Potassium Channels: Methods and Protocols by Jonathan D. LippiatPotassium Channels: Methods and Protocols by Jonathan D. Lippiat

Potassium Channels: Methods and Protocols

EditorJonathan D. Lippiat

Paperback | November 19, 2010

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Due to the importance of potassium channels in cellular function, researchers have been challenged to develop tools and experimental procedures to probe the function and identify chemicals that modulate the behaviour of this super-family of ion channels. In Potassium Channels: Methods and Protocols, experts in the field present a range of experimental approaches that have been developed to investigate potassium channel structure, function, pharmacology, cell biology, gene expression, and their role in disease. Many of these techniques study potassium channels as cellular proteins as well as the resultant membrane biophysics. Written in the highly successful Methods in Molecular BiologyT series format, the chapters contain brief introductions to the subject, lists of the necessary materials and reagents, readily reproducible laboratory protocols, and a Notes section, which highlights tips on troubleshooting and avoiding known pitfalls.Thorough and cutting-edge, Potassium Channels: Methods and Protocols provides a wide range of approaches in order to aid current research projects and inspire new investigations.
Title:Potassium Channels: Methods and ProtocolsFormat:PaperbackDimensions:312 pages, 10.24 × 7.6 × 0 inPublished:November 19, 2010Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617379409

ISBN - 13:9781617379406

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Table of Contents

Part I: Methods for Studying Potassium Channel Transcription and mRNA SplicingIdentifying Transcriptional Regulatory Regions Using Reporter Genes and DNA-Protein Interactions by Chromatin Immunoprecipitation Lezanne Ooi and Ian C. WoodQuantitative RT-PCR Methods for Investigation of Low Copy Potassium Channel Gene Expression in Native Murine Arteries Alex Cheong, Samuel J. Fountain, and David J. BeechCloning of Potassium Channel Splice Variants from Tissues and Cells Lie Chen and Michael J. Shipston Part II: Methods for Studying the Cell Biology of Potassium Channel ProteinsChemiluminescence Assays to Investigate Membrane Expression and Clathrin Mediated Endocytosis of KATP Channels Andrew J. Smith and Asipu SivaprasadaraoInvestigation of KATP Channel Endocytosis by Immunofluorescence Andrew J. Smith and Asipu SivaprasadaraoInvestigation of KATP Channel Endocytosis and Cell Surface Density by Biotinylation and Western Blotting Andrew J. Smith and Asipu SivaprasadaraoLipid Microdomains and K+ Channel Compartmentation: Detergent and Non-Detergent-Based Methods for the Isolation and Characterisation of Cholesterol-Enriched Lipid Rafts Laura J. Sampson and Caroline DartDetermination of Phosphoinositide Binding to K+ Channel Subunits Using a Protein Lipid Overlay Assay Alison M. Thomas and Andrew TinkerProtein Complex Analysis of Native Brain Potassium Channels by Proteomics Guillaume Sandoz and Florian Lesage Part III: Electrophysiological Techniques for the Study of Potassium Channel FunctionXenopus Oocytes as a Heterologous Expression System for Studying Ion Channels with the Patch Clamp Technique Paolo Tammaro, Kenju Shimomura, and Peter ProksWhole Cell Recording Using the Perforated Patch Clamp Technique Jonathan D. LippiatRecording the Activity of ATP-Sensitive K+ Channels in Open-Cell Cell-Attached Configuration Andrei I. TarasovPlanar Patch Clamp: Advances in Electrophysiology Andrea Brüggemann, Cecilia Farre, Claudia Haarmann, Ali Haythornthwaite, Mohamed Kreir, Sonja Stoelzle, Michael George, and Niels FertigAnalyzing Steroid Modulation of BKCa Channels Reconstituted into Planar Lipid Bilayers Heidi de Wet, Jonathan D. Lippiat, and Marcus Allen Part IV: Optical Techniques for the Study of Potassium Channel Function and InteractionsUsing Bioluminescence Resonance Energy Transfer (BRET) to Measure Ion Channel Assembly Gina M. Whitaker and Eric A. AcciliThe Use of FRET Microscopy to Elucidate Steady State Channel Conformational Rearrangements and G Protein Interaction with the GIRK Channels Adi Raveh, Inbal Riven, and Eitan ReuvenyThe Voltage-Clamp Fluorometry Technique Chris S. Ganhdi and Riccardo Olcese  Part V: Methods for Studying Potassium Channelopathies and Pharmacological ModulatorsIdentification of Mutations in the Kir6.2 Subunit of the KATP Channel Sarah E. Flanagan and Sian EllardModulation of Potassium Ion Channel Proteins Utilizing Antibodies Mark L Dallas, Susan A. Deuchars, and Jim DeucharsFluorescence-Based Tl+-Influx Assays as a Novel Approach for Characterization of Small-Conductance Ca2+-Activated K+ Channel Modulators Susanne Jørgensen, Tina H. Johansen, and Tino DyhringRubidium Efflux as a Tool for the Pharmacological Characterisation of Compounds with BK Channel Opening Properties Neil G. McKay, Robert W. Kirby, and Kim LawsonRecording hERG Potassium Currents and Assessing the Effects of Compounds Using the Whole-Cell Patch Clamp Technique           Ray M. Helliwell

Editorial Reviews

From the reviews:"This is a very informative book, covering a variety of methods available to study potassium channels . . The protocols are clearly organized and usefully detailed. . Illustrative figures and in some cases, photographs of equipment . adding to the clarity of the methods sections. This book . a valuable reference not only for those already in the potassium channel field but also for studies of other ion channel systems. . each chapter allows bench scientists to undertake new, informed, experimental approaches in their research." (Samantha Miller, Microbiology Today, July, 2009)"This book is dedicated to experimental methods, which allow us to investigate potassium channels from their genes through gene transcription to protein structure, function and regulation in health and disease. . chapters in this book are written in form of practical laboratory protocols. Therefore the book will be primarily used by practicing scientists and by graduated and post-graduate students for their practical research. Certainly it will find its place in many laboratories investigating various features of potassium channels regulations." (Lubica Lacinová, General Physiology and Biophysics, Vol. 29, April, 2010)