet Functional AssaysEffects of Anticoagulants Used During Blood Collection on Human Platelet FunctionJane A. May and Stanley HeptinstallPreparation of Washed Platelet Suspensions From Human and Rodent BloodJean-Pierre Cazenave, Philippe Ohlmann, Dominique Cassel, Anita Eckly, Béatrice Hechler, and Christian GachetPlatelet CountingPaul Harrison, Carol Briggs, and Samuel J. MachinElectron Microscopy Methods for Studying Platelet Structure and FunctionJames G. WhitePlatelet Aggregation: Turbidimetric MeasurementsGavin E. JarvisPlatelet Aggregation in Whole Blood: Impedance and Particle Counting MethodsGavin E. JarvisSecretion From Dense Granules: Luminescence Method for Adenine NucleotidesM. Fred HeathPlatelet Dense-Granule Secretion: The [3H]-5-HT Secretion AssayDavid Crosby and Alastair W. PooleAnalysis of Releasable Nucleotides of PlateletsJoachim Jankowski, Lars Henning, and Hartmut SchlüterStudies of Secretion Using Permeabilized PlateletsTara W. Rutledge and Sidney W. WhiteheartMeasurement of Platelet Arachidonic Acid MetabolismRichard W. Farndale, Philip G. Hargreaves, Joanna L. Dietrich, and Rosemary J. KeoghMeasurement of the Platelet Procoagulant ResponseJohan W. M. Heemskerk, Paul Comfurius, Marion A. H. Feijge, and Edouard M. BeversPlatelet Adhesion Assays Performed Under Static ConditionsJoanne M. StevensPlatelet Adhesion Assays Under Flow Using Matrix Protein-Coupled Adhesion ColumnsRenata Polanowska-Grabowska and Adrian R. L. GearTechniques to Examine Platelet Adhesive Interactions Under FlowSuhasini Kulkarni, Warwick S. Nesbitt, Sacha M. Dopheide, Sascha C. Hughan, Ian S. Harper, and Shaun P. JacksonIn Vivo Models of Platelet Function and Thrombosis: Study of Real-Time Thrombus FormationShahrokh Falati, Peter L. Gross, Glenn Merrill-Skoloff, Derek Sim, Robert Flaumenhaft, Alessandro Celi, Barbara C. Furie, and Bruce FurieAdhesion Between Platelets and Leukocytes or Endothelial CellsGerard B. NashIn Vitro Measurement of High-Shear Platelet Adhesion and Aggregation by the PFA-100®Paul HarrisonFlow-Cytometric Analysis of Platelet-Membrane Glycoprotein Expression and Platelet ActivationAlison H. Goodall and Jackie ApplebyFlow-Cytometric Analysis of Mouse Platelet FunctionBernhard Nieswandt, Valerie Schulte, and Wolfgang BergmeierPlatelet MicroparticlesShosaku Nomura and Shirou FukuharaPart II. Megakaryocyte Functional AssaysIsolation of Primary Megakaryocytes and Studies of Proplatelet FormationRobert M. LevenIsolation and Culture of Megakaryocyte PrecursorsNajet Debili, Fawzia Louache, and William VainchenkerAssays of Megakaryocyte Development: Surface Antigen Expression, Ploidy, and SizeAnthony Mathur, Ying Hong, Guosu Wang, and Jorge D. ErusalimskyAssays of Megakaryocyte Development: Cytoplasm, Storage Granules, and Demarcation MembranesArnaud Drouin, Gulie Alimardani, and Elisabeth M. CramerThrombopoietin BioassayWarren S. Alexander and Craig HylandCulture of Megakaryocytic Cell Lines: Uses and LimitationsNorio KomatsuIndex

Editorial Reviews

"...thorough and detailed...contains useful information regarding the study of platelets and megakaryocytes." - Veterinary Pathology