Protein Localization by Fluorescence Microscopy: A Practical Approach

Paperback | November 18, 1999

EditorViki Allan

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There is an ever-increasing number of genes that have been sequenced but are of completely unknown function. The ability to determine the location of such gene products within the cell, either by the use of antibodies or by the production of chimeras with green fluorescent protein, is avital step towards understanding what they do. This is one major reason why fluorescence microscopy is enjoying a revival. This no-nonsense guide provides detailed, practical advice on all aspects of the subject: from choosing the right equipment, to interpreting results. It balances theadvantages of a wide range of techniques - including live cell work - against the potential pitfalls, offering invaluable "tricks of the trade" along the way. Protein Localization by Fluorescence Light Microscopy: A Practical Approach has something to offer all microscopists, giving a solidgrounding to the novice whilst extending the range of the experienced user.

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There is an ever-increasing number of genes that have been sequenced but are of completely unknown function. The ability to determine the location of such gene products within the cell, either by the use of antibodies or by the production of chimeras with green fluorescent protein, is avital step towards understanding what they do. T...

Viki Allan is at University of Manchester.
Format:PaperbackPublished:November 18, 1999Publisher:Oxford University PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:0199637407

ISBN - 13:9780199637409

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Table of Contents

Preface1. Allan: Basic Immunofluorescence2. North and Small: Immunofluorescent Labelling of Sections3. Ersfeld and Stone: 4. Amos: Instruments for Fluorescence Imaging5. Pepperkok and Shima: Fluorescence Microscopy of Living Vertebrate Cells6. Davis: Visualising Fluorescence in Drosophila - optimal detection in thick specimens7. Hawes, Boevink and Moore: Green Fluorescent Protein in Plants8. Hagan and Ayscough: Fluorescence Microscopy in YeastIndex

Editorial Reviews

" ... a useful bencjh-aid. The Editor-Victoria Allan - ans all of the contributors should be congratulated on putting together a valuable reference text " Trends Cell Biology