Recombinant Protein Production in Yeast: Methods and Protocols by Roslyn M. BillRecombinant Protein Production in Yeast: Methods and Protocols by Roslyn M. Bill

Recombinant Protein Production in Yeast: Methods and Protocols

EditorRoslyn M. Bill

Hardcover | March 28, 2012

Pricing and Purchase Info

$180.95

Earn 905 plum® points

In stock online

Ships free on orders over $25

Not available in stores

about

In the last few years, significant advances have been made in understanding how a yeast cell responds to the stress of producing a recombinant protein, and how this information can be used to engineer improved host strains. The molecular biology of the expression vector, through the choice of promoter, tag and codon optimization of the target gene, is also a key determinant of a high-yielding protein production experiment. Recombinant Protein Production in Yeast: Methods and Protocols examines the process of preparation of expression vectors, transformation to generate high-yielding clones, optimization of experimental conditions to maximize yields, scale-up to bioreactor formats and disruption of yeast cells to enable the isolation of the recombinant protein prior to purification. Written in the highly successful Methods in Molecular BiologyT series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Recombinant Protein Production in Yeast: Methods and Protocols, seeks to aid scientists in adopting yeast as a protein production host.
Title:Recombinant Protein Production in Yeast: Methods and ProtocolsFormat:HardcoverDimensions:260 pages, 10 × 7.01 × 0 inPublished:March 28, 2012Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617797693

ISBN - 13:9781617797699

Look for similar items by category:

Customer Reviews of Recombinant Protein Production in Yeast: Methods and Protocols

Reviews

Table of Contents

1 Optimising Yeast as a Host for Recombinant Protein Production( review) Nicklas Bonander and Roslyn M Bill2 Which Yeast Species Shall I Choose? Saccharomyces cerevisiae versus Pichia pastoris (review) Richard AJ Darby, Stephanie P Cartwright, Marvin V. Dilworth and Roslyn M Bill3 Preparation of Pichia pastoris Expression PlasmidsChristel Logez, Fatima Alkhalfioui, Bernadette Byrne, Renaud Wagner4 Preparation of Saccharomyces cerevisiae Expression PlasmidsDavid Drew and Hyun Kim5 Codon Optimisation for Heterologous Gene Expression in YeastKristina Hedfalk6 Yeast Transformation to Generate High-yielding ClonesMohammed Jamshad and Richard AJ Darby7 Screening for High-yielding Pichia pastoris Clones: the Production of G Protein-Coupled Receptors as a Case StudyShweta Singh, Adrien Gras, Cedric Fiez-Vandal, Magdalena Martinez, Renaud Wagner, Bernadette Byrne8 Screening for High-yielding Saccharomyces cerevisiae Clones: Using a Green Fluorescent Protein Fusion Strategy in the Production of Membrane ProteinsDavid Drew and Hyun Kim9 The Effect of Antifoam Addition on Protein Production YieldsSarah J Routledge and Roslyn M Bill10 Setting up a Bioreactor for Recombinant Protein Production in YeastSarah J Routledge and Michelle Clare11 The Implementation of a Design of Experiments Strategy to Increase Recombinant Protein Yields in Yeast (review)Nagamani Bora, Zharain Bawa, Roslyn M. Bill and Martin D. B. Wilks12 On-line Analysis and Process Control in Recombinant Protein Production (review)Shane M. Palmer and Edmund R.S. Kunji13 Monitoring the Biomass Accumulation of Recombinant Yeast Cultures: Off-line Estimations of Dry Cell Mass and Cell CountsShane Palmer and Edmund R S Kunji14 On-line Monitoring of Biomass Accumulation in Recombinant Yeast CulturesShane Palmer and Edmund R S Kunji15 Optimising P. pastoris InductionZharain Bawa and Richard AJ Darby16 Optimizing S. cerevisiae Induction Regimes David Drew and Hyun Kim17 Large Scale Production of Membrane Proteins in Pichia pastoris: The Production of G Protein-coupled Receptors as a Case StudyShweta Singh, Adrien Gras, Cedric Fiez-Vandal, Magdalena Martinez, Renaud Wagner and Bernadette Byrne18 Large-scale Production of Membrane Proteins in Saccharomyces cerevisiae: Using a Green Fluorescent Protein Fusion Strategy in the Production of Membrane ProteinsDavid Drew and Hyun Kim19 Large Scale Production of Secreted Proteins in Pichia pastorisNagamani Bora20 Disruption of Yeast Cells to Isolate Recombinant ProteinsMohammed Jamshad and Richard AJ Darby