Renal Cancer: Methods and Protocols by Jack H. MydloRenal Cancer: Methods and Protocols by Jack H. Mydlo

Renal Cancer: Methods and Protocols

EditorJack H. Mydlo

Paperback | November 9, 2010

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Jack H. Mydlo, MD, and a panel of leading clinicians and researchers review every aspect of the latest surgical, medical, and immunological techniques that can be used in the diagnosis and treatment of renal cancer. These broadly experienced investigators also present a practical account of their best basic research methods, including the use of reverse transcriptase PCR combined with genomic hybridization, cadherin, and metalloproteinase expression to reveal important factors in the detection, staging, aggressiveness, and treatment of this disease. Gene therapy, the generation of monoclonal antibodies, and the use of interferon alpha, GM-CSF, and IL-6 are also discussed. Comprehensive and pioneering, Renal Cancer: Methods and Protocols offers urologists, medical oncologists, laboratory investigators, and pathologists a practical collection of the major cutting-edge techniques and therapies for renal cancer today, together with a view of the highly promising future of gene therapy.
Title:Renal Cancer: Methods and ProtocolsFormat:PaperbackDimensions:416 pages, 9.02 × 5.98 × 0.07 inPublished:November 9, 2010Publisher:Humana PressLanguage:English

The following ISBNs are associated with this title:

ISBN - 10:1617372080

ISBN - 13:9781617372087

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Table of Contents

Part I. Introduction. Renal Cell Carcinoma: Clinical Features and Management, Paul Russo. Pathology of Kidney Tumors, David J. Grignon. Part II. In Vitro Experimental Techniques. Telomerase Assay in Renal Cancer, William W. Zhang and Laurence H. Klotz. Comparative Genomic Hybridization, Joseph C. Presti, Jr. Analyzing the FHIT Gene by RT-PCR, Western Blotting, and Immunohistochemistry, Raffaele Baffa, Coleen M. Calviello, Teresa Druck, and Leonard G. Gomella. Detection of Apoptosis in Renal Cell Carcinoma, Kenneth J. Pienta and Jeffrey F. Williams. Fluorescence In Situ Hybridization (FISH) to Metaphase and Interphase Chromosomes, Jill A. Macoska. Polymerase Chain Reaction Detection of DNA Sequence Deletions, Jill A. Macoska. Matrix Metalloproteinases and Their Inhibitors, Alexander Kugler, Paul Thelen, and Rolf-Hermann Ringert. Purification of Growth Factor mRNA in Renal Tissues: bFGF-2, FGF-2, TGFa, and EGFR, Jack H. Mydlo. Laser-Capture Microdissection, Greg L. Griewe, Robert Dean, Wei Zhang, Isabella A. Sesterhenn, Shiv Srivastava, and Judd W. Moul. Expression of c-met and WT-1, Leslie Kushner, Pui Yan Chiu, Peter Pinto, and Gary Hal Weiss. Molecular Analysis of the von Hippel-Lindau Disease Gene, Allen Chernoff, Viera Kasparcova, W. Marston Linehan, and Catherine A. Stolle. Experimental Models and Methods in Antibody Targeting of Renal Cell Carcinoma, Robert L. Vessella, D. Scott Wilbur, Kent Buhler, and Paul H. Lange. Part III. In Vivo Experimental Techniques. Orthotopic Model of Renal Cell Carcinoma, David M. Nanus and Dov Engelstein. Murine Animal Model, Nobuyasu Nishisaka, Philo Morse, Richard F. Jones, Ching Y. Wang, and Gabriel P. Haas. Angiogenisis Assays, Jack H. Mydlo. Antiangiogenic Approaches to Renal Cell Carcinoma: Assays for Inhibitors and Clinical Trials, Steven C. Campbell, Frank K. Reiher, and Norman D. Smith, Jr. Part IV. Clinical Trials and Experiments. Interferon Alpha, GM-CSF-Activated T Cells, and IL-6 in Renal Cell Carcinoma, Belur Patel and Arie Belldegrun. Immunotherapeutic Approach to Cancer with Cutaneous DNA Vaccination, Wilbur B. Browne, William G. Hawkins, and Jonathan J. Lewis. Monoclonal Antibodies: Generation and Clinical Use, Egbert Oosterwijk. Treatment Strategies in Metastatic Renal Cell Carcinoma: Cytokines, Vaccines, and Gene Therapy, Peter F. Mulders. Assessment of T-Cell Immune Dysfunction in Patients with Renal Cell Carcinoma, Robert G. Uzzo, Vladimir Kolenko, Andrew C. Novick, and James H. Finke. Transforming Growth Factor-b1 as a Novel Marker of Response to Therapy for Renal Cell Carcinoma, Howard L. Adler. Static and Flow Cytometry, Ralph Madeb, Dov Pode, and Ofer Nativ. Index.

Editorial Reviews

"Virtually all potentially relevant in vitro and in vivo methodologies are addressed in this book. The sections describing the respective techniques are well written and stylistically equivalent...This book will be a useful addition to most institutional libraries. It will also be of some value to experienced investigators who already possess hands-on familiarity with a particular technique, but merely wish to adapt such methodology to renal cell cancer systems." -Doody's Health Sciences Book Review Journal"...the intros are great! The writing style is surprisingly uniform and very readable. There is plentiful reference to clinical reality and a strong emphasis on novel treatments." -ACP News"...the book is well written and the experimental protocols that were included are described in great detail." - Clinical Immunology