Protein Phosphorylation: A Practical Approach

Paperback | October 14, 1999

EditorGrahame Hardie

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Reversible phosphorylation is one of the major mechanisms of controlling protein activity in all eukaryotic cells. This new edition of Protein Phosphorylation: A Practical Approach provides a comprehensive description of current methods used to study protein phosphorylation and the kinases andphosphatases which catalyse it. It includes protocols for studying phosphorylation in intact cells; analysis of signal transduction pathways, kinase specificity, and kinase interactions; assay and purification of kinases and phosphatases; and identification of substrates. Also covered are cloningand expression protocols and advice on the crystallization of kinases and phosphatases. Protein Phosphorylation: A Practical Approach 2e will therefore be of great value to any researcher investigating aspects of reversible protein phosphrylation.

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Reversible phosphorylation is one of the major mechanisms of controlling protein activity in all eukaryotic cells. This new edition of Protein Phosphorylation: A Practical Approach provides a comprehensive description of current methods used to study protein phosphorylation and the kinases andphosphatases which catalyse it. It includes...

Grahame Hardie is at University of Dundee.
Format:PaperbackPublished:October 14, 1999Publisher:Oxford University PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:0199637288

ISBN - 13:9780199637287

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Table of Contents

Preface1. Haystead and Garrison: Study of protein phosphorylation in intact cells2. Naito, Kobayashi and Hidaka: Analysis of signal transduction pathways using protein kinase inhibitors and activators3. Hardie: Analysis of signal transduction pathways using proteinserine/threonine phosphatase inhibitors4. Sutherland: Analysis of signal transduction pathways using molecular biological manipulations5. Van der Geer et al: Phosphopeptide mapping and phosphoamino acid analysis on cellulose thin-layer plates6. Mitchellhill and Kemp: Phosphorylation site analysis by mass spectrometry7. Mackintosh and Moorhead: Assay and purification of protein (serine/threonine) phosphatases8. Tonks: [chapter title missing]9. Garton, Flint and Tonks: Identification of substrates for protein-tyrosine phosphatases10. Hardie et al: Assay and purification of protein-serine/threonine kinases11. Ruzzene and Pinna: Assay of protein kinases and phosphatases using specific peptide substrates12. Carling: Cloning and expression of cDNAs encoding protein kinase subunits13. Stone and Walker: Interaction cloning of protein kinase partners14. Fukunaga and Hunter: Identifying protein kinase substrates by expression screening with solid-phase phosphorylation15. Jiang and Carlson: Analysis of protein kinase interactions using the two-hybrid method16. Herberg and Zimmerman: Analysis of protein kinase interactions using biomolecular interaction analysis17. Songyang and Cantley: Analysis of protein kinase specificity using orientated peptide libraries18. Kobe et al: Crystallization of protein kinases and phosphatasesIndex

Editorial Reviews

"This will be an invaluable laboratory aid to researches involved in this field" Aslib Book Guide