Protein Expression: A Practical Approach

Paperback | March 1, 1999

EditorS. J. Higgins, B. D. Hames

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Protein Expression: A Practical Approach and its companion volume Post-translational Modification: A Practical Approach complete the mini-series of Practical Approach books covering the synthesis and subsequent processing of proteins. Protein Expression: A Practical Approach details theexpression of cloned DNA or RNA templates in all the major in vivo and in vitro systems. The in vivo systems covered are cultured mammalian cells, the yeasts Saccharomyces cerevisiae and Pichia pastoris, baculovirus, Xenopus oocytes, and prokaryotic cells. Cell-free systems of both eukarytotes andprokaryotes are described, including the prokaryotic systems that offer copuled transcription- translation. There is also a chapter on monitoring protein expression. The post- translational fate of proteins is covered in Post-Translational Processing: A Practical Approach.

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Protein Expression: A Practical Approach and its companion volume Post-translational Modification: A Practical Approach complete the mini-series of Practical Approach books covering the synthesis and subsequent processing of proteins. Protein Expression: A Practical Approach details theexpression of cloned DNA or RNA templates in all ...

David Hames is at University of Leeds. Steve Higgins is at University of Leeds.

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Format:PaperbackPublished:March 1, 1999Publisher:Oxford University PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:0199636230

ISBN - 13:9780199636235

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Table of Contents

1. Protein expression in mammalian cells2. Expression in Xenopus oocytes and cell-free extracts3. Expressing cloned genes in the yeasts Saccharomyces cerevisiae and Pichia pastoris4. Baculovirus expression systems5. Protein synthesis in eukaryotic cell-free systems6. Prokaryotic in vivo expression systems7. Cell-free coupled transcription-translation systems from Escherischia Coli8. Monitoring protein expression

Editorial Reviews

' The earlier volume on Transcription and Translation has been well used in my laboratory, and I am sure this update will become equally well thumbed' Microbiology Today