Proteolytic Enzymes: A Practical Approach by Rob Beynon

Proteolytic Enzymes: A Practical Approach

EditorRob Beynon, J. S. Bond

Paperback | December 31, 2000

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Like the popular first edition, this new edition of Proteolytic Enzymes emphasizes practical aspects of the handling, characterization, inhibition, and use of proteolytic enzymes giving general advice and specific examples. The text and protocols have been thoroughly updated to take account ofthe advances made in the last 10 years in both the increased understanding of the role of peptidases in many critical cellular processes e.g. apoptosis and new technological developments e.g. in recombinant protein expression, protein sequencing, and structural studies. The topics covered are:nomenclature and classification; purification; assay methods; determination of mechanism; inhibition and prevention of unwanted proteolytic activity; characterizing natural inhibitors; proteolytic enzymes in peptide mapping and primary structure elucidation by mass spectrometry and Edman sequencing;limited proteolysis as a structural probe; synthetic function. This book will be as invaluable as the first edition in providing ideas and protocols for scientists either studying proteases or using proteases as a research tool.

About The Author

Rob Beynon is at University of Liverpool. J. S. Bond is at The Pennsylvania State University.

Details & Specs

Title:Proteolytic Enzymes: A Practical ApproachFormat:PaperbackDimensions:358 pages, 9.69 × 7.44 × 0.79 inPublished:December 31, 2000Publisher:Oxford University PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:0199636621

ISBN - 13:9780199636624

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Table of Contents

Proteolytic Enzymes: nomenclature and classificationPurification of Proteolytic EnzymesProtease Assay MethodsDetermination of Protease MechanismInhibition of Proteolytic EnzymesFinding, Purification and Characterization of Natural Protease InhibitorsMass Spectrometry of Proteolysis-derived Peptides for Protein IdentificationUsing Proteinases for Edman Sequence Analysis and Peptide MappingPrevention of Unwanted ProteolysisProteolysis of Native Proteins as a Structural ProbeProteases in Peptide Synthesis

Editorial Reviews

"the second edition of Proteolytic enzymes;a practical approach is a welcome update to the volume of the same name ... good background to newcomers and students in the field, and will serve as a reference book for anyone working in ths area of research," Biochemistry and MolecularBiology