Pulsed Field Gel Electrophoresis: A Practical Approach

Paperback | October 1, 1995

EditorA. P. Monaco

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PFGE is the most reliable method for separating large fragments of DNA and has had a major impact on biological research. This book presents detailed chapters on both the theory behind PFGE amd protocols for most of its major research and diagnostic applications.

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From the Publisher

PFGE is the most reliable method for separating large fragments of DNA and has had a major impact on biological research. This book presents detailed chapters on both the theory behind PFGE amd protocols for most of its major research and diagnostic applications.

From the Jacket

Pulsed Field Gel Electrophoresis: A Practical Approach is a unique compendium of expert advice, detailed methodology, and hints and tips for using PFGE in a wide range of research strategies. It is an invaluable lab-bench manual for all those researchers who use PFGE or who plan to do so.

A. P. Monaco is at John Radcliffe Hospital, Oxford.

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Format:PaperbackDimensions:216 pages, 9.21 × 6.14 × 0.43 inPublished:October 1, 1995Publisher:Oxford University Press

The following ISBNs are associated with this title:

ISBN - 10:0199635358

ISBN - 13:9780199635351

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Table of Contents

1. Theories of gel electrophoresis of high molecular weight DNA2. PFGE in physical mapping3. Mutation detection and diagnosis using PFGE4. Cloning from PFGE-purified genomic DNA fragments and YACs5. Yeast artificial chromosome cloning using PFGE6. PFGE analysis of yeast artificial chromosomes7. Functional analysis of mammalian genomes using yeast artificial chromosomes8. PFGE in the study of a bacterial pathogen (Haemophilus influenzae)9. Analysis of the genomes of protozoan parasites using PFGE

Editorial Reviews

`All nine chapters treat all these problems which will certainly meet a great success.'Celular and Molecular Biology, Vol. 42, No. 3, 1996