Protein Purification: Principles and Practice

Hardcover | November 19, 1993

byRobert K. Scopes

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The third edition of this classic guide to protein purification updates methods, principles and references. As in the widely-acclaimed earlier editions, Scopes guides both the novice and the experienced researcher from theory to application. Using the book, the reader is able to integrate methods effectively into optimum protocols for the task at hand.Reviews of earlier editions: "good practical advice that is presented in a pleasantly readable form" --Analytical Biochemistry"well organized and written clearly" --American Scientist"should be on every laboratory shelf where protein are being handled or purified...a feast and a genuine pleasure to read" --Nature

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From Our Editors

The third edition of this classic guide updates methods, principles, and references, taking both the novice and the experience researcher from theory to application. "Should be on every laboratory shelf where proteins are being handled or purified . . . a feast and a genuine pleasure to read".--Nature. 164 illustrations

From the Publisher

The third edition of this classic guide to protein purification updates methods, principles and references. As in the widely-acclaimed earlier editions, Scopes guides both the novice and the experienced researcher from theory to application. Using the book, the reader is able to integrate methods effectively into optimum protocols for ...

Format:HardcoverDimensions:399 pages, 9.21 × 6.14 × 0.03 inPublished:November 19, 1993Publisher:Springer New York

The following ISBNs are associated with this title:

ISBN - 10:0387940723

ISBN - 13:9780387940724

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Table of Contents

Prefaces; 1. The Protein Purification Laboratory; 2. Making an Extract; 3. Analysis- Measurement of Protein and Enzyme Activity; 4. Separation by Precipitation; 5. Separation by Adsorption I: General Principles; 6. Separation by Adsorption II: Ion Exchangers and Nonspecific Adsorbents; 7. Separation by Adsorption: Affinity Techniques; 8. Separation in Solution; 9. Purification of Special Types of Proteins; 10. Small-Scale and Large-Scale Procedures; 11. Analysis for Purity; 12. Optimization of Procedures: Final Steps; Appendix A-D; References; Index

From Our Editors

The third edition of this classic guide updates methods, principles, and references, taking both the novice and the experience researcher from theory to application. "Should be on every laboratory shelf where proteins are being handled or purified . . . a feast and a genuine pleasure to read".--Nature. 164 illustrations

Editorial Reviews

From reviews of earlier editions: "good practical advice that is presented in a pleasantly readable form" (Analytical Biochemistry), "well organized and written clearly" (American Scientist), "should be on every laboratory shelf where protein are being handled or purified...a feast and a genuine pleasure to read" (Nature)