Quantitative Real-time Pcr: Methods And Protocols by Roberto BiassoniQuantitative Real-time Pcr: Methods And Protocols by Roberto Biassoni

Quantitative Real-time Pcr: Methods And Protocols

byRoberto BiassoniEditorAlessandro Raso

Hardcover | October 29, 2019

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This book expands upon the useful first edition by exploring classic Quantitative Polymerase Chain Reaction (qPCR) techniques as well as a number of recently developed applications. With the changes in instrumentation due to technological advances and the development of new reagents to fulfill ethical and legal issues, the qPCR field is now an up-to-date technology that indeed is widely used in research and clinical diagnostics. Written for the highly successfulMethods in Molecular Biologyseries, chapters include introductions to their respective topics, lists of the necessary reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. 

Revised and authoritative,Quantitative Real-Time PCR: Methods and Protocols, Second Editionis an ideal guide to this expanding and vital field of study.
Title:Quantitative Real-time Pcr: Methods And ProtocolsFormat:HardcoverDimensions:205 pagesPublished:October 29, 2019Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1493998323

ISBN - 13:9781493998326


Table of Contents

1. A Quarter Century and the PCR Applied Techniques and the Fields of Use Are Still Increasing in Numbers

            Alessandro Raso and Roberto Biassoni


2. Parameters for Successful PCR Primer Design

            Stephen A. Bustin, Reinhold Mueller, and Tania Nolan


3. MIQE-Compliant Validation of MicroRNA Biomarker Signatures Established by Small RNA Sequencing

            Veronika Mussack, Stefanie Hermann, Dominik Buschmann, Benedikt Kirchner, and Michael W. Pfaffl


4. Enhanced Probe-Based RT-qPCR Quantification of MicroRNAs Using Poly(A)tailing and 5' Adaptor Ligation

            Valentin Vautrot and Isabelle Behm-Ansmant


5. A Novel System to Discriminate HLA-C mir148a Binding Site by Allele-Specific Quantitative PCR

            Priscilla Biswas, Eddi Di Marco, and Mauro S. Malnati


6. Detection of Yellow Fever Virus by Quantitative Real-Time PCR (qPCR)

            Gisela Freitas Trindade, Sheila Maria Barbosa de Lima, Constança Britto, and Alice Gomes Fernandes Monteiro


7. Evaluation of the Abundance of Fungi in Wastewater Treatment Plants Using Quantitative PCR (qPCR)

            Paula Maza-Márquez, Elisabet Aranda, Jesús González-López, and Belén Rodelas


8. Early Detection of Fungal Plant Pathogens by Real-Time Quantitative PCR: The Case ofDiplodia sapineaon Pine

            Nicola Luchi, Alberto Santini, Francesca Salvianti, and Pamela Pinzani


9. A General Protocol for Accurate Gene Expression Analysis in Plants

            Ellen De Keyser, Laurence Desmet, Magali Losschaert, and Jan De Riek


10. Gene Expression Analysis in Bacteria by RT-qPCR

            Danilo J.P.G. Rocha, Thiago L.P. Castro, Eric R.G.R. Aguiar, and Luis G.C. Pacheco


11. Detection and Characterization of Circulating Tumor Cells by Quantitative Real-Time PCR

            Francesca Salvianti, Filomena Costanza,Gemma Sonnati, and Pamela Pinzani


12. Molecular Monitoring of Chronic Myeloid Leukemia

            Katherine Dominy, Katya Mokretar, Alistair G. Reid, and Jamshid S. Khorashad


13. Normalization in Human Gliomas Tissue

            Ana Paula Santin Bertoni, Isabele Cristina Iser Marson, Rafael Paschoal de Campos, and Márcia Rosangela Wink


14. qPCR Applications for the Determination of the Biological Age

            Mauro Castagnetta, Ulrich Pfeffer, Aldo Chiesa, Elena Gennaro, Massimiliano Cecconi, Domenico Coviello, and Nicoletta Sacchi


15. QuantStudio'¢ 12K Flex OpenArray® System as a Tool for High-Throughput Genotyping and Gene Expression Analysis

            Chiara Broccanello, Letizia Gerace, and Piergiorgio Stevanato


16. Digital PCR and the QuantStudio'¢ 3D Digital PCR System

            Marion Laig, Christie Fekete, and Nivedita Majumdar