It has been known for many years that the subcellular distribution of proteins throughout eukaryotic cells is highly ordered. This means that proteins have to be transported to their correct destinations and left there. Understanding the complex mechanisms by which this occurs has provided amajor challenge for modern biology. Recent progress in the subject has relied heavily on finding ways to measure protein transport and targeting. Protein Targeting brings together contributions from active researchers in the field, who provide detailed step-by-step protocols for studying the processes involved, using both intactcells and broken cell preparations. Cell biological, biochemical, and molecular biological methods are all addressed in detail, and the chapters cover the well-established techniques, as well as a number of exciting new methodologies which have potentially very wide applications. This book willenable scientists from varied backgrounds to get to grips with unravelling the mechanisms which control cellular protein targeting.