Recombinant and In Vitro RNA synthesis: Methods and Protocols by Graeme L. ConnRecombinant and In Vitro RNA synthesis: Methods and Protocols by Graeme L. Conn

Recombinant and In Vitro RNA synthesis: Methods and Protocols

EditorGraeme L. Conn

Hardcover | October 12, 2012

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The discovery of catalytic RNAs in the mid-1980s marked the beginning of a new era in RNA biology and an ever increasing appreciation of the diverse and critical roles played by this fascinating molecule. In Recombinant and In Vitro RNA Synthesis: Methods and Protocols, expert researchers in the field detail approaches from the inception of a new RNA project to the final sample ready for analysis. These experimental protocols combine established work-horse approaches with some of the latest innovative methods for RNA synthesis and purification. Written in the highly successful Methods in Molecular BiologyT series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Recombinant and In Vitro RNA Synthesis: Methods and Protocols aids scientists in continuing to study the fascinating world of RNA.
Title:Recombinant and In Vitro RNA synthesis: Methods and ProtocolsFormat:HardcoverDimensions:288 pagesPublished:October 12, 2012Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:162703112X

ISBN - 13:9781627031127

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Table of Contents

1. Purification of RNA Expressed in vivo Inserted in a tRNA Scaffold  Luc Ponchon and Frédéric Dardel    2. Selective RNase H Cleavage of Target RNAs  from a tRNA Scaffold Luc Ponchon, Geneviève Beauvais, Sylvie Nonin-Lecomte and Frédéric Dardel    3. Preparation of Long Templates for RNA in vitro Transcription by Recursive PCR Jessica C. Bowman, Bahareh Azizi, Timothy K. Lenz, Poorna Roy, and Loren D. Williams   4. General Protocols for Preparation of Plasmid DNA Template, RNA in vitro Transcription and RNA Purification by Denaturing PAGE Jo L. Linpinsel and Graeme L. Conn   5. Preparation of Short RNA by in vitro Transcription Cheng Lu and Pingwei Li   6. Native RNA Purification by Gel Filtration Chromatography Evan P. Booy, Hui Meng and Sean A. McKenna  7. Cis-acting Ribozymes for the Production of RNA in vitro Transcripts with Defined 5' and 3' Ends Johanna M. Avis, Graeme L. Conn, and Scott C. Walker   8. Trans-acting Antigenomic HDV Ribozyme for Production of in vitro Transcripts with Homogenous 3' Ends  Milena Szafraniec, Leszek Blaszczyk, Jan Wrzesinski, and Jerzy Ciesiolka   9.  Rapid Preparation of RNA Samples using DNA-affinity Chromatography and DNAzyme Methods  Hae-Kap Cheong, Eunha Hwang, and Chaejoon Cheong   10.  Preparation of lN-GST Fusion Protein for Affinity Immobilization of RNA Geneviève Di Tomasso, Philipe Lampron, James G. Omichinski and Pascale Legault   11. Affinity Purification of RNA using an ARiBo Tag Geneviève Di Tomasso, Pierre Dagenais, Alexandre Desjardins, Alexis Rompré-Brodeur, Vanessa Delfosse and Pascale Legault   12. Plasmid Template Design and in vitro Transcription of Short RNAs within a 'Structure Cassette' for Structure Probing Experiments Virginia K. Vachon and Graeme L. Conn   13. In Vitro Transcription of Modified RNAs Stephanie L. Moon and Jeffrey Wilusz   14. End-Labeling Oligonucleotides with Chemical Tags After Synthesis N. Ruth Zearfoss and Sean P. Ryder   15. High-Purity Enzymatic Synthesis of  Site-Specifically Modified tRNA Ya-Ming Hou   16. Se-derivatized RNAs for X-ray Crystallography Lina Lin and Zhen Huang   17. Biosynthetic Preparation of 13C/15N-labelled rNTPs for High-resolution NMR Studies of RNAs Luigi Martino and Maria R Conte   18. Preparative Separation of Ribonucleoside Monophosphates by Ion-Pair Reverse-Phase HPLC Pierre Dagenais and Pascale Legault  19. Splint Ligation of RNA with T4 DNA Ligase Christopher J. Kershaw and Raymond T. O'Keefe